Reorganization of CA1 dendritic dynamics by hippocampal sharp-wave ripples during learning

Rolotti, Sebi V.; Blockus, Heike; Sparks, Fraser T.; Priestley, James B.; Losonczy, Attila

doi:10.1016/j.neuron.2021.12.017

Cited by 32 publications

(23 citation statements)

References 141 publications

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“…Acute Pdzd8 deletion as a tool to investigate the role of ICR Given the absence of in vivo functional imaging data for apical CA1PN dendrites and the paucity of such data for basal dendrites (41)(42)(43), we first characterized spontaneous Ca 2+ transient properties in apical and basal dendrites with respect to their parent soma and assessed any impact of our ICR-augmenting manipulation on basic CA1PN activity dynamics. Pdzd8 KO and WT CA1PN cell bodies did not differ in Ca 2+ transient frequency or amplitude (fig.…”

Section: Single-cell Genetic Deletion and In Vivo Subcellular Imaging...mentioning

confidence: 99%

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release

O’Hare

Gonzalez

Herrlinger

et al. 2022

Science

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Dendritic calcium signaling is central to neural plasticity mechanisms that allow animals to adapt to the environment. Intracellular calcium release (ICR) from the endoplasmic reticulum has long been thought to shape these mechanisms. However, ICR has not been investigated in mammalian neurons in vivo. We combined electroporation of single CA1 pyramidal neurons, simultaneous imaging of dendritic and somatic activity during spatial navigation, optogenetic place field induction, and acute genetic augmentation of ICR cytosolic impact to reveal that ICR supports the establishment of dendritic feature selectivity and shapes integrative properties determining output-level receptive fields. This role for ICR was more prominent in apical than in basal dendrites. Thus, ICR cooperates with circuit-level architecture in vivo to promote the emergence of behaviorally relevant plasticity in a compartment-specific manner.

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Section: Single-cell Genetic Deletion and In Vivo Subcellular Imaging...mentioning

confidence: 99%

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release

O’Hare

Gonzalez

Herrlinger

et al. 2022

Science

Self Cite

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“…Indeed, multiphoton imaging is limited to a depth of several hundred microns, necessitating tissue removal or insertion of an invasive lens to gain access to deeper brain structures. [28][29][30] Recent developments in three-photon imaging have extended the accessible depths, but availability of fluorophores and laser sources compatible with this modality remain limited. 31 Finally, the FOV accessible with point-scanning is limited, with most multiphoton systems providing <1 mm 2 .…”

Section: Multiphoton Imagingmentioning

confidence: 99%

Building bridges: simultaneous multimodal neuroimaging approaches for exploring the organization of brain networks

Lake

Higley

2022

Neurophoton.

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Brain organization is evident across spatiotemporal scales as well as from structural and functional data. Yet, translating from micro-to macroscale (vice versa) as well as between different measures is difficult. Reconciling disparate observations from different modes is challenging because each specializes within a restricted spatiotemporal milieu, usually has bounded organ coverage, and has access to different contrasts. True intersubject biological heterogeneity, variation in experiment implementation (e.g., use of anesthesia), and true moment-to-moment variations in brain activity (maybe attributable to different brain states) also contribute to variability between studies. Ultimately, for a deeper and more actionable understanding of brain organization, an ability to translate across scales, measures, and species is needed. Simultaneous multimodal methods can contribute to bettering this understanding. We consider four modes, three optically based: multiphoton imaging, single-photon (wide-field) imaging, and fiber photometry, as well as magnetic resonance imaging. We discuss each mode as well as their pairwise combinations with regard to the definition and study of brain networks.

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“…Including opsin-coding genes in the vector plasmids allows the expression of opsins in specific neurons. This technique enables optical control of neuronal activity [ 88 , 89 ] and imaging of neuronal populations [ 90 , 91 , 92 ]. Here, we describe how in utero electroporation has been used to scrutinize neural circuitry, neural pathways, and animal behavior.…”

Section: Combining In Utero Electroporation and Optogeneticsmentioning

confidence: 99%

In Utero Electroporation for Manipulation of Specific Neuronal Populations

2022

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The complexity of brain functions is supported by the heterogeneity of brain tissue and millisecond-scale information processing. Understanding how complex neural circuits control animal behavior requires the precise manipulation of specific neuronal subtypes at high spatiotemporal resolution. In utero electroporation, when combined with optogenetics, is a powerful method for precisely controlling the activity of specific neurons. Optogenetics allows for the control of cellular membrane potentials through light-sensitive ion channels artificially expressed in the plasma membrane of neurons. Here, we first review the basic mechanisms and characteristics of in utero electroporation. Then, we discuss recent applications of in utero electroporation combined with optogenetics to investigate the functions and characteristics of specific regions, layers, and cell types. These techniques will pave the way for further advances in understanding the complex neuronal and circuit mechanisms that underlie behavioral outputs.

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Reorganization of CA1 dendritic dynamics by hippocampal sharp-wave ripples during learning

Cited by 32 publications

References 141 publications

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release

Building bridges: simultaneous multimodal neuroimaging approaches for exploring the organization of brain networks

In Utero Electroporation for Manipulation of Specific Neuronal Populations

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Reorganization of CA1 dendritic dynamics by hippocampal sharp-wave ripples during learning

Cited by 32 publications

References 141 publications

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca 2+ release

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca 2+ release

Building bridges: simultaneous multimodal neuroimaging approaches for exploring the organization of brain networks

In Utero Electroporation for Manipulation of Specific Neuronal Populations

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Product

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Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release

Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release