Renoprotective effect of Nigella sativa against cisplatin-induced nephrotoxicity and oxidative stress in rat

Hosseinian, Sara; Hadjzadeh, Mousa‐Al‐Reza; Roshan, Nema Mohamadian; Khazaei, Majid; Shahraki, Samira; Mohebbati, Reza; Rad, Abolfazl Khajavi

doi:10.4103/1319-2442.225208

Cited by 18 publications

(4 citation statements)

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“…Changes in serum creatinine concentration more reliably reflect changes in Glomerular filtration rate (GFR) than do changes in serum urea concentrations (34). The rise in serum level of these chemicals indicates a decline or failure in renal function to filter waste products from the blood and excrete them in the urine (35). In the present study, the sub-acute oral administration of the extracts did not show significant alteration in the serum levels of urea and creatinine concentrations in Mice administered with both doses as compared to the controls.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening, Investigation of the toxic and hepatoprotective effect of leaves ofPrunus africana-using mice model

Dabe,

Kefale,

Addo

et al. 2024

Preprint

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Purpose: Liver disease is a growing public health problem both in developing and developed countries. Allopathic medicines available for liver disease are not accessible and affordable for 3rd world countries, like Ethiopia. There is a need to search for alternative therapy, in which herbals are widely used in traditional medicine and promising for the development of effective and less costly treatment. As a result, this study aimed to screen phytochemicals and investigate the toxic and hepatoprotective effects of Prunus africana which is traditionally used in Benchi sheko and Sheka Zones, Southwest Ethiopia. Methods: Methanolic extracts of Prunus africana, were used to evaluate the toxicity and hepatoprotective activity in both male and female swiss Albino Mice. Liver injury was induced by carbon tetrachloride (CCl4). The toxicity and hepatoprotective activity were examined biochemically, histologically, and through the analysis of the general features of the study animals. The phytochemical composition was screened qualitatively using standard chemical tests for secondary metabolites. The results were expressed as Mean ± SD, and differences at P < 0.05 were considered significant. Differences between the experimental and control groups were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett's T-test to determine their level of significance. Results: The extract of Prunus africana revealed positive for the presence of flavonoids and Saponins but negative for Anthraquinone glycoside. The current study showed that the median oral lethal dose of the plant was greater than 5000mg/kg. The general behavior of the animal and organ weight, gross morphology, and the biochemical and histological parameters confirmed that the Methanolic leaves extract of this plant is safe during the sub-acute toxicity tests with a dose of 600 and 1800mg/kg. Methanolic extract before and after the CCl4 administration caused a significant reduction in the values of Alanine transaminase, Aspartate transaminase, Alkaline phosphatase, and bilirubin (P<0.05) almost comparable to the standard drug Silymarine. The hepatoprotective activity was supported by histopathological examination of the liver tissue of control and treated animals. Conclusion: The methanolic extract of Prunus africana at the test doses did not show significant toxicity. The results of this study also demonstrate that the extract was effective for the prevention of CCl4-induced hepatic damage in Mice. This study thus justifies that, the use of Prunus africana in the treatment of liver diseases and points out that this plant warrants further detailed investigation as a promising hepatoprotective agent.

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Section: Discussionmentioning

confidence: 99%

Phytochemical Screening, Investigation of the toxic and hepatoprotective effect of leaves ofPrunus africana-using mice model

Dabe,

Kefale,

Addo

et al. 2024

Preprint

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“…According to the experiments that were conducted on humans and animals, the main cause of nephrotoxicity induced by aminoglycosides is oxidative stress (20,21). Oxidative stress occurs when there is an imbalance between oxidants like ROS and antioxidants and is caused by a decrease in antioxidants or when they are depleted as a result of lipid peroxidation, inactivation of antioxidant enzymes, cellular running out of GSH, or destruction of DNA (22).…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Beta Carotene Against Amikacin Induced Nephrotoxicity

Khalaf¹,

Althanoon²

2023

Mil. Med. Sci. Lett.

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Background:The rapid loss of kidney function is known as nephrotoxicity. The harmful effects of medications may cause this. Amikacin-related nephrotoxicity is an excellent illustration of such a case. Amikacin is a synthetic aminoglycoside that works against the majority of gram-negative bacteria and, in some circumstances, can be harmful to the kidneys. One of the many methods used to prevent or decrease this toxicity is the use of antioxidant substances with amikacin. Beta carotene is an antioxidant carotenoid pigment. The aim of the current study: is to illustrate the ameliorative effect of beta carotene against the nephrotoxicity caused by amikacin when given before or during the treatment of amikacin. The study also evaluates the nephrotoxicity evoked by amikacin. Material and Methods: Five groups of animals were used (seven rats in each group): the control group, the amikacin group, the beta carotene group, the beta carotene with the amikacin group, and the group given beta carotene first, then added amikacin later together with the beta carotene. Results:The results of the current study demonstrate that when comparing the amikacin-treated group to the control group, there was a significant elevation (p ˂ 0.05) of serum creatinine and urea levels. The results also showed that when comparing the amikacin -treated group to the group treated with beta carotene during amikacin treatment for 14 days, there was a significant reduction (p ˂ 0.05) in serum creatinine and urea levels. Furthermore, in comparison of the amikacin -treated group to the group treated with beta carotene only for 9 days and continued with amikacin for 14 days, there was a significant reduction (p ˂ 0.05) in serum creatinine and urea levels. Also, there were significant decreases (near normal) in serum creatinine and urea. Histological findings confirmed these findings; there was damage to renal tissue in the amikacin -treated group, whereas groups treated with beta carotene and amikacin showed improvements in histological images. Discussion and conclusion: According to the results of renal function tests and histological findings, treatment with AMK only causes renal toxicity in rats, and when rats are treated with BC before and during AMK treatment or even only during AMK treatment, BC can prevent this renal toxicity by decreasing serum urea and creatinine levels, which, in agreement with the previous study, leads us to conclude that BC offers significant protection against AMK-induced nephrotoxicity, The results also showed the efficiency of two methods of administration of beta carotene in giving the required protection against renal toxicity (not preferring one method over the other).

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“…A recent study suggested that acute kidney injury is the most serious side effect of cisplatin, an important antitumor agent. Lipid peroxidation and oxygen free radicals are important mediators of cisplatin-induced nephrotoxicity [21]. The molecular role of TGF-1β is critical in driving mechanisms of cellular repair.…”

Section: Discussionmentioning

confidence: 99%

Coleus amboinicus extract increases transforming growth factor-1β expression in Wistar rats with cisplatin-induced nephropathy

Sahrial¹,

Solfaine²

2019

Vet World

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Background and Aim: Coleus amboinicus (CA) plants are known to exert antibacterial and anti-inflammatory effects and demonstrate antiproliferative effects against cancer cells. This study aimed to investigate the activity of CA extract on the expression of transforming growth factor-1β (TGF-1β) in cisplatin-induced nephropathy in Wistar rats (Rattus norvegicus). Materials and Methods: CA was obtained from fresh leaves of CA and was extracted using 96% ethanol maceration. This blinded, controlled, randomized post-test study assigned 24 Wistar rats to three groups (n=8). Negative controls received normal saline (P0), nephropathy was induced in rats by cisplatin (5 mg/kg, IP) (P1), and treated with ethanolic coleus extract (500 mg/kg, PO) (P2), respectively, for 7 days. Nephropathy was induced on the 4th day. All rats were sacrificed on the 8th day for blood and kidney sample collection. Concentrations of blood urea nitrogen (BUN), creatinine, and alkaline phosphatase were analyzed using colorimetric analysis. A semi-quantitative analysis was performed on sectioned kidneys to determine the numbers of positive cells for TGF-1β expression and to evaluate structural and functional alterations in the kidneys using histopathological and immunohistochemical staining. Results: The concentrations of BUN, creatinine, and alkaline phosphatase from blood samples in the treatment group were significantly lower than those of the control group (p<0.05). Morphological evaluation of the tubular interstitium and glomeruli revealed that necrotic, degenerating, and infiltration of cells significantly decreased in the treatment group compared to the control group (p<0.05). The mean immunostaining scores indicating the presence of TGF-1β were 7.8 in the ethanolic coleus extract group, 3 in the induction group, and 2.3 in the control group. The expression scores for TGF-β1 were significantly different between the ethanolic coleus extract treatment and control group (p<0.05). Conclusion: Our results suggest that in Wistar rats with cisplatin-induced nephropathy, CA extract inhibits pathological lesions by regulating the renal expression of TGF-1β in areas containing the renal tubules and glomeruli. Keywords: cisplatin, Coleus amboinicus, nephropathy, transforming growth factor-1β.

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Renoprotective effect of Nigella sativa against cisplatin-induced nephrotoxicity and oxidative stress in rat

Cited by 18 publications

References 0 publications

Phytochemical Screening, Investigation of the toxic and hepatoprotective effect of leaves ofPrunus africana-using mice model

Phytochemical Screening, Investigation of the toxic and hepatoprotective effect of leaves ofPrunus africana-using mice model

The Protective Effect of Beta Carotene Against Amikacin Induced Nephrotoxicity

Coleus amboinicus extract increases transforming growth factor-1β expression in Wistar rats with cisplatin-induced nephropathy

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