Repair and regeneration of lumbosacral nerve defects in rats with chitosan conduits containing bone marrow mesenchymal stem cells

Zhu, Lei; Liu, Tao; Cai, Jiao; Ma, Jun; Chen, Aimin

doi:10.1016/j.injury.2015.08.035

Cited by 15 publications

(9 citation statements)

References 34 publications

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“…Cell adhesion to the structure is determined by the extent of its positive charge, itself a function of the degree of alkaline deacetylation [1,24]. This effect has been reported for a number of anchorage-dependent cells, such as dorsal root ganglion neurons, Schwann cells [86], neural stem cells [109] and mesenchymal stem cells [110][111][112]. Examples for impregnating the nerve conduit wall with neurite outgrowth promoting factors and neurotrophins include the use of laminin [113,114], neurotrophin-3 [115] and transforming growth factor-β1 [116].…”

Section: Chitosan As An Artificial Nerve Graft Scaffold Fulfills Requmentioning

confidence: 89%

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Amr¹,

Ekram²

2017

Biological Activities and Application of Marine Polysaccharides

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Marine polysaccharides (e.g., chitosan, alginate and agarose) meet the requirements of artificial nerve grafting. These include the following: (1) prerequisites of biopolymers used as scaffolds; (2) conditions required by nerve autografts; (3) macroengineering requirements (form, design); (4) microengineering requirements (microgrooves, inclusion filaments); (5) mechanical conditions required by nerve autografts; (6) molecular aspects of peripheral nerve regeneration; space and adherence for: (i) chondroitin sulfate proteoglycans, (ii) neurite outgrowth promoting factors, (iii) neurotrophic factors, (iv) cells; (7) artificial side grafts should be compatible with autologous nerve grafts; (8) spatial distribution of neurotrophic factor gradients; (9) modulation of fibrosis; (10) renewal of luminal fillers. The mechanical stability of chitosan should be increased by adding other polymeric chains and cross-linking. As a result of deacetylation of chitin, chitosan scaffolds have got numerous positively charged free amino groups that provide adherence for growth factors and cells. To modulate fibrosis, heparin cross-linked chitosan microspheres have proved effective for delivering/transplanting cells, heparin and growth factors. To renew luminal fillers, chitosan microspheres may be injected through an indwelling catheter incorporated into the nerve conduit. Agarose and alginate have gained more acceptance as hydrogel lumen fillers. Interacting with positively charged chitosan, negatively charged alginate may form versatile chitosan-calcium-alginate microspheres.

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Section: Chitosan As An Artificial Nerve Graft Scaffold Fulfills Requmentioning

confidence: 89%

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Amr¹,

Ekram²

2017

Biological Activities and Application of Marine Polysaccharides

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“…Improved nerve regeneration was also reported for chitosan/poly(lactic-co-glycolic acid) scaffolds seeded with autologous bone marrow mesenchymal stem cells to treat injuries of dog sciatic nerves and rhesus monkey median nerves (Xue et al 2012;Hu et al 2013). In another approach, Zhu et al (2015) used chitosan conduits filled with bone marrow mesenchymal stem cells and evaluated nerve regeneration and neuronal survival when injured lumbosacral nerves were bridged with this material. They found that this treatment enhanced sacral nerve regeneration and motor function 6 and 12 weeks after the surgery.…”

Section: Neuronal Regenerationmentioning

confidence: 86%

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

Merzendorfer

Cohen

2019

Biologically-Inspired Systems

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Chitin is a linear polysaccharide of N-acetylglucosamine, which is highly abundant in nature and mainly produced by marine crustaceans. Chitosan is obtained by hydrolytic deacetylation. Both polysaccharides are renewable resources, simply and cost-effectively extracted from waste material of fish industry, mainly crab and shrimp shells. Research over the past five decades has revealed that chitosan, in particular, possesses unique and useful characteristics such as chemical versatility, polyelectrolyte properties, gel-and film-forming ability, high adsorption capacity, antimicrobial and antioxidative properties, low toxicity, and biocompatibility and biodegradability features. A plethora of chemical chitosan derivatives have been synthesized yielding improved materials with suggested or effective applications in water treatment, biosensor engineering, agriculture, food processing and storage, textile additives, cosmetics fabrication, and in veterinary and human medicine. The number of studies in this research field has exploded particularly during the last two decades. Here, we review recent advances in utilizing chitosan and chitosan derivatives in different technical, agricultural, and biomedical fields.

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“…Walking track analysis was performed on rats at 1 week after surgery, and the SFI was calculated according to our previously described methods. 64 …”

Section: Methodsmentioning

confidence: 99%

Silencing of circRNA.2837 Plays a Protective Role in Sciatic Nerve Injury by Sponging the miR-34 Family via Regulating Neuronal Autophagy

Zhou

Niu

Huang

et al. 2018

Molecular Therapy - Nucleic Acids

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Circular RNAs (circRNAs) represent a class of non-coding RNAs that are involved in transcriptional and posttranscriptional gene expression regulation and associated with different kinds of human diseases. However, the characterization and function of circular RNAs in peripheral nerve injuries remain elusive. Here, we established a rat sciatic nerve injury model and identified at least 4,942 distinct circular RNA candidates and a series of circular RNAs that were differentially expressed in injured nerve tissues compared with matched normal tissues. We characterized one frequently downregulated circular RNA, circRNA.2837, and further investigated its function in sciatic nerve injury. We found that circRNA.2837 regulated autophagy in neurons in vitro and in vivo, and downregulation of circRNA.2837 alleviated sciatic nerve injury via inducing autophagy in vivo. Mechanistically, knockdown of circRNA.2837 may protect neurons against neurological injury by acting as a sponge for members of miR-34 family. Our findings suggested that differentially expressed circular RNAs were involved in the pathogenesis of sciatic nerve injury, and circular RNAs exerted regulatory functions in sciatic nerve injury and might be used as potential targets in sciatic nerve injury therapy.

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Repair and regeneration of lumbosacral nerve defects in rats with chitosan conduits containing bone marrow mesenchymal stem cells

Cited by 15 publications

References 34 publications

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

Silencing of circRNA.2837 Plays a Protective Role in Sciatic Nerve Injury by Sponging the miR-34 Family via Regulating Neuronal Autophagy

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