Repelling and ordering: the influence of poly(ethylene glycol) on protein adsorption

Bernhard, Christoph; Roeters, Steven J.; Franz, Johannes; Weidner, Tobias; Bonn, Mischa; Gonella, Grazia

doi:10.1039/c7cp05445a

Cited by 39 publications

(38 citation statements)

References 54 publications

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“…5°, in agreement with previously published results [14,15]. After PEG immobilization, the contact angle decreased significantly (58 ± 1°), as expected due to the well-known hydrophilic character of PEG [16]. After peptide immobilization, there was only a slight increase in the water contact angle for both SH-AMP (61 ± 1°) and AMP-SH (61 ± 2°).…”

Section: Introductionsupporting

confidence: 91%

AMP–Chitosan Coating with Bactericidal Activity in the Presence of Human Plasma Proteins

et al. 2020

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Antibiotic resistance is increasing and new strategies are needed to fight infection. Advanced materials are promising tools that can be combined with innovative alternatives to conventional antibiotics to allow more targeted and efficient treatment. In this work, we explored the activity against Staphylococcus epidermidis (S. epidermidis) of the α-helical antimicrobial peptide (AMP) MSI-78(4-20) (KFLKKAKKFGKAFVKIL) when covalently bound to a chitosan coating. The AMP MSI-78(4-20) (17 mer) is an improved version of its parent MSI-78 (22 mer; commercially known as Pexiganan), a cost-effective short AMP, which was demonstrated to be as effective as MSI-78 and less toxic to eukaryotic cells. An MSI-78(4-20)–chitosan coating could be applied in several infection scenarios, ranging from bone implants to wound dressings, as chitosan possesses osteoconductive and hemostatic properties. Cysteine-modified MSI-78(4-20) was covalently immobilized onto the chitosan coating through a succinimidyl-[(N-maleimidopropionamido)-octaethyleneglycol] ester (SM(PEG)8), a heterobifuncional crosslinker, with N-hydroxysuccinimide (NHS) ester and maleimide groups, by its N- and C- termini. The MSI-78(4-20)–chitosan coating demonstrated bactericidal properties independently of the tethering site and an improved performance in the presence of plasma proteins, which mimics conditions that will be encountered in vivo. This AMP–chitosan coating has therefore great potential for applications in medical devices such as implants or even wound dressings.

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Section: Introductionsupporting

confidence: 91%

AMP–Chitosan Coating with Bactericidal Activity in the Presence of Human Plasma Proteins

et al. 2020

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“…Protein-repellent properties of PEG grafted on surfaces are influenced by PEG chain length, 18 density, and environment temperature, 19,20 although the amount of adsorbed proteins is not always a monotonic function of these parameters. 21 The use of PEG as a stealth agent also has some drawbacks, such as its non-biodegradability, immunogenicity, 22 and its accumulation in membrane-bound organelles. 23 An alternative to PEG is represented by ligands terminated by zwitterionic moieties, which further reduce nonspecific protein adsorption.…”

Section: Introductionmentioning

confidence: 99%

Role of Ligand Conformation on Nanoparticle–Protein Interactions

2019

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Engineered biomedical nanoparticles (NPs) administered via intravenous routes are prone to associate to serum proteins. The protein corona can mask the NP surface functionalization and hamper the delivery of the NP to its biological target. The design of corona-free NPs relies on our understanding of the chemical-physical features of the NP surface driving the interaction with serum proteins. Here, we address, by computational means, the interaction between human serum albumin (HSA) and a prototypical monolayer-protected Au nanoparticle. We show that both the chemical composition (charge, hydrophobicity) and the conformational preferences of the ligands decorating the NP surface affect the NP propensity to bind HSA.

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“…Poly(ethylene) glycol (PEG)-based hydrogels have been investigated extensively for a range of biomedical applications due to their excellent biocompatibility, non-immunogenicity and resistance to protein adhesion [1,2,3,4]. The main areas of application include devices for wound healing, tissue-engineering constructs and drug-delivery systems within the pharmaceutical industry [5,6].…”

Section: Introductionmentioning

confidence: 99%

Controlling Fluid Diffusion and Release through Mixed-Molecular-Weight Poly(ethylene) Glycol Diacrylate (PEGDA) Hydrogels

2019

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Due to their inherent ability to swell in the presence of aqueous solutions, hydrogels offer a means for the delivery of therapeutic agents in a range of applications. In the context of designing functional tissue-engineering scaffolds, their role in providing for the diffusion of nutrients to cells is of specific interest. In particular, the facility to provide such nutrients over a prolonged period within the core of a 3D scaffold is a critical consideration for the prevention of cell death and associated tissue-scaffold failure. The work reported here seeks to address this issue via fabrication of hybrid 3D scaffolds with a component fabricated from mixed-molecular-weight hydrogel formulations capable of storing and releasing nutrient solutions over a predetermined time period. To this end, poly(ethylene) glycol diacrylate hydrogel blends comprising mixtures of PEGDA-575 Mw and PEGDA-2000 Mw were prepared via UV polymerization. The effects of addition of the higher-molecular-weight component and the associated photoinitiator concentration on mesh size and corresponding fluid permeability have been investigated by diffusion and release measurements using a Theophylline as an aqueous nutrient model solution. Fluid permeability across the hydrogel films has also been determined using a Rhodamine B solution and associated fluorescence measurements. The results indicate that addition of PEGDA-2000 Mw to PEGDA-575 Mw coupled with the use of a specific photoinitiator concentration provides a means to change mesh size in a hydrogel network while still retaining an overall microporous material structure. The range of mesh sizes created and their distribution in a 3D construct provides for the conditions required for a more prolonged nutrient release profile for tissue-engineering applications.

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Repelling and ordering: the influence of poly(ethylene glycol) on protein adsorption

Cited by 39 publications

References 54 publications

AMP–Chitosan Coating with Bactericidal Activity in the Presence of Human Plasma Proteins

AMP–Chitosan Coating with Bactericidal Activity in the Presence of Human Plasma Proteins

Role of Ligand Conformation on Nanoparticle–Protein Interactions

Controlling Fluid Diffusion and Release through Mixed-Molecular-Weight Poly(ethylene) Glycol Diacrylate (PEGDA) Hydrogels

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