Repair of osteochondral defects with biphasic cartilage-calcium polyphosphate constructs in a Sheep model

Kandel, Rita A.; Grynpas, Marc D.; Pilliar, Robert M.; Lee, J; Wang, J; Waldman, Stephen D.; Zalzal, Paul; Hurtig, Mark

doi:10.1016/j.biomaterials.2006.03.005

Cited by 184 publications

(143 citation statements)

References 47 publications

(71 reference statements)

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“…This approach has also been utilized to generate cartilaginous tissue in vitro as well [121]. In vivo sheep studies have demonstrated that following implantation into focal defects in the joint, cartilage tissue thickness increases and its mechanical properties improve, confirming the utility of a scaffold-free tissue engineering approach [51]. Interestingly this approach can be used to form composite tissues, such as nucleus pulposus/cartilage (CEP) which is necessary for disc tissue engineering [36].…”

Section: What Is the Right Scaffold?mentioning

confidence: 99%

Tissue engineering and the intervertebral disc: the challenges

2008

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Disc degeneration is a common disorder. Although the back pain that can develop in association with this is rarely life-threatening, the annual cost in terms of morbidity, lost productivity, medical expenses and workers' compensation benefits is significant. Surgical intervention as practised currently is directed towards removing the damaged or altered tissue. Development of new treatment modalities is critical as there is a growing consensus that the strategies used currently for symptomatic degenerative disc disease may not be effective. Accordingly, there is a need to develop an entirely new way to treat this disorder; regenerative medicine and tissue engineering approaches appear particularly promising in this regard. This paper reviews some of the challenges that currently are limiting the clinical application of this approach to the treatment of disc degeneration.

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Section: What Is the Right Scaffold?mentioning

confidence: 99%

Tissue engineering and the intervertebral disc: the challenges

2008

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“…A number of studies performed in rabbit (Ball et al, 2004;Grigolo et al, 2001;Schaefer et al, 2002), sheep (Kandel et al, 2006), or goat (Niederauer et al, 2000) aimed at investigating the outcome of cartilage or osteochondral repair using engineered cartilage. Cells were seeded in the scaffolds either directly after isolation or after an expansion phase and further pre-cultured in vitro for 48 h (Niederauer et al, 2000), 7 d (Ball et al, 2004), 4 to 6 weeks (Schaefer et al, 2002), or 8 weeks (Kandel et al, 2006) before implantation in an orthotopic site. With only one exception (Niederauer et al, 2000), an improved healing was observed when cells were added to the scaffolds as compared to implantation of cell-free materials.…”

Section: Introductionmentioning

confidence: 99%

Influence of in vitro maturation of engineered cartilage on the outcome of osteochondral repair in a goat model

Miot

Brehm²,

Dickinson³

et al. 2012

eCM

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This study was designed to determine if the maturation stage of engineered cartilage implanted in a goat model of cartilage injury infl uences the repair outcome. Goat engineered cartilage was generated from autologous chondrocytes cultured in hyaluronic acid scaffolds using 2 d, 2 weeks or 6 weeks of pre-culture and implanted above hydroxyapatite/hyaluronic acid sponges into osteochondral defects. Control defects were left untreated or treated with cell-free scaffolds. The quality of repair tissues was assessed 8 weeks or 8 months post implantation by histological staining, modifi ed O'Driscoll scoring and biochemical analyses. Increasing pre-culture time resulted in progressive maturation of the grafts in vitro. After 8 weeks in vivo, the quality of the repair was not improved by any treatment. After 8 months, O'Driscoll histology scores indicated poor cartilage architecture for untreated (29.7 ± 1.6) and cell-free treated groups (24.3 ± 5.8). The histology score was improved when cellular grafts were implanted, with best scores observed for grafts pre-cultured for 2 weeks (16.3 ± 5.8). As compared to shorter pre-culture times, grafts cultured for 6 weeks (histology score: 22.3 ± 6.4) displayed highest type II/I collagen ratios but also inferior architecture of the surface and within the defect, as well as lower integration with native cartilage. Thus, pre-culture of engineered cartilage for 2 weeks achieved a suitable compromise between tissue maturity and structural/integrative properties of the repair tissue. The data demonstrate that the stage of development of engineered cartilage is an important parameter to be considered in designing cartilage repair strategies.

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“…The amounts previous study (10). Before testing, samples were placed of chondrocyte-like cells and cartilage-like extracellular in phosphate-buffered saline (PBS) at room temperature matrix in group III were more than those of group II as for 3-4 h to equilibrate.…”

Section: Introductionmentioning

confidence: 99%

Local Delivery of Autologous Platelet in Collagen Matrix Simulated in Situ Articular Cartilage Repair

Chen

Jiang

et al. 2009

Cell Transplant

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Bone marrow released by microfracture or full-thickness cartilage defect can initiate the in situ cartilage repair. However, it can only repair small cartilage defects (<2 cm 2 ). This study aimed to investigate whether autologous platelet-rich plasma (PRP) transplantation in collagen matrix can improve the in situ bone marrow-initiated cartilage repair. Full-thickness cartilage defects (diameter 4 mm, thickness 3 mm) in the patellar grooves of male New Zealand White rabbits were chosen as a model of in situ cartilage repair. They were treated with bilayer collagen scaffold (group II), PRP and bilayer collagen scaffold (group III), and untreated (group I), respectively (n = 11). The rabbits were sacrificed at 6 and 12 weeks after operation. The repaired tissues were processed for histology and for mechanical test. The results showed that at both 6 and 12 weeks, group III had the largest amounts of cartilage tissue, which restored a larger surface area of the cartilage defects. Moreover, group III had higher histological scores and more glycosaminoglycans (GAGs) content than those in the other two groups (p < 0.05). The Young's modulus of the repaired tissue in group II and group III was higher than that of group I (p < 0.05). Autologous PRP and bilayer collagen matrix stimulated the formation of cartilage tissues. The findings implicated that the combination of PRP with collagen matrix may repair larger cartilage defects that currently require complex autologous chondrocyte implantation (ACI) or osteochondral grafting.

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Repair of osteochondral defects with biphasic cartilage-calcium polyphosphate constructs in a Sheep model

Cited by 184 publications

References 47 publications

Tissue engineering and the intervertebral disc: the challenges

Tissue engineering and the intervertebral disc: the challenges

Influence of in vitro maturation of engineered cartilage on the outcome of osteochondral repair in a goat model

Local Delivery of Autologous Platelet in Collagen Matrix Simulated in Situ Articular Cartilage Repair

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