Repair of Experimental Alveolar Bone Defects by Tissue-Engineered Bone

Weng, Yang; Wang, Min; Liu, Wei; Hu, Xiaojie; Chai, Gang; Yan, Qiaomei; Zhu, Lan; Cui, Lei; Cao, Yang

doi:10.1089/ten.2006.12.ft-142

Cited by 16 publications

(29 citation statements)

References 24 publications

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“…Although autologous bone grafts and the free flap technique still remain the standard in reconstruction of mandibular lesions, tissue engineering is a promising option to existing surgical procedures. 11 In contrast to a large autogenous bone graft, tissue engineering requires only a small amount of alveolar cells to create new functional tissue. The current study demonstrates that the critical-sized defects of the rat mandible can be repaired by albumin serum-derived scaffold seeded with human-cultured osteoblasts obtained from a small cancellous bone sample.…”

Section: Discussionmentioning

confidence: 99%

Repair of Rat Mandibular Bone Defects by Alveolar Osteoblasts in a Novel Plasma-Derived Albumin Scaffold

Gallego

Junquera²,

García³

et al. 2010

Tissue Engineering Part A

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Repair of bone deficiencies in the craniofacial skeleton remains a difficult clinical problem. The aim of this study was to evaluate a novel albumin scaffold seeded with human alveolar osteoblasts and implanted into experimental mandibular defects. An experimental solid protein scaffold was prepared with human plasmatic albumin crossed with a glutaraldehyde-type agent. Microstructure of scaffold and mechanical properties were examined using scanning electron microscopy and a stress-controlled rheometer. Bilateral critical mandibular defects were created in eight immunodeficient rats. Defects of the right side of the mandibles received the cell-scaffold construct in all animals. All left mandibular defects were left untreated as blank controls. Sections of the defects were collected at 5, 8, and 11 weeks postsurgery and processed for histological and immunohistochemical observation, computed tomography examination, and computed tomography digital analysis. Histologically, bone formation was observed in both groups at 5 weeks postsurgery, and the engineered bone became more mature after 8 and 11 weeks, which was similar to normal bone. The origin of bone-forming cells within the defects and the localization of implanted human osteoblasts were confirmed by human vimentin expression. No bone formation could be observed at any control defect. Bone density after 8 weeks was significantly higher than that of the 5-week group (p = 0.02), and significant differences were also observed between 8 and 11 weeks (p< 0.01). The results indicate the clinical feasibility of albumin scaffold loaded with human alveolar cells and that it can be used as a good alternative for bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Repair of Rat Mandibular Bone Defects by Alveolar Osteoblasts in a Novel Plasma-Derived Albumin Scaffold

Gallego

Junquera²,

García³

et al. 2010

Tissue Engineering Part A

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“…4 Nevertheless, the concept that the employment of MSCs promotes the global regeneration outcome is widely accepted, such that for example, MSCs are already engaged by default in regenerating the damaged periodontium. [5][6][7][8] These findings are not surprising because MSCs are attractive candidates for hard and soft tissue regeneration due to their inherent mesenchymal nature. Nevertheless, little is known about the precise events of MSC action in this complex multi-tissue environment resulting in new functional tooth attachment.…”

Section: Introductionmentioning

confidence: 98%

Crosstalk on Cell Behavior in Interactive Cocultures of hMSCs with Various Oral Cell Types

Proksch

Steinberg

Stampf

et al. 2012

Tissue Engineering Part A

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When prospectively applied for regenerative therapies, human bone-marrow-derived mesenchymal stem cells (hMSCs) interact with the locally residing host cells. With respect to the developmentally particular origin of oral cells, little is known about the putatively discriminative behavioral responses of hMSCs in interaction with various oral cell types, including human alveolar bone osteoblasts (hOAs), periodontal ligament fibroblasts (hPDLs), and gingival fibroblasts (hGFs). To assess the crosstalk between hMSCs and oral cells, interactive cocultures were established by combining well-characterized hMSCs with hOAs, hPDLs, or hGFs, and the behavioral hMSC aspects, that is, proliferation and gene expression, were measured by employing a 5-bromo-2'-deoxyuridine assay and real-time polymerase chain reaction, while apoptosis was quantified by in situ cell death detection kit. hMSCs expressed the typical antigen spectrum lacking CD34, CD45, CD14, CD19, and HLA-DR, while expressing CD73, CD90, and CD105, and could successfully be transformed into adipocytes, osteocytes, and chondrocytes. Monocultured control hMSCs proliferated readily, whereas a general reduction of BrdU-labeled cells was observed in cocultures. Globally, upon extending time periods, interactive coculture combinations of hMSCs with hOAs reduced both osteogenic gene and stem cell marker transcription in hMSCs, a phenomenon appearing less pronounced by combining hMSCs with hPDLs, such that the observed effects in terms of proliferation and gene expression followed the same ranking: hOAs>hGFs>hPDLs. Vice versa, in interactive hMSC cocultures, the cell survival rate was significantly increased, irrespective from the combined coculture cell counterpart. Our results show for the first time that behavior of hMSCs reflected by proliferation and gene expression was governed by interaction with various oral cells in a cell-type-discriminative manner. In addition, hMSC coculture restrains apoptosis, such that influences on cell behavior appear as a crosstalk. In summary, interactive cocultures render the basis for a prospective prediction of mutual cell behavior in hMSC-based oral tissue regeneration disclosing that oral cells shift hMSC behavior from proliferation to differentiation and apoptosis-repressing features.

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“…1,2 However, a sequential loss of lineage differentiation potential during culture of bone marrow stromal cells (BMSCs) that hinders their potential clinical application has been noted. 3 Unlike hematopoietic stem cells, no rigorous criteria have been developed for characterizing MSCs.…”

Section: Introductionmentioning

confidence: 99%

Clonal Isolation and Characterization of Bone Marrow Stromal Cells from Patients with Osteoarthritis

et al. 2007

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The demand for treatment strategies for damaged musculoskeletal tissue is continuously growing, especially with the increasing number of older people with degenerative diseases of the skeletal system such as osteoarthritis (OA). Because depletion of multipotent cells has been implicated in degenerative joint diseases, cell-based therapies have been proposed for tissue regeneration, especially for cartilage repair. The aim of the present study is to focus on the possibility of deriving and expanding multipotential mesenchymal stem cells (MSCs) from bone marrow samples of patients with OA by characterizing MSCs at the single cell level. Single-cell clonal cultures were established in 96-well plates by limiting dilution of bone marrow stromal cells (BMSCs) from three patients with OA. Fourteen clones were established for subsequent characterization. There was a wide variation in cell doubling times, with the time taken to reach 20 population doublings ranging from 37 days to more than 100 days. The clones were grouped into fast-growing and slow-growing clones. All except one of the fast-growing stem cell clones were tripotential. However, the slow-growing clones showed limited differentiation potential and morphological changes associated with cellular senescence with extended duration in culture. Flow cytometric analysis indicated a strong need to investigate for novel cell-surface characteristic markers of BMSCs because there was no obvious difference in the expression of the selected characteristic BMSC cell surface markers CD29, CD44, CD90, CD105, and CD166 between fast-growing and slow-growing clones. This study has demonstrated the existence of a fast-growing multipotential MSC population from bone marrow samples of patients with OA. Therefore, despite a supposedly smaller stem cell compartment in these patients, we demonstrate here that they can still yield a potentially therapeutically useful source of syngeneic MSCs.

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Repair of Experimental Alveolar Bone Defects by Tissue-Engineered Bone

Cited by 16 publications

References 24 publications

Repair of Rat Mandibular Bone Defects by Alveolar Osteoblasts in a Novel Plasma-Derived Albumin Scaffold

Repair of Rat Mandibular Bone Defects by Alveolar Osteoblasts in a Novel Plasma-Derived Albumin Scaffold

Crosstalk on Cell Behavior in Interactive Cocultures of hMSCs with Various Oral Cell Types

Clonal Isolation and Characterization of Bone Marrow Stromal Cells from Patients with Osteoarthritis

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