Repairing Collagen in Dentin Carious Lesions. Influence of Sealing the Material: A Morphometric Study

Abstract

Objective: The aim of the present work was to morphometrically evaluate collagen in carious lesions sealed with calcium hydroxide, adhesive systems, glass ionomer cement, and an antibacterial cement. Study design: Samples of infected and affected dentin were stained with Sirius Red (SR). The areas intensively stained with SR were delimited, and the percentage of these areas was measured by blind calibrated examiners. The mean results were subjected to the Kruskal-Wallis test. Results: The affected dentin sealed with Ca(OH)2 showed a better organization of the collagen in relation to the adhesive systems Prime & Bond (p= .0159) and Adhese (p<0.0001). The affected dentin sealed with Prime & Bond promoted better increase of organized collagen areas in relation to Adhese (p = 0.0004). The infected dentin sealed with glass ionomer cement (p = 0.0018) or antibacterial cement (p = 0.0004) brought a significant increase in the organized collagen areas. Conclusions: Ca(OH)2 is indicated to seal affected dentin and glass ionomer cement and antibacterial cement may be used for treatment of infected dentin. The addition of antibiotics did not influence the restoration of the infected dentin.

Clinical, mineral and ultrastructural changes in carious dentin of primary molars after restoration

“…This intense blue colour would not be observed if only the cleaved collagen peptide fragments 1/4 or 3/4 were present in the carious dentin tissue. Similar results were obtained in affected and infected carious dentin after cavity sealing, regardless of the restorative material used.…”

Antimicrobial properties of conventional restorative filling materials and advances in antimicrobial properties of composite resins and glass ionomer cements—A literature review

“…Antibiotics have been added directly to restorative materials [27,28,[139][140][141][142]. Mixtures of ciprofloxacin, minocycline, cefaclor and metronidazole, have been added to GIC powder [27,139].…”

Bone Sialoprotein, Matrix Metalloproteinases and Type I Collagen Expression after Sealing Infected Caries Dentin in Primary Teeth

“…An increase in organized collagen area has been reported after sealing of infected dentin with glass ionomer cements [Pinheiro et al, 2010]. On the other hand, without cavity sealing, the degraded collagen level increased significantly in demineralized dentin specimens as compared to controls [Van Strijp et al, 2003], which again reinforces that cavity sealing alone was able to change the cavity environment and favor an odontoblastic response.…”