Reoxygenation Injury following Anoxic Perfusion Preferentially Impairs Bile Acid-Independent Bile Flow

Konno, Hiroyuki; Hardison, W. G.; Miyai, Katsumi

doi:10.1159/000129147

Cited by 6 publications

(1 citation statement)

References 2 publications

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“…Ischemia/ reperfusion injury impairs preferentially bile acid-inde pendent bile flow. 51 To characterize postischemic bile formation in more detail, we quantified the biliary excre tion of actively transported GSH 20,21 and GSSG, 18,19 which partially contribute to the bile acid-independent bile secretion in rat liver. 52 The biliary release of total glutathione and GSSG was markedly decreased during the reflow period.…”

Section: Postischemic Bile Flow and Biliary Glutathione Transportmentioning

confidence: 99%

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Bilzer¹,

Witthaut²,

Paumgartner³

et al. 1994

Gastroenterology

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Section: Postischemic Bile Flow and Biliary Glutathione Transportmentioning

confidence: 99%

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Bilzer¹,

Witthaut²,

Paumgartner³

et al. 1994

Gastroenterology

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Differential impact of carolina rinse and university of wisconsin solutions on microcirculation, leukocyte adhesion, kupffer cell activity and biliary excretion after liver transplantation

et al. 1993

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This quantitative in vivo fluorescence microscopy study investigated the relative impact of an optimized rinse solution (warm Carolina rinse) and that of an established storage solution (University of Wisconsin solution) on various pathomechanisms of hepatic reperfusion injury after cold storage. Syngeneic orthotopic, arterialized liver transplantation was performed in male Lewis rats after 24 hr of cold ischemia (n = 24). The four experimental groups differed according to the type of preservation/rinse solution used: University of Wisconsin solution/albumin rinse (group 1), autologous blood (just external cooling)/albumin rinse (group 2), blood/Carolina rinse (group 3) and University of Wisconsin solution/Carolina rinse (group 4). Hepatic microvascular perfusion, leukocyte accumulation and phagocytic activity of Kupffer cells were assessed by means of intravital fluorescence microscopy 30 to 90 min after reperfusion. Disturbances of microvascular perfusion were most pronounced in group 2, markedly reduced by University of Wisconsin solution (group 1) and Carolina rinse (group 3) and minimized by combined use of University of Wisconsin solution and Carolina rinse in group 4. Intrahepatic leukocyte-endothelium interaction in sinusoids and postsinusoidal venules was found to depend on the application of Carolina rinse before reperfusion rather than the use of University of Wisconsin solution during cold storage. Activation of phagocytosis by Kupffer cells was most pronounced in group 1, intermediate in groups 2 and 3 and not noticeable in group 4. Hepatocellular excretory function as assessed on the basis of total bile flow and excretion of bile acids during the first 90 min after reperfusion was found to be improved by application of Carolina rinse, both after storage in blood or in University of Wisconsin solution.(ABSTRACT TRUNCATED AT 250 WORDS)

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Intravital studies on beneficial effects of warm Ringer's lactate rinse in liver transplantation

Rentsch

Post

Palma³

et al. 1996

Transpl Int

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This quantitative in vivo fluorescence microscopy study investigated the impact of warm versus cold Ringer's lactate (RL) graft rinse on various microvascular manifestations of ischemia-reperfusion injury after liver transplantation in the rat. Syngeneic orthotopic liver transplantation, including arterial revascularization, was performed in male Lewis rats following 24 h of cold storage in University of Wisconsin (UW) solution. In one group (n = 8) liver grafts were rinsed with 4 degrees C (cold) RL, whereas in the other group (n = 8) grafts were rinsed with 37 degrees C (warm) RL immediately prior to revascularization. Hepatic microvascular perfusion, leukocyteendothelium interaction, and Kupffer cell activation were quantified 30-90 min after graft reperfusion by direct visualization with intravital fluorescence microscopy. Moreover, biliary excretory graft function was analyzed by determination of bile flow and bile salt excretion during the first 90 min after reperfusion. Compared to grafts rinsed with cold RL, acinar and sinusoidal perfusion were found to be significantly increased after rinsing the grafts with warm RL. The amount of nonperfused acini declined from 18.1% +/- 4.0% to 7.4% +/- 1.6% (P < 0.05), and the total percentage of perfused sinusoids increased from 80.1 +/- 1.4 to 88.4 +/- 1.2 (P < 0.001) after cold and warm rinse, respectively. After rinsing the graft with warm RL, WBC adherence in sinusoids and especially in postsinusoidal venules decreased significantly by 28% (P < 0.001) and 33% (P < 0.001), respectively. Kupffer cell activation was markedly reduced after rinsing with RL at 37 degrees C, as indicated by a decelerated adherence of latex particles injected 80 min after reperfusion. Excretory graft function was dramatically increased following warm RL rinse during the 90-min observation period. Bile flow was enhanced from 1.04 +/- 0.5 to 3.9 +/- 0.8 ml/100 g liver per 90 min (P < 0.01), with a parallel rise in bile salt excretion from 24.3 +/- 5.8 to 128.0 +/- 19.8 mmol/ 100 g liver per 90 min (P < 0.05) when compared to cold RL. These data strongly suggest that rinsing liver grafts with warm RL prior to reperfusion represents a simple and inexpensive way to reduce the incidence of primary graft failure secondary to ischemia and reperfusion injury in liver transplantation.

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Reoxygenation Injury following Anoxic Perfusion Preferentially Impairs Bile Acid-Independent Bile Flow

Cited by 6 publications

References 2 publications

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Differential impact of carolina rinse and university of wisconsin solutions on microcirculation, leukocyte adhesion, kupffer cell activity and biliary excretion after liver transplantation

Intravital studies on beneficial effects of warm Ringer's lactate rinse in liver transplantation

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