Repair of infarcted myocardium using mesenchymal stem cell seeded small intestinal submucosa in rabbits

Tan, Mei Yun; Zhi, Wei; Ren, Wei; Huang, Yong; Zhou, Kun; Tan, Bo; Deng, Li; Luo, Jing; Li, Xiu Qun; Xie, Hui; Yang, Zhe

doi:10.1016/j.biomaterials.2009.02.013

Cited by 106 publications

(65 citation statements)

References 34 publications

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“…11 To note, CL-MSC within 3D grafts displayed acceptable early survival in vivo either with immediate vascular supply or not. Although several studies in animal models of MI have demonstrated the ability of transplanted BM-MSC to engraft and differentiate into cardiomyocytes and vasculature cells, [25][26][27] we were not able to detect the expression of mature cardiomyocyte or vascular cell markers in engrafted CL-MSC despite their ability to express genes important in cardiac differentiation, such as Gata4 and NKX2.5 by CL-MSC before an epicardial graft implantation. Cardiomyogenic differentiation of BM-MSC has been reported in both allogeneic 22,28 and xenograft models.…”

mentioning

confidence: 38%

Cord Lining-Mesenchymal Stem Cells Graft Supplemented with an Omental Flap Induces Myocardial Revascularization and Ameliorates Cardiac Dysfunction in a Rat Model of Chronic Ischemic Heart Failure

Lilyanna

Martinez

et al. 2013

Tissue Engineering Part A

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Myocardial restoration using tissue-engineered grafts to regenerate the ischemic myocardium offers improved donor cell retention, yet a limited cell survival resulting from poor vascularization needs to be addressed. A cell type derived from the subamnion, namely, cord-lining mesenchymal stem cells (CL-MSC), has recently been identified. Here we present a restorative strategy that combines a fibrin graft containing human CL-MSC and omental flap providing, thereby, cell-, structural-, and angiogenic support to the injured myocardium. The graft consisted of a mixture of 2 · 10 6 CL-MSC-GFP-Fluc and fibrin. Myocardial infarction (MI) was induced in nude rats and following confirmation of ensued heart failure with echocardiography 2 weeks after injury, therapeutic intervention was performed as follows: untreated (MI, n = 7), CL-MSC graft (CL-MSCG, n = 8), CL-MSCG and omental flap (CL-MSCG + OM, n = 11), and omental flap (OM, n = 8). In vivo bioluminescence imaging at 1, 3, 7, and 14 days post-treatment indicated comparable early donor cell viability between the CL-MSCG and CL-MSCG + OM. Treatment with CL-MSCG + OM improved the myocardial function as assessed by the measurement of end-diastolic left ventricular (LV) pressure (3.53 -0.34 vs. 5.21 -0.54 mmHg, p < 0.05), contractility ( + dP/dt, 3383.8 -250.78 mmHg vs. 2464.9 -191.8 mmHg, p < 0.05), and the relaxation rate (-dP/ dt, -2707.2 -250.7 mmHg vs. 1948.7 -207.8 mmHg, p < 0.05), compared to MI control 6 weeks after ischemic injury. Furthermore, evidence of a 20.32% increase in the ejection fraction was observed in CL-MSCG + OM rats from week 2 to 6 after injury. Both CL-MSCG and CL-MSCG + OM led to an enhanced cardiac output (p < 0.05), and attenuated the infarct size (35.7% -4.2% and 34.7% -4.8%), as compared to MI (60.7% -3.1%; p < 0.01 and p < 0.001, respectively). All treated groups had a higher arteriole density than controls. Yet, a higher amount of functional blood vessels, and a 20-fold increase in arteriole numbers were found in CL-MSCG + OM. Altogether, CL-MSCGs supplemented with vascular supply have the potential to repair the failing, chronically ischemic heart by improving myocardial revascularization, attenuating remodeling, and ameliorating cardiac dysfunction.

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mentioning

confidence: 38%

Cord Lining-Mesenchymal Stem Cells Graft Supplemented with an Omental Flap Induces Myocardial Revascularization and Ameliorates Cardiac Dysfunction in a Rat Model of Chronic Ischemic Heart Failure

Lilyanna

Martinez

et al. 2013

Tissue Engineering Part A

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“…Although the transplantations of both SIS and MSC-seeded SIS into the heart of an infarcted rabbit model induced significant improvement of the heart function, the MSC-seeded SIS was more effective [58]. The migration of MSCs from SIS into the infarcted area and the differentiation of MSCs into cardiomyocytes and smooth muscle cells were shown [58]. Chachques et al have performed clinical trials by using autologous bone marrow cell-seeded 3D collagen type I matrix and showed the feasibility and safety of the tissue transplantation [59].…”

Section: Three-dimensional Tissues Fabricated By Using Autologous Celmentioning

confidence: 90%

“…The transplantation of bone marrow cell-seeded PGCL scaffold effectively attenuated LV remodeling and LV systolic dysfunction in a rat infarction model via the induction of neovascularization and the differentiation of stem cells into cardiomyocytes [57]. Tan et al used small intestinal submucosa (SIS) as 3D scaffold [58]. Although the transplantations of both SIS and MSC-seeded SIS into the heart of an infarcted rabbit model induced significant improvement of the heart function, the MSC-seeded SIS was more effective [58].…”

Section: Three-dimensional Tissues Fabricated By Using Autologous Celmentioning

confidence: 99%

“…Tan et al used small intestinal submucosa (SIS) as 3D scaffold [58]. Although the transplantations of both SIS and MSC-seeded SIS into the heart of an infarcted rabbit model induced significant improvement of the heart function, the MSC-seeded SIS was more effective [58]. The migration of MSCs from SIS into the infarcted area and the differentiation of MSCs into cardiomyocytes and smooth muscle cells were shown [58].…”

Section: Three-dimensional Tissues Fabricated By Using Autologous Celmentioning

confidence: 99%

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Concise Review: Cell Therapy and Tissue Engineering for Cardiovascular Disease

Haraguchi

Shimizu

Yamato

et al. 2012

Stem Cells Translational Medicine

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“…Badylak et al (1989) first made a vascular graft in dogs using SIS successfully. SIS has been also used in different organ systems (Gastel et al, 2001) and it has given good results on host tissue regeneration (Ashley et al, 2010;Hoeppner et al, 2009) and functional recovery (Tan et al, 2009). The porous nature and three-dimensional (3D) micro-architecture of SIS enable cells to adhere, grow and migrate (Badylak et al, 1999;Ferrand et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Quantification of MSCs involved in wound healing: use of SIS to transfer MSCs to wound site and quantification of MSCs involved in skin wound healing

Yeum

Park

Lee

et al. 2012

J Tissue Eng Regen Med

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Mesenchymal stem cells (MSCs) are known to be effective in wound healing, but not much has been reported on quantitative correlations between MSCs injected into the wound site and MSCs that actually participate in wound healing. This study traced MSCs participating in wound healing by using small intestinal submucosa (SIS) as a cell carrier, identified their moving path and calculated the number of MSCs involved in wound healing. First, MSCs were isolated from the nude mouse and 1 Â 10 6 cells were seeded onto the centre of the SIS. MSC-seeded SIS complexes were injected onto full-thickness skin wounds made on the dorsum of nude mice. Tracing of MSC-seeded SIS complex transplanted to the wound site revealed that 27.6% of the MSCs were migrated to the wound site at the first attempt. Second, repeated injection of additional MSCs did not increase the number of MSCs participating in wound healing beyond a certain constant maximum amount. The number of MSCs present in the wound site remains constant in the range 2-3 Â 10 5 from day 1 to day 10. The expression of skin regenerationrelated growth factors was confirmed by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). MSCs participating in wound healing were found not only to suppress inflammation of the wound but also to increase the skin regeneration-related growth factors that enable the recovery of the skin. An optimal number of about 3 Â 10 5 MSCs injected into the site was found to adapt themselves to the skin wound-healing process effectively.

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Repair of infarcted myocardium using mesenchymal stem cell seeded small intestinal submucosa in rabbits

Cited by 106 publications

References 34 publications

Cord Lining-Mesenchymal Stem Cells Graft Supplemented with an Omental Flap Induces Myocardial Revascularization and Ameliorates Cardiac Dysfunction in a Rat Model of Chronic Ischemic Heart Failure

Cord Lining-Mesenchymal Stem Cells Graft Supplemented with an Omental Flap Induces Myocardial Revascularization and Ameliorates Cardiac Dysfunction in a Rat Model of Chronic Ischemic Heart Failure

Concise Review: Cell Therapy and Tissue Engineering for Cardiovascular Disease

Quantification of MSCs involved in wound healing: use of SIS to transfer MSCs to wound site and quantification of MSCs involved in skin wound healing

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