Repetitive DNA sequences as probes for phylogenetic analysis in <i>Vicia</i> genus

Frediani, M.; Maggini, F.; Gelati, M. T.; Cremonini, R.

doi:10.1080/00087114.2004.10589420

Cited by 13 publications

(9 citation statements)

References 28 publications

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“…The high economic importance of the genus Vicia has led to a large body of studies on the molecular characterization and investigation of phylogenetic relationships among species that belong to this genus. Various studies have been reported for investigation of phylogenetic relationships among species based on rDNA (Raina and Ogihara 1995), in situ hybridization with repetitive sequences (Navratilova et al 2003), RAPD analysis (Haider et al 2000;Sakowicz and Cieslikowski 2006), repetitive DNA sequences as probes (Frediani et al 2004), capillary electrophoresis (Piergiovanni and Taranto 2005), and SDS-PAGE on seed storage proteins (Mirali et al 2007). However, none of these could resolve the taxonomic problems.…”

Section: Discussionmentioning

confidence: 99%

“…Equally important is the role of legumes to sustainable agriculture, because of their ability to fix soil nitrogen via symbiotic nitrogen-fixation. They also play an important role as a rotation crop with cereal grain and vegetable crops (Arianoutsou and Thanos 1996;Velazquez et al 2010) The genus Vicia L. is a member of the legume tribe Vicieae of the subfamily Papilionoideae (Frediani et al 2004). The temperate and herbaceous genus Vicia L. comprises 210 annual or perennial species that are widely distributed in temperate regions of Europe, Asia, and the Americas (Maxted 1993;Jaaska 2005).…”

Section: Introductionmentioning

confidence: 99%

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Potential use of ITS2 and matK as a Two-Locus DNA Barcode for Identification of Vicia Species

Raveendar

Lee

Park

et al. 2015

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2015 (March) ABSTRACT We investigated the species discriminatory efficiency of the proposed plant barcoding loci ITS2 and matK in Vicia species. In 2011, China Plant BOL Group proposed the addition of nuclear ITS2 to matK be accepted as a 2-locus DNA barcode to classify plant species. The matK region was chosen as a DNA barcode because of its effective species discriminating power, high quality sequence recovery, and easy experimental analysis. Integration of matK sequences into Vicia phylogeny could improve phylogenetic reconstruction of this species. To assess the ability of barcoding loci to resolve Vicia species, we sampled 36 of the taxonomically best known groups in the genus. Topologies of the phylogenetic trees based on ITS2 and matK analyses were similar but a few accessions were placed into distant phylogenetic groups. Neither ITS2 nor matK analyses alone could discriminate some closely related Vicia species. Thus, we have proposed a concatenated data approach to increase the resolving power of ITS2 and used matK as an additional tool for phylogenetic analysis in Vicia because characterization of the nucleotide sequences of the matK region was easier to recover and more cost-effective than those of the ITS region.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential use of ITS2 and matK as a Two-Locus DNA Barcode for Identification of Vicia Species

Raveendar

Lee

Park

et al. 2015

Plant Breed. Biotech.

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“…The genus Vicia L. is a member of the legume tribe Fabeae (sometimes referred to as “Vicieae”) of the subfamily Papilionoideae [1]. Vicia L. comprises approximately 210 species that are widely distributed in temperate regions of Europe, Asia, and the Americas [2].…”

Section: Introductionmentioning

confidence: 99%

Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

et al. 2015

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Abstract:The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.

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“…Relationships among species of the different sections of Vicia have been studied by phenetic analysis of morphological characters (Maxted et al 1991;Maxted 1993Maxted , 1994Maxted , 1995Ohashi 1995, 1997;Maxted and Douglas 1997;Jaaska and Leht 2007;Leht 2009), by isozymes (Jaaska 1997;Leht and Jaaska 2002;Jaaska 2005, Jaaska andLeht 2007), by electrophoretic seed globulin and albumin patterns (Zimniak- Przybylska and Przybylska 1995;Przybylska and Zimniak-Przybylska 1997) and by DNA sequences analyses (Fennell et al 1998;Potokina et al 1999; Van de Wouw et al 2001Frediani et al 2004;Choi et al, 2006;Endo et al 2008). …”

Section: Introductionmentioning

confidence: 99%

Cytological and molecular characterization of Vicia barbazitae Ten. & Guss.

et al. 2011

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Vicia barbazitae, a taxon belonging to section Vicia of subgenus Vicia, was recovered and analysed by cytological, karyological and molecular methods with the aim of both proposing a general characterisation of this species and studying the relationships among the species of section Vicia . Phylogenetic relationships among the species of the section Vicia and those of the sections Microcarinae, Wiggersia and Atossa were also analysed. Automated karyotype analysis has been determined after Feulgen's reaction; chromosome banding was performed by sequence-specific fluorochrome staining. Fluorescent chromosome banding showed CMA(+)/DAPI(-) NOR-associated heterochromatin in the satellite pair. Karyomorphological parameters, based on symmetry indices, the dendrogram of linkage distance constructed on 37 chromosome parameters, as well as the molecular data based on internal transcribed spacer sequences provided information about phylogenetic position of this species inside the section Vicia and among the species belonging to the sections Microcarinae, Wiggersia, Atossa and Vicia. From our karyological and molecular results, it emerges that V. barbazitae can be considered a natural member of section Vicia.

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Repetitive DNA sequences as probes for phylogenetic analysis in Vicia genus

Cited by 13 publications

References 28 publications

Potential use of ITS2 and matK as a Two-Locus DNA Barcode for Identification of Vicia Species

Potential use of ITS2 and matK as a Two-Locus DNA Barcode for Identification of Vicia Species

Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

Cytological and molecular characterization of Vicia barbazitae Ten. & Guss.

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