Repairing Rabbit Radius Bone Defects with Simvastatin Compound Biological Bone

Abstract

Objective: This study aimed to investigate the feasibility of repairing rabbit radius bone defects with simvastatin compound biological bone. Methods: Simvastatin biological bone material was prepared, and osteoblasts were cultured. A total of 42 New Zealand white rabbits were randomly divided into four groups, and a bone defect with a length of 15 mm was created at the middle part of the radial shaft of both limbs in each rabbit, thereby establishing a bone defect model. The grafts in group A were biological bones of osteoblasts combined with simvastatin; the grafts in group B were biological bones of simvastatin; the grafts in group C were biological compound bones of osteoblasts; and the grafts in group D were simple biological bones. In each group, four animals were randomly sacrificed at the sixth and twelfth week after surgery, and specimens were collected for gross observation, X-ray examination, histological observation, and biomechanical testing. In each group, two animals were randomly sacrificed at the twelfth week after surgery; a three-point bending test was performed using a biomechanical testing machine, and the results were compared with those of a normal radius. Results: The X-ray and histological examinations at 6 and 12 weeks after surgery revealed that the osteogenesis ability of the simvastatin biological bone and osteoblast-simvastatin biological bone was better than that of the osteoblast biological bone and simple biological bone, which was superior in group A and group B to group C and group D. The results of the biomechanical examination revealed that the maximum stress of the normal radius was significantly higher than that of the experimental groups. Among the experimental groups, the difference between group A and group B was not statistically significant, and the maximum stress was higher in groups A and B than in groups C and D. Conclusion: Simvastatin biological bone material can promote the repair of rabbit radius defects and increase the quality of bone healing.