Repeated administration of different hormonal preparations for artificial propagation and their effects on reproduction, survival and blood biochemistry profiles of female tench (Tinca tinca L.)

Abstract

ABSTRACT:The aim of the study was to compare physiological statuses of stripped and not stripped tench females with ovulation induced by GnRHa or carp pituitary extract (CPE). The comparison was based on selected biochemical stress indices (cortisol, glucose), condition (TP, TGA), and cell membrane characteristics (ALT, AST, CK). In the first and in the second year, 37 and 29 tench females were monitored, respectively. No differences in the values of biochemical parameters in either period were found between the two groups of stripped female tench whose ovulation was induced by GnRHa and CPE, respectively. No significant differences in TP, TGA, ALT, AST and CK values were found between the groups of stripped and not stripped females with ovulation induced by GnRHa or CPE. Different values were found in stress indices, specifically in glucose concentrations. A significant increase (P < 0.01) in glucose concentrations was found in tench females immediately after stripping and, in not stripped females, about 48 hrs after ovulation induction. Both artificial stripping and the inability to release eggs are important stress factors for female tench.

Induction of final oocyte maturation in Cyprinidae fish by hypothalamic factors: a review

“…From the viewpoint of labour reduction and mainly for elimination of stress of broodstock, however, it is much more advantageous to administer one combined dose of GnRHa with DI (Kouril et al, 1999), which is also one of the advantages of GnRHa preparations, as compared with the carp pituitary. Based on the published literature we can distinguish four main sites of hormone administration: (a) intraperitoneal injection (IP) -into the abdomen wall 2 cm above the ventral fin (Kouril et al, 2006), (b) intramuscular injection (IM) -penetration of the dorsal muscle 2-3 cm below dorsal fin beginning (Kouril et al, 2007), (c) pericardial cavity injection (IPP) -into the pericardial cavity (Kouril et al, 1986), (d) intravenous injection (IV) -puncture of the caudal vein at the level of the anal fin (Mikolajczyk et al, 2003). Unlike in IP and IPP, in IM administration an effluence of the injected preparation from tissue can occur, which has a negative impact on successful FOM.…”

“…The inhibitory effect of DA on LH secretion changes over the course of the reproductive cycle, with the maximum DA inhibition occurring during the final stages of gametogenesis. This feature is utilised in aquaculture of Cyprinidae by using dopamine antagonists in ovulation-inducing therapies, e.g., domperidon, pimozide, reserpin, metoclopramide, haloperidol, isofloxythepin Brzuska, 1999;Kouril et al, 2006Kouril et al, , 2007.…”

“…Substitution of amino acids also modified polarity and tertiary structure of the GnRHa, which results in an improvement receptor binding affinity (Zohar and Mylonas, 2001). The unsatisfactory potency of natural GnRH peptides was improved by synthesising a superactive GnRHa, which is able to induce a significant increase in LH levels even at centuple smaller doses than with the use of natural GnRH forms (Table 1), (Kouril et al, 1986(Kouril et al, , 2007. The range of effective doses of GnRHa varies from 5-100 μg/kg and in the case of DI from 5-20 mg/kg of effective matter (Kouril et al, 1986;Drori et al, 1994;Brzuska, 1999;Szabo et al, 2002;Glasser et al, 2004;Mikolajczyk et al, 2004;Rutaisire and Booth, 2004;Kucharcyzk et al, 2005;Heyrati et al, 2007 Due to the strong dopaminergic inhibition of LH secretion, typical for the family Cyprinidae, a majority of trials with ovulation induction using only GnRHa failed (Weil et al, 1980;Sokolowska et al, 1984).…”

Hormonal induction of ovulation in pikeperch (Sander lucioperca L.) using human chorionic gonadotropin (hCG) and mammalian GnRH analogue

“…Maturity stage of the oocytes from each female was determined according to the method described by Kucharczyk et al (2007). Then, females were intramuscularly under dorsal fin injected with the Chorulon containing hCG (Intervet, the Netherlands) or the Supergestran containing mGnRHa (Lecirelin (D-Tle6) GnRHm ProNHEt) (FERRING LÉ Č IVA, Czech Republic) as described by (Policar et al 2008;Kouril et al 2007). The biological characteristics of females in different hormonally treated groups as well as doses of injection are presented in Table 1.…”

Eggs Production of Grouper Hybrid for Supporting Hatchery Business

“…Penyuntikan merupakan cara yang sederhana, dapat dilakukan secara intramuscular (otot daging) yaitu penyuntikan pada bagian otot bagian dorsal 2 -3 cm di bawah sirip dorsal pertama (Kouril et al, 2007).…”