Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles

Park, Eun-Jung; Bae, Eunjoo; Yi, Jongheop; Kim, Younghun; Choi, Kyunghee; Lee, Sangeun; Yoon, Junheon; Lee, Byung Chun; Park, Kwangsik

doi:10.1016/j.etap.2010.05.004

Cited by 490 publications

(318 citation statements)

References 26 publications

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“…However, smaller Ag NPs were found distributed in several tissues (brain, lung, liver, kidney and testis), while larger NPs were not. The same group has demonstrated that repeated doses of 42 nm uncoated Ag NPs were able to induce significant inflammation, involving increase of the cytokines produced, B cells distribution and tissue infiltration of inflammatory cells [16]. Distribution and accumulation of three different Ag NPs sizes (20,80 and 110 nm), administered by intravenous injection in rats once daily for 5 days, were found for the smallest Ag NPs (20 nm) in liver, kidneys and spleen, and for the larger Ag NPs (80 and 110 nm) in spleen, liver and lung [17].…”

Section: Introductionmentioning

confidence: 99%

“…In vivo studies report that 1 mg/kg of Ag NPs (22,42,71 and 323 nm) administered by ingestion to mice over 14 days produced no difference in the body or organs weight between the treated and the control groups [16]. However, smaller Ag NPs were found distributed in several tissues (brain, lung, liver, kidney and testis), while larger NPs were not.…”

Section: Introductionmentioning

confidence: 99%

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Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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Silver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnologybased products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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show abstract

“…Thus, recent findings suggest that there is a relationship between AgNPs and the immune system, however, there are almost no reports on the AgNP immunomodulation of tumor-bearing mice. Although there are several reports on the toxicity of AgNPs, [21][22][23][24][25] there is a paucity of toxicity studies on the AgNP dose-response in mice. Hence, the present investigation was designed to determine the effects of AgNPs at different doses in mice and to study their effects on murine fibrosarcoma.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

et al. 2015

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The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles (AgNPs) have been reported as one type of potent therapeutic nanoparticles. The present study is aimed to determine the effect of AgNPs in arresting the growth of a murine fibrosarcoma by a reductive mechanism. Initially, a bioavailability study showed that mouse serum albumin (MSA)-coated AgNPs have enhanced uptake; therefore, toxicity studies of AgNP-MSA at 10 different doses (1-10 mg/kg b.w.) were performed in LACA mice by measuring the complete blood count, lipid profile and histological parameters. The complete blood count, lipid profile and histological parameter results showed that the doses from 2 to 8 mg (IC 50 : 6.15 mg/kg b.w.) sequentially increased the count of leukocytes, lymphocytes and granulocytes, whereas the 9-and 10-mg doses showed conclusive toxicity. In an antitumor study, the incidence and size of fibrosarcoma were reduced or delayed when murine fibrosarcoma groups were treated by AgNP-MSA. Transmission electron micrographs showed that considerable uptake of AgNP-MSA by the sentinel immune cells associated with tumor tissue and a morphologically buckled structure of the immune cells containing AgNP-MSA. Because the toxicity studies revealed a relationship between AgNPs and immune function, the protumorigenic cytokines TNF-a, IL-6 and IL-1b were also assayed in AgNP-MSA-treated and non-treated fibrosarcoma groups, and these cytokines were found to be downregulated after treatment with AgNP-MSA. Cellular & Molecular Immunology

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“…Recent evidence from a 28-day oral study in rats with post-exposure follow-up over 1 and 8 weeks suggests that clearance of silver from testis is very slow, regardless of whether silver or nanosilver was applied (Van der Zande et al 2012). Also for mice, Park et al (2010) reported similar or higher organ levels of silver in testes compared to liver following repeated oral exposure to 1 mg/kg bw/d Ag-NPs with a mean size of 22 and 42 nm, while no silver was detectable in testes for 71 and 323 nm sized particles.…”

Section: In Vivo Reproductive and Developmental Toxicitymentioning

confidence: 99%

“…The differences particularly concern particle size and coating. It was pointed out by Park et al (2010) that "most data on the toxicity of Ag-NPs have been generated using nanoparticles modified with detergents to prevent agglomeration, which may alter their toxicities". Detergents or coating material may not always have been identified in the studies.…”

Section: Genotoxicitymentioning

confidence: 99%

Potential risks and benefits of nanotechnology: perceptions of risk in sunscreens

Wright

2016

Medical Journal of Australia

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Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles

Cited by 490 publications

References 26 publications

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

Potential risks and benefits of nanotechnology: perceptions of risk in sunscreens

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