Repair of Osteochondral Defects With Grafts of Cultured Chondrocytes

Noguchi, Takashi; Oka, Masanori; Fujino, Madoka; Neo, Masashi; Yamamuro, Takao

doi:10.1097/00003086-199405000-00039

Cited by 78 publications

(47 citation statements)

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“…At the same time, they reported that histological monolayer cell infiltration was not observed and no immunological reaction by transplanted chondrocytes or collagen was seen (14). On the other hand, in their study, Noguchi et al (12), repaired osteochondral defects using culture chondrocyte grafts and compared allogenic and isogenic grafts. They reported that in early stages of defects (in the second and fourth weeks), which were treated using isogenic or allogenic chondrocytes, they were filled with hyaline cartilage, and in peripheral sections there was a light inflammatoric cell inflammation which was more clear in allogenic defects.…”

Section: Discussionmentioning

confidence: 97%

“…Nitekim, sağlıklı kedilerde Na geri emilimi %99'un üzerinde iken KBY olan kedilerde %87.2'den düşük olduğu bildirilmiştir (9,12). Kedilerde kan K seviyesinin 4.1 mEq/L (15.9 mg/dl) altına düştüğünde hipokalemi şekillendiği KBY olan kedilerde bu değerin 3.0 mEq/L'nin (11.7 mg/dl) altına düştüğü, KBY olan kedilerde K'un idrarla dışarı atılımı-nın artması (kaliüri) nedeniyle hipokalemi gözlendiği, böbrek tubuluslarından K geri emilimi normal kedilerde %89.9 iken KBY olan kedilerde %56.5 olduğu bildirilmiştir.…”

Section: Tartışma Ve Sonuçunclassified

“…Kedilerde kan K seviyesinin 4.1 mEq/L (15.9 mg/dl) altına düştüğünde hipokalemi şekillendiği KBY olan kedilerde bu değerin 3.0 mEq/L'nin (11.7 mg/dl) altına düştüğü, KBY olan kedilerde K'un idrarla dışarı atılımı-nın artması (kaliüri) nedeniyle hipokalemi gözlendiği, böbrek tubuluslarından K geri emilimi normal kedilerde %89.9 iken KBY olan kedilerde %56.5 olduğu bildirilmiştir. İdrarla aşırı K atılımı idrar miktarında artış, Na geri emilimde azalma ile birlikte görülebilir (9,12).…”

Section: Tartışma Ve Sonuçunclassified

“…Therefore, developed therapy methods are needed in cartilaginous tissue damages (1,4). Insufficiency (loss of function) and pain induced by joint cartilage damages led researchers to find ways to facilitate and increase cartilage repair (12,13,17,18,20). In order to have repaired or regenerated cartilage to function as joint tissue sufficiently, normal painless motion synovial joint should be restored.…”

Section: Introductionmentioning

confidence: 99%

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Kronik böbrek yetmezliği belirtileri gösteren kedilerde biyokimyasal ve hematolojik değişiklikler

Altintaş¹,

Üren²,

Pekcan³

et al. 2006

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Araştırmada, kronik böbrek yetmezliği (KBY) gözlenen kedilerde hematolojik ve biyokimyasal değişikliklerin incelenmesi ve bulguların hastalığın derecelendirilmesinde kullanılması amaçlanmıştır. KBY tespit edilen ve yaşları 3-12 arasında değişen 22 dişi kedi ile aynı yaşlarda ve klinik olarak sağlıklı görünen 10 dişi kedi (kontrol) araştırmanın hayvan materyalini oluşturmuştur. Hayvanlara ait idrar örneklerinde protein varlığı ve dansite; kan örneklerinde hemoglobin, hematokrit değer, alyuvar ve akyuvar sayıları ile akyuvarların % dağılımları; serum örneklerinde ise toplam protein, üre, kreatinin, sodyum, potasyum, kalsiyum, fosfor, osteokalsin (OC), toplam ALP (t-ALP) ve kemik spesifik ALP (k-ALP) değerleri ölçülmüştür. KBY'li kedilerde poliüri, idrar dansitesinde düşüş, azotemi, hipokalemi, hiponatremi, hiperfosfatemi, hiperproteinemi, normositik normokromik anemi ve akyuvar artışı saptanmış; serum osteokalsin değerleri hasta ve kontrol grupta ölçüm sınırlarının altında (<1ng/ml) bulunmuştur. Kan serumunda üre ve kreatinin değerlerinin KBY'li kedilerde "üremi" düzeylerine (320.7±38.5 mg/dl) yükseldiği (p<0.001); kreatinin değer-lerinin de (6.77±0.75 mg/dl) hastalığın ciddi boyutlarda olduğunu gösterdiği saptanmıştır. Serum Na ve K değerlerinin hasta kedilerde önemli derecede düştüğü (p<0.05); Ca değerlerinde istatistik önemli bir değişiklik gözlenmezken (p>0.05) Pi değerlerinin önemli derecede (p<0.001) artış gösterdiği (hiperfosfatemi) saptanmış ve değişiklikler glomeruler filtrasyon hızında düşüş ve tubuler geri emilimde bozukluk ile ilişkilendirilmiştir. İncelenen parametreler kümeleme testi ile değerlendirilerek kedilerde KBY (1+)-(3+) arasında derecelendirilmiş ve 4 hastada şiddetli, 9 hastada orta ve geri kalan 9 hastada ise hafif seyrettiği saptanmıştır. Kan hematokrit yüzdesi ve alyuvar sayısındaki önemli düşüşler (p<0.01) KBY ile uyumlu bulunmuştur (normositik normokromik anemi) ve akyuvar sayısındaki artış olası bir sekonder enfeksiyona bağlanmıştır. Kan hemoglobin değerlerindeki düşüş belirgin olmasına rağmen istatistik önemli bulunmamıştır (p>0.05). Serum t-ALP ve k-ALP düzeylerindeki önemli düşüşler; Ca/P dengesindeki bozukluk ve düşük osteokalsin düzeyleri kemik metabolizmasının kronik yetmezlikten henüz belirgin şekilde etkilenmediği yönünde değer-lendirilmiştir.Anahtar sözcükler: Biyokimyasal belirtiler, hematolojik belirtiler, kedi, kronik böbrek yetmezliği. Biochemical and hematological changes in cats with chronic renal failureSummary: In this study, it is aimed to evaluate the bone metabolism, biochemical and haematological changes of cats with chronic renal failure. 22 female cats aging between 3-12 years with Chronic Renal Failure (CRF) and for control group 10 clinicaly healty female cats aging nearly the same with the patients constituted the research's animal material. Urine samples densities were determined and assayed for the presence of protein. Blood count and total protein, urea, creatinine, sodium, potassium, calcium, phosphorus, osteocalcine, total ALP and bone ALP assays h...

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Section: Discussionmentioning

confidence: 97%

Section: Tartışma Ve Sonuçunclassified

Section: Introductionmentioning

confidence: 99%

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Kronik böbrek yetmezliği belirtileri gösteren kedilerde biyokimyasal ve hematolojik değişiklikler

Altintaş¹,

Üren²,

Pekcan³

et al. 2006

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

show abstract

“…Kawabe and Yoshinao [15] identified an immune rejection response in rabbits, which was associated with premature degeneration of the newly formed healing tissue. Noguchi et al [20] found no difference in the healing of osteochondral defects in rats that had been treated with isogenic chondrocytes compared with those that had been treated with allogenic chondrocytes, both of which were carried in collagen gels.…”

Section: Discussionmentioning

confidence: 99%

Rabbit articular cartilage defects treated by allogenic chondrocyte transplantation

Boopalan

Sathishkumar

Kumar

et al. 2006

International Orthopaedics (SICOT)

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Articular cartilage defects have a poor capacity for repair. Most of the current treatment options result in the formation of fibro-cartilage, which is functionally inferior to normal hyaline articular cartilage. We studied the effectiveness of allogenic chondrocyte transplantation for focal articular cartilage defects in rabbits. Chondrocytes were cultured in vitro from cartilage harvested from the knee joints of a New Zealand White rabbit. A 3 mm defect was created in the articular cartilage of both knees in other rabbits. The cultured allogenic chondrocytes were transplanted into the defect in the right knees and closed with a periosteal flap, while the defects in the left knees served as controls and were closed with a periosteal flap alone, without chondrocytes. Healing of the defects was assessed at 12 weeks by histological studies. Allogenic chondrocyte transplantation significantly increased the amount of newly formed repair tissue (P=0.04) compared with that found in the control knees. The histological quality score of the repair tissue was significantly better (P=0.05), with more hyaline characteristics in the knees treated with allogenic chondrocytes than in the control knees. Articular cartilage defects treated with allogenic chondrocyte transplantation result in better repair tissue formation with hyaline characteristics than those in control knees.Résumé Nous avons étudié l'efficacité de la transplantation de chondrocytes allogènes pour des pertes de substances localisées chez le lapin. Une perte de substance de 3 mm a été crée au niveau des 2 genoux. Des chondrocytes ont été cultivés in vitro à partir de prélèvements fait sur les genoux de lapin blanc de Nouvelle Zélande. Les chondrocytes cultivés ont été transplantés dans le defect du genou droit et enfermés sous un lambeau périosté tandis que le genou gauche servait de contrôle avec un lambeau périosté sans chondrocyte. La cicatrisation cartilagineuse a été étudié histologiquement à 12 semaines. La transplantation de chondrocytes allogènes augmente significativement la quantité de tissu de réparation en comparant aux genoux de contrôle (p=0.04). Le score histologique est meilleur que dans le groupe de contrôle.

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Retention of hyaluronic acid in alginate beads: Aspects forin vitro cartilage engineering

Lindenhayn

Perka

Spitzer

et al. 1999

J. Biomed. Mater. Res.

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Alginate has been used successfully for three-dimensional chondrocyte cultures and may be important for cartilage transplant formation. However, alginate is not a natural component of the cartilage matrix. The aim of this study was (a) to supplement alginate with the extracellular matrix component hyaluronic acid; and (b) to analyze the hyaluronic acid retention in different alginate gels. Hyaluronan is assumed to improve proteoglycan retention and may be important for in vitro matrix formation, tissue turgor, and biomechanical quality. Alginate and hyaluronan were mixed with chondrocytes and polymerized as were alginate, hyaluronan, and fibrinogen. [3H]hyaluronan was used to quantitate the leakage of hyaluronan from the gel beads. After 28 days in culture, 1.2% alginate beads supplemented with 0.26% hyaluronan contained only 9% of the initial amount of hyaluronan whereas 2.4% alginate beads still contained about 55% of the initial 0.22% hyaluronan. Release of hyaluronan from the beads was significantly lower if the beads additionally contained fibrin. Alginate beads supplemented with hyaluronan or fibrin showed increased chondrocyte proliferation compared to controls. Supplemented hyaluronan greatly diffuses out of alginate gels of lower densities. It must be assumed also that most of the hyaluronan newly synthesized by chondrocytes in these cells diffuses into the surrounding culture medium. The in vitro development of a sufficiently hygroscopic cartilage ground substance therefore may be very limited. Sufficient hyaluronic acid retention can be achieved in alginate gels with concentrations above 1.2% or by addition of fibrin.

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Repair of Osteochondral Defects With Grafts of Cultured Chondrocytes

Cited by 78 publications

References 0 publications

Kronik böbrek yetmezliği belirtileri gösteren kedilerde biyokimyasal ve hematolojik değişiklikler

Kronik böbrek yetmezliği belirtileri gösteren kedilerde biyokimyasal ve hematolojik değişiklikler

Rabbit articular cartilage defects treated by allogenic chondrocyte transplantation

Retention of hyaluronic acid in alginate beads: Aspects forin vitro cartilage engineering

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