Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification

Mr, Thomas; Matsumoto, Shogo; Cain, Patricia; Ns, Scott

doi:10.1007/bf00222076

Cited by 213 publications

(115 citation statements)

References 28 publications

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“…The studied varieties, the viticultural areas in which their cultivation is recommended or allowed according to the Greek legislation, and the areas from where the samples were collected are presented in detail in Table 1 The AFLP molecular analysis was conducted as reported by Vos et al (1995), following the AFLP Plant Mapping Protocol by Applied Biosystems (2007), with several modifications . Grapevine DNA was extracted from young and fully expanded leaves according to Thomas et al (1993), with minor modifications. A total of seven primer combinations with three selective nucleotides and fluorescent dye (in the form of EcoRI[Primer-Axx-Dye] and MseI[PrimerCxx]) were used to amplify genomic DNA through the Polymerase Chain Reaction in order to identify and discriminate the selected cultivars (Table 2).…”

Section: Plant Materialsmentioning

confidence: 99%

Ampelographic and Genetic Characterization of Grapevine Varieties (Vitis vinifera L.) of the ‘Mavroudia’ Group Cultivated in Greece

Stavrakaki

Biniari

2017

Not Bot Horti Agrobo

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Twenty-one grapevine varieties grown all over Greece and belonging to 'Mavroudia' group were ampelographically described and genotyped by AFLP molecular analysis in order to discriminate the varieties, synonyms, homonyms and variations of the group. In most cases, the molecular findings confirmed the results of the ampelographic description. In general, and despite the high degree of genetic similarity between certain pairs of the studied cultivars, the group of 'Mavroudia' was characterized as being heterogeneous. From the studied cultivars, 'Kountoura mavri', 'Mavro Spetson' and 'Pappoudes' showed very high degree of genetic similarity, sustaining the hypothesis that the last two are clones of the first. Grapevine cultivar 'Pappoudes' was for the first time ampelographically described and identified as being closely related to 'Kountoura mavri'. High degree of genetic similarity was observed between cultivars 'Gaidouricha' and 'Agiomavritiko', suggesting that they probably originated from the same parent variety through the accumulation of mutations. This may also be true for cultivars 'Mavrokorakas' and 'Kartsiotis'. Also, the results from the statistical analysis showed that 'Mavro Arachovis', 'Mavroudi Voulgarias' and 'Voulgaroudes', despite the relatively high genetic similarity between them, are different. The same applies for the rest of the cultivars studied, while 'Mavro Kalavriton', the most widespread variety of the 'Mavroudia' group, showed the lowest degree of genetic similarity within the all the cultivars studied. The ampelographic description in combination with the molecular method AFLP are effective for the study of the between and within genetic diversity of grapevine cultivars as well as for their identification and discrimination.

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Section: Plant Materialsmentioning

confidence: 99%

Ampelographic and Genetic Characterization of Grapevine Varieties (Vitis vinifera L.) of the ‘Mavroudia’ Group Cultivated in Greece

Stavrakaki

Biniari

2017

Not Bot Horti Agrobo

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“…DNA was extracted from grapevine plantlets grown in vitro using the method of Thomas et al (1993). PCR amplification was performed using specific primer pairs for transgenic VvPIP2;4N (Supplemental Table S2) and nptII (Gambino et al, 2010) genes.…”

Section: Grapevine Transformation and Selectionmentioning

confidence: 99%

The Grapevine Root-Specific Aquaporin VvPIP2;4N Controls Root Hydraulic Conductance and Leaf Gas Exchange under Well-Watered Conditions But Not under Water Stress

et al. 2012

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We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;4N is the most expressed PIP2 gene in root. In situ hybridization confirmed root localization in the cortical parenchyma and close to the endodermis. We then constitutively overexpressed VvPIP2;4N in grape 'Brachetto', and in the resulting transgenic plants we analyzed (1) the expression of endogenous and transgenic VvPIP2;4N and of four other aquaporins, (2) whole-plant, root, and leaf ecophysiological parameters, and (3) leaf abscisic acid content. Expression of transgenic VvPIP2;4N inhibited neither the expression of the endogenous gene nor that of other PIP aquaporins in both root and leaf. Under well-watered conditions, transgenic plants showed higher stomatal conductance, gas exchange, and shoot growth. The expression level of VvPIP2;4N (endogenous + transgene) was inversely correlated to root hydraulic resistance. The leaf component of total plant hydraulic resistance was low and unaffected by overexpression of VvPIP2;4N. Upon water stress, the overexpression of VvPIP2;4N induced a surge in leaf abscisic acid content and a decrease in stomatal conductance and leaf gas exchange. Our results show that aquaporin-mediated modifications of root hydraulics play a substantial role in the regulation of water flow in well-watered grapevine plants, while they have a minor role upon drought, probably because other signals, such as abscisic acid, take over the control of water flow.

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“…Different morphological characteristics were the methodology used for selecting wild vines in the field. DNA from all samples was extracted, following the protocol of Thomas et al (1993). Primer sequences and DNA analysis were carried out, following the protocols described by Almadanim et al (2007) and Cunha et al (2009).…”

Section: S54mentioning

confidence: 99%

Genetic diversity in portuguese native Vitis vinifera L. ssp. vinifera and ssp. sylvestris

Cunha¹,

Santos²,

Brazão³

et al. 2010

CZECH J GENET PLANT

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To assess the different origins of Portuguese grapevine varieties, we used six nuclear and four chloroplastidal microsatellites as molecular markers, in order to compare the genetic structure of native wild-vines with native grapevine varieties. Both native subspecies have a great diversity, and a high interrelationship across the six nuclear microsatellites. Although identical numbers of alleles were found in each population, their distribution was different in the <I>vinifera </I>and <I>sylvestris </I>subspecies. Portuguese wild-vines have only chlorotypes A and B; A being the most frequent. The fifty-seven analysed Portuguese varieties have chlorotypes A, B, C, and D. The most frequent was the chlorotype A (75%), followed by D (21%). The results obtained reinforced the idea of Western Europe as having been one of the domestication centres for the grapevine, with contributions from the Eastern European gene pool. The observed genetic structure is a starting point from which to clarify the high number of native cultivars found in Portugal, and reinforces their probable origin in the Iberian Peninsula.

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Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification

Cited by 213 publications

References 28 publications

Ampelographic and Genetic Characterization of Grapevine Varieties (Vitis vinifera L.) of the ‘Mavroudia’ Group Cultivated in Greece

Ampelographic and Genetic Characterization of Grapevine Varieties (Vitis vinifera L.) of the ‘Mavroudia’ Group Cultivated in Greece

The Grapevine Root-Specific Aquaporin VvPIP2;4N Controls Root Hydraulic Conductance and Leaf Gas Exchange under Well-Watered Conditions But Not under Water Stress

Genetic diversity in portuguese native Vitis vinifera L. ssp. vinifera and ssp. sylvestris

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