Repair of segmental long-bone defects by stem cell concentrate augmented scaffolds: a clinical and positron emission tomography - computed tomography analysis

Petri, Maximilian; Namazian, Ali; Wilke, Florian; Ettinger, Max; Stübig, Timo; Brand, Stephan; Bengel, Frank M.; Krettek, Christian; Berding, Georg; Jagodzinski, Michael

doi:10.1007/s00264-013-2087-y

Cited by 27 publications

(16 citation statements)

References 24 publications

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“…PB-MNCs seem as an interesting endothelial progenitor cell source for tissue engineering [7,8]. The PB-MNC-fraction is a mix of several cell types and the level of cells and cytokines differ between donors [20].…”

Section: Discussionmentioning

confidence: 99%

“…Endothelial progenitor cells are found among the CD34-positive fraction of peripheral blood (PB) mononuclear cells (MNC) [7], and have recently been shown important for angiogenesis and bone formation during clinical fracture healing [2]. Furthermore, clinical trials have demonstrated positive outcomes using PB or BM derived MNC-fractions in the repair of long-bone defects and non-unions [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced osteoblastic differentiation and bone formation in co-culture of human bone marrow mesenchymal stromal cells and peripheral blood mononuclear cells with exogenous VEGF

Joensuu

Uusitalo-Malmivaara

Alm

et al. 2015

Orthopaedics & Traumatology: Surgery & Research

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced osteoblastic differentiation and bone formation in co-culture of human bone marrow mesenchymal stromal cells and peripheral blood mononuclear cells with exogenous VEGF

Joensuu

Uusitalo-Malmivaara

Alm

et al. 2015

Orthopaedics & Traumatology: Surgery & Research

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“…1 Many different graft materials have been used for autologous bone grafts in clinical applications to treat bone defects, and demineralized bone matrix (DBM) has proven to be effective for bone grafting procedures. [2][3][4] Bone tissue engineering techniques that seed cells onto scaffolds provide an optimal method for repairing large bone defects. The utilization of such techniques has shown great potential, especially in disunion fractures where endochondral ossification occurs during wound healing.…”

Section: Introductionmentioning

confidence: 99%

Allogeneic adipose-derived stem cells regenerate bone in a critical-sized ulna segmental defect

Wen

Yan²,

et al. 2015

Exp Biol Med (Maywood)

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Adipose-derived stem cells (ASCs) with multilineage potential can be induced into osteoblasts, adipocytes and chondrocytes. ASCs as seed cell are widely used in the field of tissue engineering, but most studies either use autologous cells as the source or an immunodeficient animal as the host. In our present study, we explored the feasibility of applying allogeneic ASCs and demineralized bone matrix (DBM) scaffolds for repairing tubular bone defects without using immunosuppressive therapy. Allogeneic ASCs were expanded and seeded on DBM scaffolds and induced to differentiate along the osteogenic lineage. Eight Sprague-Dawley (SD) rats were used in this study and bilateral critical-sized defects (8 mm) of the ulna were created and divided into two groups: with ASC-DBM constructs or DBM alone. The systemic immune response and the extent of bone healing were evaluated post-operatively. Twenty-four weeks after implantation, digital radiography (DR) testing showed that new bones had formed in the experimental group. By contrast, no bone tissue formation was observed in the control group. This study demonstrated that allogeneic ASCs could promote bone regeneration and repair tubular bone defects combined with DBM by histologically typical bone without systemic immune response.

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“…Accumulating evidence has proved that vascularization is an important step toward the success of tissue regeneration (Wang et al, 2010). Petri and co-works claimed that stem cell concentrates can be an alternative to segmental bone regeneration for long-bone defects are larger than 3 cm (Petri et al, 2013). Critical size long bone defect could be completely re-grown with the aid of vascularization.…”

Section: Nspd Reduced the Expression Of Virulence-related Genesmentioning

confidence: 99%

An Evaluation of Norspermidine on Anti-fungal Effect on Mature Candida albicans Biofilms and Angiogenesis Potential of Dental Pulp Stem Cells

Cao

Xiang

et al. 2020

Front. Bioeng. Biotechnol.

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Norspermidine (Nspd) is a kind of polyamine molecule, which is common in eukaryotes and prokaryotes. It has been reported as a potential anti-biofilms agent of bacteria, but its anti-fungal effect remains unclear. Candida albicans (C. albicans) is a common opportunistic pathogen in oral cavity of human beings. C. albicans biofilm is often seen in dental caries. In this work, we aimed to study the effect of Nspd on mature Candida albicans biofilms and to investigate how Nspd would influence human dental pulp stem cells (DPSCs). Our biofilm assays indicated that 111.7 and 55.9 mM Nspd dispersed 48 h mature fungal biofilms and showed significant fungicidal effect. 27.9 and 14.0 mM Nspd showed moderate fungicidal effect. Live/dead staining echoed the fungicidal effect. 111.7-14.0 mM Nspd showed a dose-inhibitory effect on mature fungal biofilm, where 14.0 mM Nspd reduced the metabolic activity by half compared with blank control. Moreover, we demonstrated that 111.7-27.9 mM Nspd restrained the production of hyphae form of C. albicans via SEM. Low dose Nspd (27.9 and 14.0 mM) could significantly reduce virulence related gene expression in C. albicans biofilms. MTT assay displayed a dose effect relation between 2.5-0.08 mM Nspd and DPSCs viability, where 0.63 mM Nspd reduced the viable level of DPSCs to 75% compared with blank control. Live/dead staining of DPSCs did not show distinctive difference between 0.63 mM Nspd and blank control. Vascular differentiation assay showed capillary-like structure of inducted DPSCs culture with and without 0.63 mM Nspd suggesting that it did not significantly affect angiogenic differentiation of DPSCs. Nspd can penetrate remaining dentin at low level, which is confirmed by an in vitro caries model. In conclusion, our study indicated high dosage Nspd (111.7 and 55.9 mM) could effectively disrupt and kill mature fungal biofilms. Low dosage (27.9 and 14.0 mM) showed mild anti-fungal effect on mature C. albicans biofilms. Human DPSCs were tolerate to 0.08-0.63 mM Nspd, where viability was over 75%. 0.63 mM Nspd did not affect the proliferation and angiogenetic differentiation of DPSCs.

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Repair of segmental long-bone defects by stem cell concentrate augmented scaffolds: a clinical and positron emission tomography - computed tomography analysis

Cited by 27 publications

References 24 publications

Enhanced osteoblastic differentiation and bone formation in co-culture of human bone marrow mesenchymal stromal cells and peripheral blood mononuclear cells with exogenous VEGF

Enhanced osteoblastic differentiation and bone formation in co-culture of human bone marrow mesenchymal stromal cells and peripheral blood mononuclear cells with exogenous VEGF

Allogeneic adipose-derived stem cells regenerate bone in a critical-sized ulna segmental defect

An Evaluation of Norspermidine on Anti-fungal Effect on Mature Candida albicans Biofilms and Angiogenesis Potential of Dental Pulp Stem Cells

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