REPAIRING OF RABBIT SKULL DEFECT BY TGF-β1-INCORPORATED COLLAGEN SPONGES OF DIFFERENT THICKNESS

Ueda, Hiroki R.; Nakamura, Tatsu O.; Tabata, Yasuhiko; Shimizu, Yasuhiko

doi:10.4015/s101623720300002x

Cited by 2 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Related to a method described by Ueda, the resulting sponges were placed in a test tube, evacuated to 9 ± 3 mbar and subjected to 145 ± 5°C for 6 h 24. The obtained scaffolds were stored for at least 3 days in isopropanol and dried under reduced pressure prior to following experiments.…”

Section: Methodsmentioning

confidence: 99%

Fabrication and characterization of a biomimetic composite scaffold for bone defect repair

Nitzsche

Lochmann

Metz

et al. 2010

J Biomedical Materials Res

View full text Add to dashboard Cite

For successful bone tissue engineering, scaffolds with tailored properties are a basic requirement. The combination of different available materials not only appears to be desirable but also very challenging. In this study, a composite material consisting of hydroxyapatite and collagen was produced by a biomimetic precipitation method and characterized by X-ray diffraction (XRD) and thermogravimetry (TGA). Subsequently, a suspension-quick-freezing and lyophilization method was used to incorporate the hydroxyapatite into a polymeric matrix consisting of collagen and chitosan. Before physicochemical characterization, the highly porous scaffolds were consolidated by a dehydrothermal treatment (DHT). The main attention was focused on the particle size of hydroxyapatite, which should be in the nanometer range. This is relevant to achieve a homogeneous resorption of the material by osteoclasts. Small-angle X-ray scattering (SAXS), atomic force microscopy (AFM), and environmental scanning electron microscopy (ESEM) were used to evaluate the outcome. The results suggest a successful polymeric embedding of nanoscaled hydroxyapatite particles into the matrix of the spongy construct. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

show abstract

Section: Methodsmentioning

confidence: 99%

Fabrication and characterization of a biomimetic composite scaffold for bone defect repair

Nitzsche

Lochmann

Metz

et al. 2010

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

Cationized gelatin hydrogels mixed with plasmid DNA induce stronger and more sustained gene expression than atelocollagen at calvarial bone defectsin vivo

Komatsu

Shibata

Shimada

et al. 2016

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

Gene transduction of exogenous factors at local sites in vivo is a promising approach to promote regeneration of tissue defects owing to its simplicity and capacity for expression of a variety of genes. Gene transduction by viral vectors is highly efficient; however, there are safety concerns associated with viruses. As a method for nonviral gene transduction, plasmid DNA delivery is safer and simpler, but requires an efficient carrier substance. Here, we aimed to develop a simple, efficient method for bone regeneration by gene transduction and to identify optimal conditions for plasmid DNA delivery at bone defect sites. We focused on carrier substances and compared the efficiencies of two collagen derivatives, atelocollagen, and gelatin hydrogel, as substrates for plasmid DNA delivery in vivo. To assess the efficiencies of these substrates, we examined exogenous expression of green fluorescence protein (GFP) by fluorescence microscopy, polymerase chain reaction, and immunohistochemistry. GFP expression at the bone defect site was higher when gelatin hydrogel was used as a substrate to deliver plasmids than when atelocollagen was used. Moreover, the gelatin hydrogel was almost completely absorbed at the defect site, whereas some atelocollagen remained. When a plasmid harboring bone morphogenic protein 2 was delivered with the substrate to bony defect sites, more new bone formation was observed in the gelatin group than in the atelocollagen group. These results suggested that the gelatin hydrogel was more efficient than atelocollagen as a substrate for local gene delivery and may be a superior material for induction of bone regeneration.

show abstract

REPAIRING OF RABBIT SKULL DEFECT BY TGF-β1-INCORPORATED COLLAGEN SPONGES OF DIFFERENT THICKNESS

Cited by 2 publications

References 30 publications

Fabrication and characterization of a biomimetic composite scaffold for bone defect repair

Fabrication and characterization of a biomimetic composite scaffold for bone defect repair

Cationized gelatin hydrogels mixed with plasmid DNA induce stronger and more sustained gene expression than atelocollagen at calvarial bone defectsin vivo

Contact Info

Product

Resources

About