Repair of nerve defect with chitosan graft supplemented by uncultured characterized stromal vascular fraction in streptozotocin induced diabetic rats

Abstract

Regenerative properties of stem cells at the service of nerve repair have been initiated during recent decades. Effects of transplantation of characterized uncultured stromal vascular fraction (SVF) on peripheral nerve regeneration were studied using a rat sciatic nerve transection model. A 10-mm sciatic nerve defect was bridged using a chitosan conduit filled with SVF. In control group, chitosan conduit was filled with phosphate-buffered saline alone. The regenerated nerve fibers were studied 4 weeks, 8 weeks, and 12 weeks after surgery. In sham-operated group, the sciatic nerve was only exposed and manipulated. Behavioral and Functional studies confirmed faster recovery of regenerated axons in SVF transplanted animals than in control group (P < 0.05). Gastrocnemius muscle mass in SVF transplanted animals was found to be significantly more than that in control group. Morphometric indices of the regenerated fibers showed the number and diameter of the myelinated fibers were significantly higher in SVF transplanted animals than in control group. In immunohistochemistry, location of reactions to S-100 in SVF transplanted animals was clearly more positive than that in control group. SVF transplantation combined with chitosan conduit could be considered as a readily accessible source of stromal cells that improve functional recovery of sciatic nerve.

Advancing biomaterials of human origin for tissue engineering

“…Based on this concept, non-expanded SVF cells (freshly procured without cell culture) were used to form new adipose tissue in vitro using a porous collagen/gelatin sponge as a scaffold [575]. Recent evidence also suggests that SVF transplantation combined with a chitosan conduit may be considered as a readily available stromal cell source for ameliorating the functional recovery of the sciatic nerve [576]. Furthermore, an intratendinous injection of uncultured adipose-derived SVF cells results in improved structural and mechanical properties in tendon repairs and could be an effective modality for the treatment of tendon injury [577].…”

Facial‐nerve regeneration ability of a hybrid artificial nerve conduit containing uncultured adipose‐derived stromal vascular fraction: An experimental study

“…Furthermore, because no special equipment is necessary for preparation, SVF can be a cell source in a variety of clinical settings. However, the proportion of ASC in SVF is inconsistent, and because of this, to date, only a very small number of basic studies have reported the application of uncultured‐SVF in peripheral nerve regeneration . The authors speculated that the use of optimal concentration of uncultured‐SVF might improve the potential of hybrid nerve conduits in facial nerve regeneration.…”

“…However, the proportion of ASC in SVF is inconsistent, and because of this, to date, only a very small number of basic studies have reported the application of uncultured-SVF in peripheral nerve regeneration. [16][17][18] The authors speculated that the use of optimal concentration of uncultured-SVF might improve the potential of hybrid nerve conduits in facial nerve regeneration. This study used hybrid artificial nerve conduits with uncultured-SVF for treating a 7-mm defect made in the buccal branches of the facial nerve in a rat transplantation model, and histological and physiological analyses were employed to examine 1) the degree of SVF efficacy in facial nerve regeneration and 2) the concentration of SVF to be infused into an artificial nerve conduit to achieve optimal outcomes.…”

The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune‐inhibitory inflammatory cells

“…In conclusion, the entire u-ADSC population can be used to treat damaged tissue [18,[30][31][32][33][34][35][36]; they contain a substantial number of MSCs and they enable the repair and restoration of inflammation-mediated liver damage. These cells include an immune-suppressive M -phenotype subfraction, which contributes to amelioration of inflammation-mediated tissue damage.…”