Repeatability and Relationship of Incidence and Severity Measures of Scab of Wheat Caused by <i>Fusarium graminearum</i> in Inoculated Nurseries

Groth, J. V.; Ozmon, E. A.; Busch, R. H.

doi:10.1094/pdis.1999.83.11.1033

Cited by 65 publications

(62 citation statements)

References 5 publications

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“…Despite the significant genotype-by-year interaction for FHB-related traits, broad-sense heritabilities were high (H=0.75-0.84), indicating a reproducible assessment of the FHB resistance level. FHB incidence and FHB severity were highly correlated, indicating that low FHB incidence and low FHB severity are under similar genetic control, which is in agreement with Groth et al (1999). However, FHB spread showed moderate to low association with other FHB-related traits.…”

Section: Fhb Resistance Assessmentsupporting

confidence: 81%

Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana

Steiner¹,

Lemmens²,

Griesser³

et al. 2004

Theor Appl Genet

194

196

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Fusarium head blight (FHB) is a destructive disease of wheat. The objective of this study was to characterise the FHB resistance of the Brazilian spring wheat cultivar Frontana through molecular mapping. A population of 210 doubled-haploid lines from a cross of Frontana (partially resistant) and Remus (susceptible) was evaluated for FHB resistance during three seasons. Spray and single-spikelet inoculations were applied. The severity, incidence and spread of the disease were assessed by visual scoring. The population was genotyped with 566 DNA markers. The major QTL effect associated with FHB resistance mapped to chromosome 3A near the centromere, explaining 16% of the phenotypic variation for disease severity over 3 years. The most likely position is in the Xgwm720-Xdupw227 interval. The genomic region on 3A was significantly associated with FHB severity and incidence in all years evaluated, but not with FHB spread, indicating the prominent contribution of this QTL to resistance against initial infection. The map interval Xgwm129-Xbarc197 on chromosome 5A also showed consistent association with FHB severity and accounted for 9% of the phenotypic variation. In addition, smaller effects for FHB severity were identified on chromosomes 1B, 2A, 2B, 4B, 5A and 6B in single years. Individual QTLs for resistance to FHB spread accounted for less than 10% of the variation in trait expression. The present study indicates that FHB resistance of Frontana primarily inhibits fungal penetration (type I resistance), but has a minor effect on fungal spread after infection (type II resistance).

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Section: Fhb Resistance Assessmentsupporting

confidence: 81%

Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana

Steiner¹,

Lemmens²,

Griesser³

et al. 2004

Theor Appl Genet

194

196

View full text Add to dashboard Cite

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“…The fungus was maintained routinely on 4 % potato dextrose agar (PDA) plates and the long-term stock was kept at 280 uC as a spore suspension in 15 % glycerol. For inoculum production, strain PH-1 and mutants were cultured on cornmeal agar plates for at least 7 days before making the conidial suspension (Groth et al, 1999). For growth assays, the wild-type strain and mutants were cultured at 24 uC on Czapek-Dox minimal medium (0?2 % NaNO 3 , 0?05 % KCl, 0?1 % K 2 HPO 4 , 0?05 % MgSO 4 .7H 2 O and 0?001 % FeSO 4 .7H 2 O) supplemented with 2 % agar (for plates only) and substrate as required.…”

Section: Methodsmentioning

confidence: 99%

A secreted lipase encoded by LIP1 is necessary for efficient use of saturated triglyceride lipids in Fusarium graminearum

et al. 2005

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A triglyceride lipase gene LIP1 was identified in the genome of Fusarium graminearum strain PH-1. The predicted protein encoded by LIP1 contains 591 amino acid residues with a putative N-terminal signal peptide and shows 57 and 40-44 % identity to a Botrytis cinerea lipase and five Candida rugosa lipases, respectively. Yeast cells overexpressing LIP1 showed lipolytic activity against a broad range of triglyceride substrates. Northern blot analyses revealed that expression of LIP1 was activated in planta during the fungal infection process. LIP1 expression was strongly induced in minimal medium supplemented with wheatgerm oil, but only weakly induced by olive oil and triolein. In contrast, supplementation with other carbon sources, including glucose, sucrose, apple pectin and wheat cell-wall material, did not induce LIP1 expression. Saturated fatty acids were the strongest inducers for LIP1 expression and this induction was suppressed proportionally by the presence of the unsaturated fatty acid. To determine the potential function of LIP1, gene replacement was conducted on strain PH-1. When compared with wild-type PH-1, DLIP1 mutants showed greatly reduced lipolytic activities at the early stage of incubation on minimal medium supplemented with either saturated or unsaturated lipid as the substrate, indicating that LIP1 encodes a secreted lipase for exogenous lipid hydrolysis. Moreover, the DLIP1 mutants exhibited growth deficiency on both liquid and solid minimal media supplemented with the saturated triglyceride tristearin as the sole carbon source, suggesting that LIP1 is required for utilization of this substance. Despite these differences, no variation in disease symptoms between the DLIP1 mutants and the wild-type strain was observed on susceptible cereal hosts.

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“…Plot disease severity for a population of approximately 200 spikes per plot was estimated for both incidence (percentage of infected spikes) and severity (percentage of infected spikelets of the diseased spikes). An FHB index (incidence × severity)/100 was calculated to assess plot severity (Groth et al 1999).…”

Section: Field Trialmentioning

confidence: 99%

Study of metabolite profiles in winter wheat cultivars induced by Fusarium infection

Stuper‐Szablewska

Kurasiak-Popowska

Perkowski

2016

Cereal Research Communications

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The aim of this study was to determine the profile of fungal metabolites and antioxidant substances from plants on a representative population of wheat cultivars grown in Poland. Tests were conducted on grain of 23 wheat cultivars in three experimental variants: natural infection (control), chemical crop protection, and artificial inoculation with the fungi F. graminearum and F. culmorum. Grains were analysed in terms of infection rate, mycotoxin, ergosterol, free and bound phenolic acid content, as well as antioxidant capacity. Calculated Fusarium head blight index (FHB index) ranged from 0% to 24% in the control, from 0% to 95% after inoculation and from 0 to 3% following chemical crop protection treatments. The highest concentration of ergosterol was detected in seeds from inoculated plots, with a mean value of 26.69 mg/kg. The highest mean concentration calculated for total toxin content was recorded after inoculation (7.833 mg/kg). The mean value for ferulic acid for inoculated samples resulted 2574 mg/kg, while for chemically protected samples 1158 mg/kg was obtained.Based on of discriminant analysis genotypes can be separated for inoculation 100%, for chemical crop protection 95%, and for natural infection 90%. Amongst 12 analysed phenolic acids the highest discrimination power was found for gallic acid, 4-hydroxybenzoic acid and vanillic acid.

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Repeatability and Relationship of Incidence and Severity Measures of Scab of Wheat Caused by Fusarium graminearum in Inoculated Nurseries

Cited by 65 publications

References 5 publications

Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana

Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana

A secreted lipase encoded by LIP1 is necessary for efficient use of saturated triglyceride lipids in Fusarium graminearum

Study of metabolite profiles in winter wheat cultivars induced by Fusarium infection

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