Renoprotective effect of Tanshinone IIA against kidney injury induced by ischemia-reperfusion in obese rats

Abstract

Objective: Obesity enhances the frequency and severity of acute kidney injury (AKI) induced by renal ischemiareperfusion (IR). Tanshinone IIA (TIIA) pre-treatment was used to alleviate renal injury induced by renal IR, and whether TIIA can attenuate renal cell apoptosis via modulating mitochondrial function through PI3K/Akt/Bad pathway in obese rats was examined. Methods: Male rates were fed a high-fat diet for 8 weeks to generate obesity, followed by 30 min of kidney ischemia and 24 h reperfusion induced AKI. The male obese rates were given TIIA ( 5mg/kg.d, 10 mg/kg.d, and 20 mg/kg.d) for 2 weeks before renal IR. Results: TIIA alleviated the pathohistological injury and apoptosis induced by IR. In addition, TIIA improved renal function, inflammatory factor, and balance of oxidation and antioxidation in obese rats after renal IR. At the same time, TIIA can inhibit cell apoptosis by improving mitochondrial function through the PI3K/Akt/Bad pathway. Mitochondrial dysfunction was supported by decreasing intracellular ATP, respiration controlling rate (RCR), mitochondrial membrane potential (MMP), and mitochondrial respiratory chain complex enzymes, and by increasing ROS, the opening of mitochondrial permeability transition pore (mPTP), and the mtDNA damage. The injury to mitochondrial dynamic function was assessed by decreasing Drp1, and increasing Mfn1/2; and the injury of mitochondrial biogenesis was assessed by decreasing PGC-1, Nrf1, and TFam.

Diallyl trisulfide improves spinal cord ischemia–reperfusion injury damage by activating AMPK to stabilize mitochondrial function

“…Moreover, mitochondria are dynamic organelles, which are in a dynamic balance of constant fission and fusion. Governed by related proteins containing Mfn2 and Drp1, mitochondria maintain a relatively stable size and morphology [ 24 ]. Our study found that after SCII surgery, mitochondrial morphology changed with obvious swelling and disappearance of cristae.…”

Renoprotective Effects of Tanshinone IIA: A Literature Review

“…Yang et al [ 55 ] found that Tan IIA may have a protective effect on the hypoxia/reperfusion (H/R) of HK-2 cells by reducing apoptosis through the inhibition of the oxidative stress response. Tai et al [ 56 ] found that Tan ⅡA pretreatment could regulate mitochondrial function through the PI3K/Akt/Bad pathway in obese rats, thereby reducing RIRI-induced apoptosis, inflammatory factors, oxidation and the antioxidant balance, improving renal function and reducing renal pathological damage in obese rats.…”