Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells

Porcherie, Adeline; Cunha, Patricia; Trotereau, Angélina; Roussel, Perrine; Gilbert, Florence; Rainard, Pascal; Germon, Pierre

doi:10.1186/1297-9716-43-14

Cited by 88 publications

(94 citation statements)

References 45 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The inherent capability of the innate system to respond to a vast number of pathogens is mediated by its ability to recognize highly conserved motifs shared by diverse pathogens, commonly referred to as PAMPs. It has been shown that a prompt response of the mammary after E. coli entry into the lumen of the gland is required to control the infection, which means that early detection of bacteria are of prime of importance (Bannerman et al, 2004;Porcherie et al, 2012).…”

Section: Mastitis Caused By Escherichia Colimentioning

confidence: 99%

“…Science Publications AJI role in the production of cytokines (Riollet et al, 2000;Strandberg et al, 2005;Griesbeck-Zilch et al, 2008;Guntler et al, 2011;Porcherie et al, 2012). The upregulation of cytokine production is a key component of the host innate immune response to infection (Bannerman et al, 2004;Schukken et al, 2011).…”

Section: The Importance Of These Cells Is Demonstrated By Theirmentioning

confidence: 99%

See 1 more Smart Citation

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

Souza¹

2012

American Journal of Immunology

View full text Add to dashboard Cite

Mastitis is the most costly disease for dairy farmers and industry, which are mainly caused by the entry of bacteria to the teat canal. Shortly after the entry of the invading bacteria, the innate immunity recognizes the invading pathogen through pattern recognition receptors and initiates the inflammatory response necessary to eliminate the invading bacteria. This initial inflammatory response releases cytokines and chemoattractants for the rapid and massive influx of neutrophils from the blood to the site of infection which form the first line of cellular defense against bacteria This article reviewed the role of the most recent knowledge regarding the innate immunity in bovine mastitis focusing in the two major mastitis pathogens: Escherichia coli and Staphylococcus aureus the S. aureus appears to mostly circumvent the host immune response, as the Toll-Like Receptors (TLRs) signaling pathways. The Intramammary Infections (IMIs) by this bacteria result in a very moderate host response with minimal observable innate immune response, which are related to well-known ability to this pathogen to establish chronic IMI. Otherwise, E. coli elicits a strong and earlier response, mainly through TLR4, that is associated with the severity of the mastitis and the clinical manifestation commonly observed in dairy cows infected with this pathogen. Suboptimal and dysfunctional mammary defenses may contribute to the development of severe acute inflammation or chronic mastitis that adversely affects the milk production and quality. Thus, a better understanding of mastitis pathogen interaction to the host may be useful for future control of mastitis.

show abstract

Section: Mastitis Caused By Escherichia Colimentioning

confidence: 99%

Section: The Importance Of These Cells Is Demonstrated By Theirmentioning

confidence: 99%

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

Souza¹

2012

American Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…The recognition of E. coli by the MG has been investigated, mainly in the cow. Recently, we and others characterized the repertoire of bacterial agonists sensed by the udder and bovine mammary epithelial cells (MECs) during E. coli mastitis, showing that the MG is equipped to sense Gram-negative bacteria through different pattern recognition receptors such as TLRs and the nucleotide oligomerization domain receptors NOD1 and NOD2 (9,10). The cytokine profile associated with E. coli mastitis has also been partially described by using experimentally induced mastitis in the cow.…”

mentioning

confidence: 99%

IL-17A Is an Important Effector of the Immune Response of the Mammary Gland to Escherichia coli Infection

Porcherie

Gilbert

Germon

et al. 2016

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

The cytokine IL-17A has been shown to play critical roles in host defense against bacterial and fungal infections at different epithelial sites, but its role in the defense of the mammary gland (MG) has seldom been investigated, although infections of the MG constitute the main pathology afflicting dairy cows. In this study, we showed that IL-17A contributes to the defense of the MG against Escherichia coli infection by using a mouse mastitis model. After inoculation of the MG with a mastitis-causing E. coli strain, the bacterial load increased rapidly, triggering an intense influx of leukocytes into mammary tissue and increased concentrations of IL-6, IL-22, TNF-α, and IL-10. Neutrophils were the first cells that migrated intensely to the mammary tissue, in line with an early production of CXCL2. Depletion of neutrophils induced an increased mammary bacterial load. There was a significant increase of IL-17–containing CD4+ αβ T lymphocyte numbers in infected glands. Depletion of IL-17A correlated with an increased bacterial colonization and IL-10 production. Intramammary infusion of IL-17A at the onset of infection was associated with markedly decreased bacterial numbers, decreased IL-10 production, and increased neutrophil recruitment. Depletion of CD25+ regulatory T cells correlated with a decreased production of IL-10 and a reduced bacterial load. These results indicate that IL-17A is an important effector of MG immunity to E. coli and suggest that an early increased local production of IL-17A would improve the outcome of infection. These findings point to a new lead to the development of vaccines against mastitis.

show abstract

“…BMECs are poised to respond quickly to pathogen invasion by binding to pathogen-associated molecular pattern molecules via the activation of various pattern recognition receptors (PRRs) (16,17). In general, the binding of a pathogen to a Toll-like receptor (TLR) or nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) activates the MAPK and NF-B signaling cascades, leading to upregulate expression of various proinflammatory cytokines, chemokines, and antimicrobial peptides, thereby triggering inflammation and host immune responses (18).…”

mentioning

confidence: 99%

Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation

Liu

Yang

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. We explored the effects of the probiotic Lactobacillus rhamnosus GR-1 on ameliorating E. coli-induced inflammation and cell damage in primary bovine mammary epithelial cells (BMECs). Increased Toll-like receptor 4 (TLR4), NOD1, and NOD2 mRNA expression was observed following E. coli challenge, but this increase was attenuated by L. rhamnosus GR-1 pretreatment. Immunofluorescence and Western blot analyses revealed that L. rhamnosus GR-1 pretreatment decreased the E. coli-induced increases in the expression of the NOD-like receptor family member pyrin domain-containing protein 3 (NLRP3) and the serine protease caspase 1. However, expression of the adaptor protein apoptosis-associated speck-like protein (ASC, encoded by the Pycard gene) was decreased during E. coli infection, even with L. rhamnosus GR-1 pretreatment. Pretreatment with L. rhamnosus GR-1 counteracted the E. coli-induced increases in interleukin-1␤ (IL-1␤), -6, -8, and -18 and tumor necrosis factor alpha mRNA expression but upregulated IL-10 mRNA expression. Our data indicate that L. rhamnosus GR-1 reduces the adhesion of E. coli to BMECs, subsequently ameliorating E. coli-induced disruption of cellular morphology and ultrastructure and limiting detrimental inflammatory responses, partly via promoting TLR2 and NOD1 synergism and attenuating ASC-independent NLRP3 inflammasome activation. Although the residual pathogenic activity of L. rhamnosus, the dosage regimen, and the means of probiotic supplementation in cattle remain undefined, our data enhance our understanding of the mechanism of action of this candidate probiotic, allowing for development of specific probiotic-based therapies and strategies for preventing pathogenic infection of the bovine mammary gland. Mastitis affects cows in all regions of the world and can cause a decrease in milk production and quality, resulting in a major economic burden to the dairy industry (1). Escherichia coli is by far the most common cause of mastitis, since it is isolated in more than 80% of cases of coliform mastitis (2). Antibiotic therapy frequently leaves residues in milk, potentially facilitating the development of antibiotic resistance. Probiotics represent a novel alternative to antibiotics for controlling pathogen infections.Lactobacillus rhamnosus GR-1 is a probiotic bacterium isolated from the female urethra, and oral ingestion of L. rhamnosus GR-1 and L. fermentum RC-14 reduces vaginal colonization by pathogenic bacteria and yeasts and maintains urogenital health in women (3). Pretreatment of pregnant CD-1 mice with L. rhamnosus GR-1 culture supernatant decreases lipopolysaccharide (LPS)-induced production of various cytokines and chemokines (4). Lactobacillus rhamnosus GR-1 suppresses expression of nuclear factor (NF)-B-related inflammatory genes and activates alternate mitogen-activated protein kinase (MAPK) and activator protein 1 pathways to recruit host defense factors...

show abstract

Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells

Cited by 88 publications

References 45 publications

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

IL-17A Is an Important Effector of the Immune Response of the Mammary Gland to Escherichia coli Infection

Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation

Contact Info

Product

Resources

About