Using the Resonance Chiral Theory Lagrangian, we perform a calculation of the vector form factor of the pion at the next-to-leading order (NLO) in the 1/N C expansion. Imposing the correct QCD short-distance constraints, one fixes the amplitude in terms of the pion decay constant F and resonance masses. Its low momentum expansion determines then the corresponding O(p 4 ) and O(p 6 ) low-energy chiral couplings at NLO, keeping control of their renormalization scale dependence. At µ 0 = 0.77 GeV, we obtain L 9 (µ 0 ) = (7.9 ± 0.4) · 10 −3 and C 88 (µ 0 ) − C 90 (µ 0 ) = (−4.6 ± 0.4) · 10 −5 .