Repetitive determinations of amylase, maltose, sucrose, and lactose by sample injection in closed flow-through systems

Nikolelis, Dimitrios P.; Mottola, Horacio A.

doi:10.1021/ac50034a024

Cited by 23 publications

(6 citation statements)

References 23 publications

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“…Their kinetic model provided suitable prediction of experimental results and computer calculations were inexpensive and fast. Incidentally, the same dual enzyme system was used analytically in soluble preparations (206). In a subsequent paper, Mercer and O'Driscoll (207) examined the model's use to predict the results of changes of relevant system parameters.…”

Section: Miscellaneous Kinetic Aspects Of Analytical Interestmentioning

confidence: 99%

Kinetic determinations and some kinetic aspects of analytical chemistry

Mottola¹,

Mark²

1984

Anal. Chem.

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received his B.A. degree from the University of Virginia In 1956 and his Ph.D. degree from Duke University in 1960. He was a postdoctoral research associate at the University of North Carolina (with C. N. Rellley) from 1960 to 1962 and at the California Institute of Technology (with F. C. Anson) from 1962 to 1963. He was a member of the staff of the Department of Chemistry at the University of Michigan from 1963 to 1970, Visiting Professor ol Chemistry at the Unlversite Libre de Bruxelles. 1970, and Joined the staff at the University of Cincinnati in 1970. He was the Department Head from 1976 to 1981. Hfe research Interests are In electrochemistry, surface chemistry, kinetic methods of analysis, environmental analytical problems, and instrumentation. In addition to research papers, he is the coauthor of the books "Kinetics in Analytical Chemistry", "Activated Carbon: Surface Chemistry and Adsorption from Solution", and "Simplified Circuit Analysis; Digital-Analog Logic". He Is also a coeditor of the monograph series "Computers In Chemistry and Instrumentation" and "Water Quality Handbook" and has been a member of the Editorial Board of Analytical Chemistry. Analytical Letters, Chemical Instrumentation, and Talanta.

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Section: Miscellaneous Kinetic Aspects Of Analytical Interestmentioning

confidence: 99%

Kinetic determinations and some kinetic aspects of analytical chemistry

Mottola¹,

Mark²

1984

Anal. Chem.

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“…However, it must be noted that air bubbles trapped at either electrode causes spurious readings. [6], Keller-Richter [7], and Bartscher [8] incremental methods have been discussed, there is little information in the literature on practical comparisons. The automated titrator described here has enabled hundreds *Correspondence to this author 194 of unbiased titration results to be printed out rapidly for the various incremental techniques.…”

Section: Effect Of Molybdatementioning

confidence: 99%

“…In this system the sample is introduced as a plug into a flowing stream via a valve or syringe, and mixing is accomplished by diffuon [2]. In contrast to CFA, the signal in FIA does not reach a steady state plateau, but gives sharp peaks [21. FIA has the advantage of a sampling rate commonly over 120 per hour, and as high as 300 per hour [7] or more [8,9]. The several applications of flow injection analysis have been reviewed by Betteridge [2].…”

Section: Introductionmentioning

confidence: 99%

Amperometric systems for thedetermination of oxidase enzymedependent reactions by continuous flowand flow injection analysis

Gulberg

Attiyat

Christian

1980

Journal of Analytical Methods in Chemistry

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“…The high cost of enzymes justifies attempts to regenerate and reuse them in the system. [7][8][9][10][11] Among the analytical methods, although spectrophotometry is the most commonly used in FIA, there appears to be little in the literature concerning cyclic FIA with spectrophotometric detection. To reuse chromogenic reagents effectively, a rapid regeneration system for them is desired under circulating flow conditions.…”

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Cyclic Flow-Injection Analysis for the Repetitive Determination of Zinc with 2-(5-Bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol and EDTA

et al. 2005

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Cyclic methods of analysis can lead to many advantages, such as low consumption of reagents, minimization of waste, high sample throughput, simple equipment, extended running hours and no contamination of the environment. Flow-injection analysis (FIA) is considered to be a most suitable technique for cyclic methods, i.e., the outlet from the flow-through cell is fed continuously back to the reservoir using a single-line flow system. On this cyclic FIA, the chemical reaction can be easily controlled by fixing the flow rate of a circulating reagent carrier solution and by the constant introduction of a sample solution to the carrier stream. Reproducible mixing of the sample and the reagents can be achieved within the closed flow system, resulting in a highly precise and reproducible signal response. Since the reaction products accumulate in the circulated flow system, a gradual built-up of the background signal produces a continuous baseline shift that limits the useful detection scale. Therefore, elimination of the accumulated reaction products and regeneration of the main reagent are required to maintain a long-running flow system. Some reports have been published concerning cyclic FIA using kinetic (reaction rate) methods 1-4 or the skillful application of electrolysis for removing metal ions. 5,6 Other reports include the incorporation of an immobilized enzyme column for its effective use. The high cost of enzymes justifies attempts to regenerate and reuse them in the system. [7][8][9][10][11] Among the analytical methods, although spectrophotometry is the most commonly used in FIA, there appears to be little in the literature concerning cyclic FIA with spectrophotometric detection. To reuse chromogenic reagents effectively, a rapid regeneration system for them is desired under circulating flow conditions.In a rapid communication, 12 we have proposed a new concept to eliminate the reaction products in a cyclic FIA system. The adoption of a complexing agent (inhibitor) together with a chromogenic reagent makes it possible to regenerate and reuse the main reagent repeatedly. To recognize the new concept and to further test its applicability, a cyclic FIA system has been proposed for the determination of zinc. In order to improve the manipulation and the sensitivity, a water-soluble pyridylazo derivative, 2-(5-bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol (5-Br-PAPS), 13-17 is used as a chromogenic reagent. Hayashibe et al. 18 have shown that 5-Br-PAPS is a selective and sensitive reagent for zinc (ε = 1.4 × 10 5 dm 3 mol -1 cm -1 , at 554 nm), and successfully applied it to the determination of zinc in serum using conventional FIA without any preconcentration or separation procedure. The aim of the work described here is a cyclic FIA system for the repetitive determination and/or monitoring of zinc using 5-Br-PAPS and EDTA by spectrophotometry. Experimental ReagentsAll chemicals used were of analytical-reagent grade, and water was purified in a Millipore Milli-Q system. A zinc standard solution...

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Repetitive determinations of amylase, maltose, sucrose, and lactose by sample injection in closed flow-through systems

Cited by 23 publications

References 23 publications

Kinetic determinations and some kinetic aspects of analytical chemistry

Kinetic determinations and some kinetic aspects of analytical chemistry

Amperometric systems for thedetermination of oxidase enzymedependent reactions by continuous flowand flow injection analysis

Cyclic Flow-Injection Analysis for the Repetitive Determination of Zinc with 2-(5-Bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol and EDTA

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