REP-PCR tracking of the origin and spread of airborne Staphylococcus aureus in and around chicken house

Zhong, Zhijun; Chai, Tongjie; Duan, Haibao; Miao, Zengmin; Li, X.; Yao, Meiling; Yuan, Wen; Wang, W.; Li, Q.; Zucker, Barbara; Schlenker, G

doi:10.1111/j.1600-0668.2009.00618.x

Cited by 23 publications

(19 citation statements)

References 26 publications

(26 reference statements)

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“…In addition, airborne spread of S. aureus has been demonstrated in hospitals (Gehanno et al, 2009;Hsiao et al, 2012;Mirzaii et al, 2012;Shiomori et al, 2001Shiomori et al, , 2002, residences (Davis et al, 2012;Gandara et al, 2006;Perez et al, 2011), and livestock-housing barns (Friese et al, , 2013Schulz et al, 2012;Zhong et al, 2009), highlighting the importance of S. aureus transmission via airborne routes (Friese et al, , 2013Shiomori et al, 2001Shiomori et al, , 2002. In addition, airborne spread of S. aureus has been demonstrated in hospitals (Gehanno et al, 2009;Hsiao et al, 2012;Mirzaii et al, 2012;Shiomori et al, 2001Shiomori et al, , 2002, residences (Davis et al, 2012;Gandara et al, 2006;Perez et al, 2011), and livestock-housing barns (Friese et al, , 2013Schulz et al, 2012;Zhong et al, 2009), highlighting the importance of S. aureus transmission via airborne routes (Friese et al, , 2013Shiomori et al, 2001Shiomori et al, , 2002.…”

Section: Introductionmentioning

confidence: 99%

“…Airborne S. aureus has been collected by agar impaction with the Andersen one-stage sampler (Andersen 1-STG) (Gandara et al, 2006;Hsiao et al, 2012;Huang et al, 2013;Mirzaii et al, 2012;Perez et al, 2011;Zhong et al, 2009) and by liquid impingement in the all-glass impinger (AGI-30) (Friese et al, , 2013Schulz et al, 2012). It is noted that the sampling with liquid collection allows a longer sampling time than the agar impaction method, which is important for the assessment of environmental S. aureus aerosols as it increases the volume of air sampled and thereby decreases the lower limit of detection and minimizes the false-negative result.…”

Section: Introductionmentioning

confidence: 99%

“…Staphylococcus aureus may be transmitted through person-to-person contact. In addition, airborne spread of S. aureus has been demonstrated in hospitals (Gehanno et al, 2009;Hsiao et al, 2012;Mirzaii et al, 2012;Shiomori et al, 2001Shiomori et al, , 2002, residences (Davis et al, 2012;Gandara et al, 2006;Perez et al, 2011), and livestock-housing barns (Friese et al, , 2013Schulz et al, 2012;Zhong et al, 2009), highlighting the importance of S. aureus transmission via airborne routes (Friese et al, , 2013Shiomori et al, 2001Shiomori et al, , 2002. Indeed, the prevention of airborne transmission of S. aureus has been addressed in many studies (Gehanno et al, 2009;Mirzaii et al, 2012;Schulz et al, 2012;Shiomori et al, 2001Shiomori et al, , 2002 and in the Dutch guideline for nosocomial infection controls (Bernards et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Methods for quantifyingStaphylococcus aureusin indoor air

Chang

Wang

2014

Indoor Air

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Staphylococcus aureus has been detected in indoor air and linked to human infection. Quantifying S. aureus by efficient sampling methods followed by appropriate sample storage treatments is essential to characterize the exposure risk of humans. This laboratory study evaluated the effects of sampler type (all-glass impinger (AGI-30), BioSampler, and Andersen one-stage sampler (Andersen 1-STG)), collection fluid (deionized water (DW), phosphate-buffered saline (PBS), and Tween mixture (TM)), and sampling time (3-60 min) on cell recovery. Effects of storage settings on bacterial concentration were also assessed over 48 h. Results showed BioSampler performed better than Andersen 1-STG and AGI-30 (P < 0.05) and TM was superior to PBS and DW (P < 0.05). An increase in sampling time negatively affected the recoveries of cells in PBS of BioSampler and AGI-30 (P < 0.05), whereas cell recoveries in TM were increased at sampling of 6-15 min compared with 3 min. Concentrations of cells collected in PBS were decreased with storage time at 4 and 23 °C (P < 0.05), while cells stored in TM showed stable concentrations at 4 °C (P > 0.05) and increased cell counts at 23 °C (P < 0.05). Overall, sampling by BioSampler with TM followed by sample transportation and storage at 4 °C is recommended.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Methods for quantifyingStaphylococcus aureusin indoor air

Chang

Wang

2014

Indoor Air

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“…Caroll et al (2016) menyatakan beberapa mekanisme yang menyebabkan bakteri resisten terhadap obat-obatan, antara lain : 1) bakteri memproduksi enzim yang menghancurkan zat aktif yang terdapat dalam obat, 2) bakteri mengubah permeabilitasnya terhadap obat , 3) bakt eri mengembangkan struktur target lain untuk obat, 4) bakteri mengembangkan jalur metabolisme yang terlewati saat terjadi reaksi penghambatan oleh obat, dan 5) bakteri mengembangkan enzim lain yang tetap dapat menjalankan fungsi metabolisnya namun kurang dipengaruhi oleh obat. Dalam hal ini bakteri yang resisten dapat tersebar melalui udara (Brooks, McLaughlin, Schefffler, & Miles, 2010;Gibbs et al, 2006;McEachran, 2015;Rule, Evans, & Silbergeld, 2008;Zhong, Chai, & Duan, 2009), air (Wilson, 2004) dan angin (Marti et al, 2014;Wichmann, UdikovicKolic, Andrew, Handelsman, 2014). Bahkan serangga (Marti, 2013) dan tikus (Literak et al, 2009;van de Giessen et al, 2009) dapat membawa bakteri resisten dari lokasi pertanian.…”

Section: Pola Resistensi Bakteri Terhadap Antibiotikunclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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“…Staphylococcus aureus , a gram‐positive bacterium, has been detected in the air of various environments including residences (Davis et al., ; Gandara et al., ; Perez et al., ), hospitals (Gehanno et al., ; Hsiao et al., ; Mirzaii et al., ; Shiomori et al., , ), and livestock‐housing facilities (Friese et al., , ; Schulz et al., ; Zhong et al., ). Exposure to S. aureus may lead to various human diseases from mild skin infections to lethal pneumonia, sepsis, and toxic shock syndrome (Weber, ).…”

Section: Introductionmentioning

confidence: 99%

Impact of culture media and sampling methods onStaphylococcus aureusaerosols

Chang

Wang

2014

Indoor Air

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Staphylococcus aureus has been detected indoors and is associated with human infection. Reliable quantification of S. aureus using a sampling technique followed by culture assay helps in assessing the risks of human exposure. The efficiency of five culture media and eight sampling methods in recovering S. aureus aerosols were evaluated. Methods to extract cells from filters were also studied. Tryptic soy agar (TSA) presented greater bacterial recovery than mannitol salt agar (MSA), CHROMagar staph aureus, Chapman stone medium, and Baird-Park agarose (P < 0.05). Moreover, 93 ± 2%-95 ± 2% and 42 ± 1%-49 ± 2% of S. aureus were, respectively, recovered by a 15-min heating of gelatin filters and 2-min vortex of polycarbonate (PC) filters. Evaluation of two filtration (IOM with gelatin filter and cassette with PC filter), two impaction (Andersen 1-STG loaded with TSA and MSA) and four impingement methods [AGI-30 and BioSampler filled with Tween mixture (TM) and phosphate-buffered saline (PBS)] revealed the BioSampler/TM performed best over 30 and 60 min of sampling (P < 0.05), while low recovery efficiencies were associated with the IOM/gelatin, cassette/PC, and AGI-30/PBS combinations (P < 0.05). In addition to BioSampler/TM, collecting S. aureus onto TSA from the Andersen 1-STG is also recommended, as it is the second best method at the 60-min sampling (P < 0.05).

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REP-PCR tracking of the origin and spread of airborne Staphylococcus aureus in and around chicken house

Cited by 23 publications

References 26 publications

Methods for quantifyingStaphylococcus aureusin indoor air

Methods for quantifyingStaphylococcus aureusin indoor air

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Impact of culture media and sampling methods onStaphylococcus aureusaerosols

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