Reorganization and in Vivo Dynamics of Microtubules during Arabidopsis Root Hair Development

Bruaene, Nicholas Van; Joss, Greg; Oostveldt, Patrick Van

doi:10.1104/pp.103.031591

Cited by 81 publications

(98 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A projection of several optical sections from a wild-type root hair expressing GFP-MBD showed predominantly longitudinal microtubules, consistent with a previous report (Van Bruaene et al, 2004). On the other hand, root hairs of agd1-1 that displayed regular wavy growth accumulated thick microtubule bundles along their length (Fig.…”

Section: Identification and Characterization Of Arabidopsis Mutants Wsupporting

confidence: 77%

“…3; Supplemental Movie S6; Sakai et al, 2008). In Arabidopsis root hairs, endoplasmic microtubules have been proposed to function as microtubule nucleation complexes (Van Bruaene et al, 2004). Thus, ARK1 and AGD1 could conceivably be part of a signaling pathway that regulates microtubule nucleation, and their altered activity could result in abnormally high levels of polymerized tubulin at the root hair tip, leading to unstable growth.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Class I ADP-Ribosylation Factor GTPase-Activating Protein Is Critical for Maintaining Directional Root Hair Growth in Arabidopsis

et al. 2008

View full text Add to dashboard Cite

Membrane trafficking and cytoskeletal dynamics are important cellular processes that drive tip growth in root hairs. These processes interact with a multitude of signaling pathways that allow for the efficient transfer of information to specify the direction in which tip growth occurs. Here, we show that AGD1, a class I ADP ribosylation factor GTPase-activating protein, is important for maintaining straight growth in Arabidopsis (Arabidopsis thaliana) root hairs, since mutations in the AGD1 gene resulted in wavy root hair growth. Live cell imaging of growing agd1 root hairs revealed bundles of endoplasmic microtubules and actin filaments extending into the extreme tip. The wavy phenotype and pattern of cytoskeletal distribution in root hairs of agd1 partially resembled that of mutants in an armadillo repeat-containing kinesin (ARK1). Root hairs of double agd1 ark1 mutants were more severely deformed compared with single mutants. Organelle trafficking as revealed by a fluorescent Golgi marker was slightly inhibited, and Golgi stacks frequently protruded into the extreme root hair apex of agd1 mutants. Transient expression of green fluorescent protein-AGD1 in tobacco (Nicotiana tabacum) epidermal cells labeled punctate bodies that partially colocalized with the endocytic marker FM4-64, while ARK1-yellow fluorescent protein associated with microtubules. Brefeldin A rescued the phenotype of agd1, indicating that the altered activity of an AGD1-dependent ADP ribosylation factor contributes to the defective growth, organelle trafficking, and cytoskeletal organization of agd1 root hairs. We propose that AGD1, a regulator of membrane trafficking, and ARK1, a microtubule motor, are components of converging signaling pathways that affect cytoskeletal organization to specify growth orientation in Arabidopsis root hairs.

show abstract

Section: Identification and Characterization Of Arabidopsis Mutants Wsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

A Class I ADP-Ribosylation Factor GTPase-Activating Protein Is Critical for Maintaining Directional Root Hair Growth in Arabidopsis

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Different examples of spontaneous self-organization of dynamic microtubules have been reported in the literature [19,20,21,22,23]. One of them [20], describes the formation of spatial structures in the in-vitro solution of microtubules that start growing from seeds distributed homogeneously.…”

Section: Introductionmentioning

confidence: 99%

Collision induced spatial organization of microtubules

Baulin¹,

Marques²,

Thalmann³

2007

Biophysical Chemistry

View full text Add to dashboard Cite

The dynamic behavior of microtubules in solution can be strongly modified by interactions with walls or other structures. We examine here a microtubule growth model where the increase in size of the plus-end is perturbed by collisions with other microtubules. We show that such a simple mechanism of constrained growth can induce ordered structures and patterns from an initially isotropic and homogeneous suspension. First, microtubules self-organize locally in randomly oriented domains that grow and compete with each other. By imposing even a weak orientation bias, external forces like gravity or cellular boundaries may bias the domain distribution eventually leading to a macroscopic sample orientation.

show abstract

“…Cortical microtubules are sparse in the lobes of leaf epidermal cells (Fu et al, 2005) and are absent in the apex of growing pollen tubes (Yang, 2008) and root hairs (Van Bruaene et al, 2004). At the pollen tube apex, ROP GTPases are activated, and ROP INTERACTIVE PARTNER1 (RIP1)/INTERACTOR OF CONSTITUTIVE ACTIVE ROPS1 (ICR1), which belongs to the same family as MIDD1, is also localized (Li et al, 2008).…”

Section: The Rop11-midd1-cascade Regulates Kinesin-13a Localizationmentioning

confidence: 99%

Rho of Plant GTPase Signaling Regulates the Behavior of Arabidopsis Kinesin-13A to Establish Secondary Cell Wall Patterns

2013

View full text Add to dashboard Cite

Plant cortical microtubule arrays determine the cell wall deposition pattern and proper cell shape and function. Although various microtubule-associated proteins regulate the cortical microtubule array, the mechanisms underlying marked rearrangement of cortical microtubules during xylem differentiation are not fully understood. Here, we show that local Rho of Plant (ROP) GTPase signaling targets an Arabidopsis thaliana kinesin-13 protein, Kinesin-13A, to cortical microtubules to establish distinct patterns of secondary cell wall formation in xylem cells. Kinesin-13A was preferentially localized with cortical microtubules in secondary cell wall pits, areas where cortical microtubules are depolymerized to prevent cell wall deposition. This localization of Kinesin-13A required the presence of the activated ROP GTPase, MICROTUBULE DEPLETION DOMAIN1 (MIDD1) protein, and cortical microtubules. Knockdown of Kinesin-13A resulted in the formation of smaller secondary wall pits, while overexpression of Kinesin-13A enlarged their surface area. Kinesin-13A alone could depolymerize microtubules in vitro; however, both MIDD1 and Kinesin-13A were required for the depolymerization of cortical microtubules in vivo. These results indicate that Kinesin-13A regulates the formation of secondary wall pits by promoting cortical microtubule depolymerization via the ROP-MIDD1 pathway.

show abstract

Reorganization and in Vivo Dynamics of Microtubules during Arabidopsis Root Hair Development

Cited by 81 publications

References 76 publications

A Class I ADP-Ribosylation Factor GTPase-Activating Protein Is Critical for Maintaining Directional Root Hair Growth in Arabidopsis

A Class I ADP-Ribosylation Factor GTPase-Activating Protein Is Critical for Maintaining Directional Root Hair Growth in Arabidopsis

Collision induced spatial organization of microtubules

Rho of Plant GTPase Signaling Regulates the Behavior of Arabidopsis Kinesin-13A to Establish Secondary Cell Wall Patterns

Contact Info

Product

Resources

About