Repair characteristics and time-dependent effects in Saccharomyces cerevisiae cells after X-ray irradiation

Guo, Xiaopeng; Zhang, Miaomiao; Liu, Ruiyuan; Gao, Yuxi; Yang, Yang; Li, Wenjian; Dong, Lü

doi:10.1007/s11274-018-2566-9

Cited by 32 publications

(5 citation statements)

References 42 publications

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“…This includes the genes encoding glucokinase GLX1 , pyruvate kinase PYK2 , hexokinase HXK1 and glyceraldehyde-3-phosphate dehydrogenase TDH1 . It has previously been reported that yeast metabolism can be affected by various types of radiation treatment, but can also reflect the environmental stress response of yeast activated after various kinds of stresses [ 12 , 36 , 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae

Vanderwaeren

Dok

Voordeckers

et al. 2022

IJMS

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Proton radiotherapy (PRT) has the potential to reduce the normal tissue toxicity associated with conventional photon-based radiotherapy (X-ray therapy, XRT) because the active dose can be more directly targeted to a tumor. Although this dosimetric advantage of PRT is well known, the molecular mechanisms affected by PRT remain largely elusive. Here, we combined the molecular toolbox of the eukaryotic model Saccharomyces cerevisiae with a systems biology approach to investigate the physiological effects of PRT compared to XRT. Our data show that the DNA damage response and protein stress response are the major molecular mechanisms activated after both PRT and XRT. However, RNA-Seq revealed that PRT treatment evoked a stronger activation of genes involved in the response to proteotoxic stress, highlighting the molecular differences between PRT and XRT. Moreover, inhibition of the proteasome resulted in decreased survival in combination with PRT compared to XRT, not only further confirming that protons induced a stronger proteotoxic stress response, but also hinting at the potential of using proteasome inhibitors in combination with proton radiotherapy in clinical settings.

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Section: Resultsmentioning

confidence: 99%

An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae

Vanderwaeren

Dok

Voordeckers

et al. 2022

IJMS

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“…The Candida yeast species are more radio-resistant compared to other yeast cells such as for example Saccharomyces cerevisiae . For C. guilliermondii , the LD50 (semi-lethal dose) value for X-rays is about 720 Gy ( Navasardyan et al, 2017 ), and for S. cerevisiae 300 Gy ( Navasardyan et al, 2017 ) or even 60 Gy ( Guo et al, 2018a ). The radiation-induced decrease in the percentage of surviving cells leads to the lengthening of the lag phase of the growth of X-ray-irradiated yeast.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the nanoscale motion rate of Candida albicans by X-rays

Стародубцева

Челнокова²,

Shkliarava³

et al. 2023

Front. Microbiol.

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IntroductionPatients undergoing cancer treatment by radiation therapy commonly develop Candida albicans infections (candidiasis). Such infections are generally treated by antifungals that unfortunately also induce numerous secondary effects in the patient. Additional to the effect on the immune system, ionizing radiation influences the vital activity of C. albicans cells themselves; however, the reaction of C. albicans to ionizing radiation acting simultaneously with antifungals is much less well documented. In this study, we explored the effects of ionizing radiation and an antifungal drug and their combined effect on C. albicans.MethodsThe study essentially relied on a novel technique, referred to as optical nanomotion detection (ONMD) that monitors the viability and metabolic activity of the yeast cells in a label and attachment-free manner.Results and discussionOur findings demonstrate that after exposure to X-ray radiation alone or in combination with fluconazole, low-frequency nanoscale oscillations of whole cells are suppressed and the nanomotion rate depends on the phase of the cell cycle, absorbed dose, fluconazole concentration, and post-irradiation period. In a further development, the ONMD method can help in rapidly determining the sensitivity of C. albicans to antifungals and the individual concentration of antifungals in cancer patients undergoing radiation therapy.

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“…In this report, it was pointed to the enrichment of DEGs in detoxification and antioxidation activity categories of gene ontology at 1 hour. 22 Guintini et al studied the repair of telomeres after UV radiation on S. cerevisiae. 23 We highlighted telomeres as the targeted parts of S. cerevisiae in the previous investigations (In press data).…”

Section: Discussionmentioning

confidence: 99%

Exploring the Reversible Effects of UV Laser Radiation on the Gene Expression Profiles of Saccharomyces cerevisiae Via Network Analysis

et al. 2021

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Introduction: The reversibility of biological processes is an important challenge in the study of environmental pollutants and also natural and artificial radiation. There are many pieces of evidence about the reversible and irreversible effects of UV radiation on the human body. Assessment of the reversibility of UV laser effects on Saccharomyces cerevisiae was the aim of this study. Methods: Gene expression alteration in S. cerevisiae samples radiated by a 30s UV laser for 15, 30, and 60 minutes post-radiation times were investigated via network analysis to explore time-dependent reversible alteration in the gene expression profiles of the samples. Results: 19 differentially expressed genes (DEGs) were identified as targeted genes for the samples which were harvested 60 minutes after radiation; network analysis revealed no significant alteration in biological processes. Conclusion: It can be concluded that the gross effects of the UV laser on S. cerevisiae samples disappear after 60 minutes of radiation.

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Repair characteristics and time-dependent effects in Saccharomyces cerevisiae cells after X-ray irradiation

Cited by 32 publications

References 42 publications

An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae

An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae

Modulation of the nanoscale motion rate of Candida albicans by X-rays

Exploring the Reversible Effects of UV Laser Radiation on the Gene Expression Profiles of Saccharomyces cerevisiae Via Network Analysis

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