Repair of meniscal lesions using a scaffold-free tissue-engineered construct derived from allogenic synovial MSCs in a miniature swine model

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering

“…Menisci before and after decellularization were fixed in 10% (v/v) neutral buffered formalin (Sigma-Aldrich) overnight, dehydrated through an ethanol series, and embedded in paraffin. Specimen longitudinal sections of 5-10 mm in thickness were stained with hematoxylin and eosin (H&E) (Sigma-Aldrich) to visualize nuclei, or stained with Alcian blue (SigmaAldrich) for GAG deposition, 34,35 or stained with Masson's trichrome (Sigma-Aldrich) to observe total collagen. 36,37 Type II collagen was immunolocalized by an anti-type II collagen antibody (bs-0709r; Bioss) with a 1:100 dilution in Tris-buffered saline with Tween 20 solution, and the signal was amplified with an UltraVision Quanto Detection System (Thermo Scientific) for 10 min, followed by diaminobenzidine for visualization as described by the manufacturer.…”

In Vitro Repair of Meniscal Radial Tear Using Aligned Electrospun Nanofibrous Scaffold

“…While MSCs have been employed in many of the cellbased meniscal repair studies, 37,38 meniscus-derived cells could be a good cell source because these cells showed strong proliferative and fibrocartilaginous differentiation capacities, as reported in previous studies. 29,30 Also, regarding the method of cell isolation, we isolated MFCs from small pieces of meniscal tissue without collagenase, using a previously published method.…”

Biological Augmentation and Tissue Engineering Approaches in Meniscus Surgery

“…They reported a significant healing rate when MSCs were used. Moriguchi et al 37 developed an experimental tissue-engineered construct of synovial MSCs and ascorbic acid, showing significantly improved healing of 4-mm defects in the avascular zone of the meniscus after 6 months. Finally, Esparza et al 38 assessed in vivo collagen gene expression by synovial meniscus cells after stimulation to vascular endothelial growth factor (VEGF), TGF-b, bFGF, and IGF.…”