Repair of Infarcted Myocardium Mediated by Transplanted Bone Marrow–Derived CD34
            <sup>+</sup>
            Stem Cells in a Nonhuman Primate Model

Yoshioka, Toru; Ageyama, Naohide; Shibata, Hiroaki; Yasu, Takanori; Misawa, Yoshio; Takeuchi, Koichi; Matsui, Keiji; Yamamoto, Keiji; Terao, Keiji; Shimada, Kazuyuki; Ikeda, Uichi; Ozawa, Keiya; Hanazono, Yutaka

doi:10.1634/stemcells.2004-0200

Cited by 116 publications

(71 citation statements)

References 36 publications

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“…[33][34][35][36][37] We elected to use SIVderived lentiviral vectors for this study based on reports that they are more efficient for transduction of simian stem cells. 19,20,[38][39][40] Our results using SIV-based aGT constructs are consistent with those findings. Under nonmyeloablative conditions, however, we were not able to achieve the levels of engraftment reported by Hanawa et al 19 using an SIV-based EGFP-expressing vector (marking up to 14% of CD3 + and 17% CD20 + cells 7 months after transfer).…”

Section: Discussionsupporting

confidence: 91%

Gene therapy to inhibit xenoantibody production using lentiviral vectors in non-human primates

et al. 2006

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Section: Discussionsupporting

confidence: 91%

Gene therapy to inhibit xenoantibody production using lentiviral vectors in non-human primates

et al. 2006

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“…The idea that native stem cells can be successfully manipulated has gained support from recent studies showing that tissue repair by implanted stem cells is mediated by an indirect, or "bystander," effect on resident cells, rather than a direct tissue integration of the introduced stem cells (56)(57)(58)(59)(60). Mechanistically, these bystander effects are not clearly understood but could be due to the support provided by growth factors that would then trigger/enhance the intrinsic repair response or nuclear fusion (59).…”

Section: In Situ Tissue Engineeringmentioning

confidence: 99%

“…Mechanistically, these bystander effects are not clearly understood but could be due to the support provided by growth factors that would then trigger/enhance the intrinsic repair response or nuclear fusion (59). However, in the case of MSCs, it remains to be determined whether these bystander effects are specific to MSCs or whether they represent an intrinsic property of stromal cells in general (60).…”

Section: In Situ Tissue Engineeringmentioning

confidence: 99%

Lessons from musculoskeletal stem cell research: The key to successful regenerative medicine development

McGonagle

Bari

Arnold

et al. 2007

Arthritis & Rheumatism

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Polystyrene and poly(butyl acrylate) were grafted from silicon wafer surface by reversible addition‐fragmentation chain transfer (RAFT) polymerization. Three RAFT agents were immobilized onto silicon wafer through their leaving/initiating groups (R group). Grafting polymerization of butyl acrylate (BA) and styrene (St) was then carried out from the immobilized RAFT agents. The immobilization of the RAFT agents and the subsequent grafting polymerization of St and BA were evaluated by ellipsometry and X‐ray photoelectron spectroscopy. It was found that type of monomer, structure of RAFT agent, and local RAFT concentration on the surface have dramatic influences on the thickness of grafted polymer layer. The grafting polymerization with more severe rate retardation effect yielded thinner polymer films on the silicon wafer. Selection of a RAFT agent with little rate retardation was critical in the grafting polymerization to achieve thick films. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 970–978, 2008

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“…This finding may be due to the lower reactivity of host vascular endothelial cells to growth factors in severe ischemic regions. The average blood flow ratio in periinfarction to nonischemic regions of experimental myocardial infarction models or stroke patients is w25% (Odano et al 1993, Yoshioka et al 2005. Thus, we need to clarify whether oxidative stress induced by a high-glucose concentration under moderate, rather than severe hypoxia (5% O 2 ; 25% of normal oxygen concentration), affects the production of angiogenic growth factors through HIF-1a induction in MSCs.…”

Section: Introductionmentioning

confidence: 99%

Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells

Ishizuka¹,

Hinata²,

Watanabe³

2010

Journal of Endocrinology

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Previous reports have shown that the paracrine system may be an important mediator in bone-marrow-derived mesenchymal stem cell (MSC) therapy for ischemic diseases. Hyperglycemia and hypoxia have been associated with increased levels of reactive oxygen species; oxidative stress may therefore influence the paracrine effects of MSCs under hypoxic conditions in diabetic patients, although the mechanism underlying this effect remains unknown. Hypoxia-inducible factor 1a (HIF-1a) regulates the transcription of hypoxia-inducible genes. We determined the effect of high-glucose concentrations on the production of angiogenic growth factors via HIF-1a induction in hypoxic MSCs. MSCs were cultured with different glucose concentration (5 . 6, 11, 20, or 30 mM) for 24 h. The cells were then incubated in a hypoxic chamber (5% O 2 ) or under normoxia (21% O 2 ) for 6 or 24 h. Protein levels of HIF-1a, vascular endothelial growth factor A 165 (VEGF-A 165 ), and platelet-derived growth factor B (PDGF-B) were attenuated by glucose in hypoxic MSCs in a dose-dependent manner. Treatment with MG132, a specific inhibitor of proteasome activity, significantly reversed the inhibitory effect of high-glucose concentrations in hypoxic MSCs. 4-Hydroxyl-tetramethylpiperidin-oxyl (a cell-permeable superoxide scavenger) or Apocynin (a NADPH oxidase inhibitor) significantly reversed glucose-induced attenuation of VEGF-A 165 , PDGF-B, and HIF-1a protein levels. Stimulation with a high-glucose concentration (30 mM) significantly increased intracellular superoxide levels in hypoxic MSCs. Our results suggest that in hypoxic MSCs the increase in intracellular superoxide levels induced by high-glucose concentrations may attenuate hypoxia-induced HIF-1a expression, which in turn attenuates hypoxiainduced VEGF-A 165 and PDGF-B transcription.

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Repair of Infarcted Myocardium Mediated by Transplanted Bone Marrow–Derived CD34 ⁺ Stem Cells in a Nonhuman Primate Model

Abstract: Stem Cells 2005;23:355-364

Cited by 116 publications

References 36 publications

Gene therapy to inhibit xenoantibody production using lentiviral vectors in non-human primates

Gene therapy to inhibit xenoantibody production using lentiviral vectors in non-human primates

Lessons from musculoskeletal stem cell research: The key to successful regenerative medicine development

Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells

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Repair of Infarcted Myocardium Mediated by Transplanted Bone Marrow–Derived CD34 + Stem Cells in a Nonhuman Primate Model

Abstract: Stem Cells 2005;23:355-364

Cited by 116 publications

References 36 publications

Gene therapy to inhibit xenoantibody production using lentiviral vectors in non-human primates

Gene therapy to inhibit xenoantibody production using lentiviral vectors in non-human primates

Lessons from musculoskeletal stem cell research: The key to successful regenerative medicine development

Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells

Contact Info

Product

Resources

About

Repair of Infarcted Myocardium Mediated by Transplanted Bone Marrow–Derived CD34 ⁺ Stem Cells in a Nonhuman Primate Model