Repeated Morphine Administration Increases TRPV1 mRNA Expression and Autoradiographic Binding at Supraspinal Sites in the Pain Pathway

Abstract

Repeated morphine administration induces tolerance to its analgesic effects. A previous study reported that repeated morphine treatment activates transient receptor potential vanilloid type 1 (TRPV1) expression in the sciatic nerve, dorsal root ganglion, and spinal cord, contributing to morphine tolerance. In the present study, we analyzed TRPV1 expression and binding sites in supraspinal pain pathways in morphine-tolerant mice. The TRPV1 mRNA levels and binding sites were remarkably increased in the cortex and thalamus of these animals. Our data provide additional insights into the effects of morphine on TRPV1 in the brain and suggest that changes in the expression of, and binding to TRPV1 in the brain are involved in morphine tolerance.

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“…Heat and protons can activate TRPV1 channels on their own. Furthermore, TRPV1 channels are upregulated in the sciatic nerve, dorsal root ganglia, spinal cord [ 63 ], sensory cortex, and thalamus [ 64 ] following chronic morphine treatment. The administration of TRPV1 channel antagonists in the nucleus accumbens significantly reduces morphine self-administration in rats [ 65 ].…”