Renoprotective Effects of Gallic Acid Against Gentamicin Nephrotoxicity Through Amelioration of Oxidative Stress in Rats

Abstract

Gallic acid (GA), as a strong antioxidant, was selected in this study to investigate its possible nephroprotective effects against gentamicin (GM)-induced nephrotoxicity. Twenty-four rats were separated into three groups (n=8): group 1 (control group) received saline (0.5 mL/day), group 2 (GM group) received GM (100 mg/kg/day), and group 3 (treated group) received GM (100 mg/kg/day) and GA (100mg/kg/day). All treatments were performed intraperitoneally for 12 days. After 12 days, the rats were euthanized, and kidneys were removed immediately. For serum preparation, blood samples were collected before killing. Kidney paraffin sections were prepared from one of the kidneys and stained by the periodic acid-Schiff process. GA significantly decreased GM-induced renal histopathological injuries, including tubular necrosis, tubular cast, and leucocyte infiltration compared with the GM group. Additionally, GA significantly improved proteinuria, serum levels of urea and creatinine, and serum activities of aspartate aminotransferase (AST) and alanine HIGHLIGHTS  Gallic acid ameliorates gentamicin-induced nephrotoxicity.  Gallic acid decreases levels of NO and MDA and enhances abilities of GSH, GPX, and CAT.  Gallic acid improves altered liver and renal function markers in nephrotoxic animals.  Gallic acid reduces renal histopathological injuries.  2 Ahmadvand, H.; et al.

Comparison of the therapeutic role of garlic extract and verapamil against gentamicin-induced nephrotoxicity in rats

“…The results of the current study showed a significant increase in the concentration of malondialdehyde, in contrast to the significant decrease in the concentration of reduced glutathione and catalase in the group treated with gentamycin compared to the control group and other groups, and the results were in agreement with what was confirmed by many studies(23)(24) (25).…”

“…The kidneys are the most affected and damaged organ due to the increased production of reactive oxygen species, which consist of large amounts of long chains of unsaturated fatty acids in the lipid component of the kidneys. The aminoglycoside group is a nephrotoxic antibiotic that causes nephrotoxicity due to increased production of free radicals and decreased antioxidant activity in them(24) (25).…”

Protective Effect of Gallic Acid on Cyclophosphamide-Induced Nephrotoxicity, Oxidative Stress, Genotoxicity, and Histopathological Alterations in Male Albino Rats

“…On the other hand, our study revealed the anti-inflammatory and antioxidant efficacy of GA in a dose-dependent manner. It plays a nephroprotective role by reserving and stabilizing the cell membranes integrity and renal architecture via scavenging activities of free radical (Reckziegel et al, 2016;Ghaznavi et al, 2018;Ahmadvand et al, 2020& Nouri et al, 2021 Also, this study is strongly supported by the immunohistochemical expression of one of the most studied tight junction proteins, ZO-1. These proteins play an important role in maintaining the tight junction complex structure (Ahn et al, 2016) and signaling transduction (Murphy et al, 2013).…”

Coadministration of gallic acid abates zearalenone‐mediated defects in male rat's reproductive function

“…[61] Other studies have shown that GA, as an exogenous antioxidant, can augment GSH capability in the suppression of oxidative stress. [63] Our findings revealed increased RONS levels in rats treated with ZEN alone in the hypothalamus, testis, and epididymis, which may be injurious to reproductive health. [64] Conversely, GA-abated RONS levels in the cotreated rat cohorts more effectively at the higher dose (GA: 40 mg/kg).…”