Renshen Shouwu extract enhances neurogenesis and angiogenesis via inhibition of TLR4/NF-κB/NLRP3 signaling pathway following ischemic stroke in rats

Li, Yuping; Liang, Wenyi; Guo, Caijuan; Chen, Xu; Huang, Yijian; Wang, Hong; Song, Lixia; Zhang, Dongxing; Zhan, Wenjing; Lin, Ziyang; Tan, Haibo; Bei, Weijian; Guo, Jiao

doi:10.1016/j.jep.2020.112616

Cited by 42 publications

(28 citation statements)

References 41 publications

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“…In vitro, ginsenosides is reported to activate phagocytosis on macrophage RAW264.7 cells through regulating TLR2/4 and NF-κB signaling pathways and the enhancement of TLR4 expression is also found (Xu et al, 2018;Gao et al, 2020). In vivo, Radix Ginseng is also reported to down-regulate the TLR4 expressions in disease-like animal models like cerebral Ischemia rats (Cheng et al, 2019;Li et al, 2020). These findings support that Radix Ginseng exert bi-directional function in regulating immunity by enhancing phagocytosis in normal condition while inhibiting abnormal stimulation of phagocytes in pathological condition.…”

Section: Discussionmentioning

confidence: 96%

Chinese Medicine Formula Kai-Xin-San Ameliorates Neuronal Inflammation of CUMS-Induced Depression-like Mice and Reduces the Expressions of Inflammatory Factors via Inhibiting TLR4/IKK/NF-κB Pathways on BV2 Cells

Liu

Cao

et al. 2021

Front. Pharmacol.

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Kai-Xin-San (KXS) is a traditional Chinese medicinal formula composed of Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria for relieving major depressive disorder and Alzheimer’s disease in traditional Chinese medicine (TCM) clinics. Previous studies on the antidepressant mechanism of KXS mainly focused on neurotransmitter and neurotrophic factor regulation, but few reports exist on neuronal inflammation regulation. In the current study, we found that KXS exerted antidepressant effects in chronic unpredictable mild stress-induced depression-like mice according to the results of behavioral tests. Meanwhile, KXS also inhibited the activation of microglia and significantly reduced the expression of pro-inflammatory cytokines such as IL-1β, IL−2, and TNF-α in the hippocampus of mice. In mice BV2 microglia cell lines, KXS extract reduced the expression of inflammatory factors in BV2 cells induced by lipopolysaccharide via inhibiting TLR4/IKK/NF-κB pathways, which was also validated by the treatment of signaling pathway inhibitors such as TAK-242 and JSH-23. T0hese data implied that the regulation of pro-inflammatory cytokines in microglia might account for the antidepressant effect of KXS, thereby providing more scientific information for the development of KXS as an alternative therapy for major depressive disorder.

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Section: Discussionmentioning

confidence: 96%

Chinese Medicine Formula Kai-Xin-San Ameliorates Neuronal Inflammation of CUMS-Induced Depression-like Mice and Reduces the Expressions of Inflammatory Factors via Inhibiting TLR4/IKK/NF-κB Pathways on BV2 Cells

Liu

Cao

et al. 2021

Front. Pharmacol.

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“…Emerging studies have revealed that NF- κ B proteins are a member of the transcription factor family which is stimulated by chemokine, extracellular matrix (ECM) degradation products, stress-related factors, and proinflammatory cytokines [ 36 ]. A recent study also suggested that the expression of the NLRP3 inflammasome was accompanied by the activation of Toll-like receptors (TLRs) and NF- κ B [ 37 ]. In the present study, we confirmed that NF- κ B inhibitor suppressed NLRP3 expression.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of LMX1B Suppressed Cell Apoptosis and Inflammatory Response in IL-1β-Induced Human Osteoarthritis Chondrocytes through NF-κB and NLRP3 Signal Pathway

Wang

et al. 2022

Mediators of Inflammation

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Osteoarthritis (OA), a chronic degenerative joint disease, always occurred in the aging population. There is evidence suggests that chondrocytes’ survival, inflammation, and apoptosis play critical roles in OA pathogenesis. LMX1B has been shown to be involved in antiosteogenic function in early patterning of the calvaria. However, the role and mechanism of LMX1B in OA is not unknown. The present study observed that LMX1B was highly expressed in OA patients compared with normal patients. Besides, we found that IL-1β increased LMX1B mRNA and protein expression in SW1353 and C28/I2 chondrocytes. LMX1B knockdown increased IL-1β-induced cell viability and proliferation and suppressed cell apoptosis and inflammation response, including IFN-γ, TNF-α, IL-6, prostaglandin E2 (PGE2), and NO both in SW1353 and C28/I2. Furthermore, LMX1B silence inhibited MMP-3 and MMP-13 expression both in SW1353 and C28/I2 cells. Also, the activation of the NF-κB and NLRP3 signaling pathway was suppressed in LMX1B silence cells by decreasing the p-p65 and NLRP3 protein expressions. Additionally, inhibition of NF-κB by PDTC suppressed NLRP3 expression. Moreover, NLRP3 overexpression reversed the effects of LMX1B silence on chondrocytes’ survival, proliferation, apoptosis, and inflammation. Finally, we confirmed that LMX1B depletion had protective effects in OA rats in vivo.

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“…Renshen Shouwu (RSSW), which is composed of P. ginseng and fleece flower root (Polygonum multiflorum Thunb. ), is a traditional Chinese medicine that is beneficial in the prevention of neurasthenia, forgetfulness, insomnia, inappetence, and excessive fatigue [ 94 ]. Research has shown that it increases neurogenesis (the number of newborn neurons) and angiogenesis (brain microvessel density) after ischemic stroke via inhibition of the TLR4/NF-κB/NLRP3 inflammatory signaling pathway (TLR4, p–NF-κB p65/p65, NLRP3, pro-IL-1β, IL-1β, pro-caspase-1, caspase-1 proteins) in MCAO-induced rats [ 94 ] ( Table 1 ).…”

Section: P Ginseng As a Potential Therapeutic For Neurologic...mentioning

confidence: 99%

Panax ginseng as a potential therapeutic for neurological disorders associated with COVID-19; Toward targeting inflammasome

Shin

Cho

2023

Journal of Ginseng Research

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Renshen Shouwu extract enhances neurogenesis and angiogenesis via inhibition of TLR4/NF-κB/NLRP3 signaling pathway following ischemic stroke in rats

Cited by 42 publications

References 41 publications

Chinese Medicine Formula Kai-Xin-San Ameliorates Neuronal Inflammation of CUMS-Induced Depression-like Mice and Reduces the Expressions of Inflammatory Factors via Inhibiting TLR4/IKK/NF-κB Pathways on BV2 Cells

Chinese Medicine Formula Kai-Xin-San Ameliorates Neuronal Inflammation of CUMS-Induced Depression-like Mice and Reduces the Expressions of Inflammatory Factors via Inhibiting TLR4/IKK/NF-κB Pathways on BV2 Cells

Knockdown of LMX1B Suppressed Cell Apoptosis and Inflammatory Response in IL-1β-Induced Human Osteoarthritis Chondrocytes through NF-κB and NLRP3 Signal Pathway

Panax ginseng as a potential therapeutic for neurological disorders associated with COVID-19; Toward targeting inflammasome

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