Repair of Critical-Sized Rat Calvarial Defects Using Genetically Engineered Bone Marrow-Derived Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor-1α

Zou, Duohong; Zhang, Zhiyuan; Ye, Dongxia; Tang, Aifa; Deng, Lianfu; Han, Wei; Zhao, Jun; Wang, Shuhong; Zhang, Wenjie; Zhu, Chao; Zhou, Ji; He, Jiacai; Wang, Yuanyin; Xu, Feng; Huang, Yadong; Jiang, Xinquan

doi:10.1002/stem.693

Cited by 100 publications

(69 citation statements)

References 35 publications

(34 reference statements)

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“…These MSCs contribute to tissue regeneration while forming new tissues by differentiation and secretion of various cytokines. 4,5 Stem cell-based therapy has been used to treat bone injury and may be one of the best approaches for bone regeneration. However, although cell transplantation results provide good prognosis, there are some obstacles such as cell source limitations, invasiveness, time requirements and the cost of expansion.…”

mentioning

confidence: 99%

Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering

Kim

Hur

Kim

et al. 2015

Tissue Engineering Part A

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mentioning

confidence: 99%

Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering

Kim

Hur

Kim

et al. 2015

Tissue Engineering Part A

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“…BMP-2 has in vivo osteoconductive activities compared to other BMP families. 18 BMP-2 expression in alveolar socket day on 7 after 6.35%, 12.7%, and 25.4% extract treatments showed the increase of BMP-2 compared to control group. This increase showed a faster bone formation through cellular mechanism.…”

Section: Resultsmentioning

confidence: 79%

The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction

Sukmana

Budhy

Ardani

2017

Dent. J. (Majalah Kedokteran Gigi)

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“…Previous studies Matsubara et al, 2005;Kretlow et al, 2008) showed that the decline in BMSC number and osteogenic differentiation capacity increased with age. In the present study, young (4 week old) male rats, which contain abundant bone marrow along with fragile femurs and tibiae, were used according to previous studies (Zou et al, 2011b;Zou et al, 2012).…”

Section: Discussion With Reviewersmentioning

confidence: 99%

Repair of critical-sized bone defects with anti-miR-31-expressing bone marrow stromal stem cells and poly(glycerol sebacate) scaffolds

Deng

Bi²,

Zhou³

et al. 2014

eCM

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The repair of critical-sized defects (CSDs) is a significant challenge in bone tissue engineering. Combining the use of progenitor cells with gene therapy represents a promising approach for bone regeneration. MicroRNAs play important roles in most gene regulatory networks, regulate the endogenous expression of multiple growth factors and simultaneously modulate stem cell differentiation. Our previous study showed that knocking down miR-31 promotes the osteogenesis of bone marrow stromal stem cells (BMSCs). To investigate the therapeutic potential of cells engineered to express anti-miR-31 for CSD repair, lentiviral vectors encoding negative control, miR-31 precursor and anti-sense sequences were constructed and transduced into osteo-inductive BMSCs. The expression of osteogenic-specific genes, alkaline phosphatase activity and Alizarin Red S staining were investigated to evaluate the effects of miR-31 on the cell fate of BMSCs over a 3-week period. In addition, miR-31-modified BMSCs seeded on poly(glycerol sebacate) (PGS) scaffolds were used to repair 8 mm critical-sized calvarial defects in rats. The results showed that miR-31 suppression significantly increased the expression of osteogenic-specific genes in vitro at the mRNA and protein levels, and that robust new bone formation with high local bone mineral density was observed in the anti-miR groups in vivo. Moreover, the PGS scaffolds carrying anti-miR-31-expressing BMSCs exhibited good biocompatibility and a high regeneration rate (~60 %) within in vivo bone defects. Our results suggest that miR-31 gene delivery affects the potential of BMSCs for osteogenic differentiation and bone regeneration and that PGS is a potential substrate for genetically modified, tissue-engineered bone in the repair of large bone defects.

show abstract

Repair of Critical-Sized Rat Calvarial Defects Using Genetically Engineered Bone Marrow-Derived Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor-1α

Abstract: The processes of angiogenesis and bone formation are coupled both temporally and spatially during bone repair. Bone marrow-derived mesenchymal stem cells (BMSCs) have been effectively used to heal critical-size bone defects.

Cited by 100 publications

References 35 publications

Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering

Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering

The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction

Repair of critical-sized bone defects with anti-miR-31-expressing bone marrow stromal stem cells and poly(glycerol sebacate) scaffolds

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