Repair of Bone Allograft Fracture Using Bone Morphogenetic Protein-2

Lee, Francis Young‐In; Storer, Stephen; Hazan, E; Gebhardt, Mark C.; Mankin, Henry J.

doi:10.1097/00003086-200204000-00017

Cited by 35 publications

(16 citation statements)

References 24 publications

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“…The study design was based on a rat femoral segmental defect model using an intramedullary K-wire for fixation. 12,13 A total of 46 rats were used to test four different groups. In the autograft group (n = 12), the mid-diaphysis of the femur was transected at two locations 5 mm apart, and the original host bone was maintained in the defect.…”

Section: In Vivo Animal Experimentsmentioning

confidence: 99%

“…To test our hypothesis, we used a rat femoral segmental defect model, which has been used previously to test the effects of various materials and agents on bone growth. [9][10][11][12][13][14] We created a 5 mm femoral defect, replaced the defect with appropriate grafts, and repaired the construct using an intramedullary K-wire. Segmental defects of 5 mm in the rat femurs have been shown to result consistently in nonunions, both radiographically and clinically.…”

Section: Introductionmentioning

confidence: 99%

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Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study

et al. 2013

IJN

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Background:To address the challenge of treating critical sized intercalary defects, we hypothesized that under physiologic cyclic loading, autografts, allografts, and scaffolds loaded with and without human mesenchymal stem cells (hMSCs) would have different biomechanical characteristics. Methods: Using a rat femoral defect model, 46 rats were assigned to four groups, ie, autograft (n = 12), allograft (n = 10), scaffold (n = 13), and scaffold with hMSCs (n = 11). The scaffold groups used a 5 mm segment of scaffold composed of 80% poly-ε-caprolactone and 20% hydroxyapatite. Rats were sacrificed 4 months postoperatively, and the repairs were assessed radiographically and biomechanically. Results: Autograft and allograft groups exhibited the most bridging callus, while the scaffold/ hMSCs group had more callus than the scaffold repairs. Although signs of radiographic healing did not accurately reflect restoration of mechanical properties, addition of hMSCs on the scaffold enhanced bone formation. The scaffold alone group had significantly lower elastic and viscous stiffness and higher phase angles than other repairs and the contralateral controls. Addition of hMSCs increased the elastic and viscous stiffness of the repair, while decreasing the phase angle. Conclusion: Further comparative analysis is needed to optimize clinical use of scaffolds and hMSCs for critical sized defect repairs. However, our results suggest that addition of hMSCs to scaffolds enhances mechanical simulation of native host bone.

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Section: In Vivo Animal Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study

et al. 2013

IJN

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“…For instance, the addition of bone morphogenetic proteins can promote a more extensive callus formation [13]. The use of viral vector-mediated gene therapy to enhance allograft remodelling has been reported [8].…”

Section: Discussionmentioning

confidence: 99%

Zoledronate reduces unwanted bone resorption in intercalary bone allografts

Seo

Cho

Storer

et al. 2009

International Orthopaedics (SICOT)

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Bone allografts are often hampered by graft incorporation and poor host bone formation. Bisphosphonates, synthetic pyrophosphate analogs, have shown promise in inhibiting bone resorption in human and animal trials. Some in vitro studies have suggested that high dose bisphosphonate may also inhibit bone formation, leading to our hypothesis that an ideal dose of bisphosphonate in allografts could protect allografts from resorption. We transplanted intercalary allografts in to the segmental defect of the rat femurs after soaking each allograft in zoledronate solution (30 µM) and then analysed bone density of the allografts six to 12 weeks after transplantation. At six and 12 weeks, the bone mineral density was higher in the experimental group compared with the control group. Qualitative radiographic and histological analysis also revealed more allograft resorption in the control group than in the zoledronatetreated group. Our data indicate that pharmacological modification of intercalary allografts with zoledronate solution can decrease osteoclast-mediated allograft resorption.

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“…The translated BMPs in vitro have proved to be safe and efficacious in promoting and accelerating bone healing in animal models, clinical and preclinical research [3,[22][23][24]. One recent study demonstrated healing of an allograft osteotomy in animal models after augmentation with rhBMP-2 [19]. The purpose of our study was to investigate the effect of rhBMP-2 on onlay strut allograft healing and remodelling in a rabbit model.…”

Section: Introductionmentioning

confidence: 96%

Effects of rhBMP-2 on cortical strut allograft healing to the femur in revision total hip arthroplasties: an experimental study

Xiong

2006

International Orthopaedics (SICO

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We have studied the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) on cortical strut allograft healing and remodelling in revision hip arthroplasty. Thirty adult New Zealand rabbits underwent bilateral onlay allograft strut procedures to the femur using wires. The left femur (experimental side) received the rhBMP-2 device (1.0-mg rhBMP-2/gelatin composites) interposed between the allograft and host bone, while the right side was grafted with an allograft strut as the control. The femurs and implants were retrieved at 4, 6, and 8 weeks postoperatively. The healing of cortical strut grafts to the femur was enhanced dramatically by the addition of the rhBMP-2 device in radiographic examination, contact radiographic examination, non-decalcification sections, fluorescence tag, and computer-aided image analysis. The remodelling of cortical strut allograft was also accelerated. The new bone formation ratio and radiographic scores of the experimental side were also much higher than the control side at all times. Strut healing with the rhBMP-2 device at 4 weeks postoperatively was superior to the healing in control sides at 8 weeks. Our findings showed that the rhBMP-2 device improved and accelerated the course of cortical strut allograft healing and remodelling with host bone.Résumé Nous avons étudié les effets de la BMP recombinante rhBMP-2 sur des allogreffes corticales dans les révisions de prothèses totales. 30 lapins adultes de Nouvelle Zélande ont bénéficié d'une allogreffe bilatérale sur un fémur cerclé avec interposition entre l'allogreffe et l'os receveur au niveau du fémur gauche de rhBMP-2 (1.0 mg rhBMP-2 de gélatines composites), le fémur droit étant greffé avec une allogreffe simple (groupe contrôle). Les fémurs et les implants ont été prélevés à 4, 6, 8 semaines post opératoires. La consolidation par allogreffe corticale a été considérablement améliorée par l'addition de rhBMP-2, tant sur le plan radiographique que sur le plan histologique. La consolidation avec rhBMP-2 à 4 semaines post opératoires est supérieure au groupe contrôle à 8 semaines. Notre expérimentation nous permet de montrer que le rhBMP-2 améliore la consolidation et le remodelage au niveau de l'os receveur des allogreffes corticales.

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Repair of Bone Allograft Fracture Using Bone Morphogenetic Protein-2

Cited by 35 publications

References 24 publications

Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study

Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study

Zoledronate reduces unwanted bone resorption in intercalary bone allografts

Effects of rhBMP-2 on cortical strut allograft healing to the femur in revision total hip arthroplasties: an experimental study

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