Renoprotective effect of edaravone in acute limb ischemia/reperfusion injury

İnce, İlker; Akar, İlker; Arıcı, Akgül

doi:10.5606/tgkdc.dergisi.2020.18905

Cited by 7 publications

(6 citation statements)

References 27 publications

(33 reference statements)

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“…The reactive oxygen species (ROS), which can cause direct damage to lipids in cell membranes and also to the intracellular structures such as nucleic acids and mitochondria, are the major mediators of these harmful effects. [ 1 ] The ROS can induce the production of proinflammatory cytokines that trigger acute tissue inflammation and injury in the ischemic and remote organs through activating various molecules, including the intracellular signaling molecule nuclear factor kappa beta (NF-kB). [ 2 ]…”

Section: Introductionmentioning

confidence: 99%

Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

Kurtoğlu¹,

Durmaz²,

Rahman³

et al. 2021

Turk Gogus Kalp Dama

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Background: This study aims to investigate the effects of 2-aminoethoxydiphenyl borate (2-APB) on aortic clamping-induced lung and kidney tissue oxidation, tissue inflammation, and histological damage in a rat model. Methods: A total of 28 adult female Wistar albino rats were randomly allocated to four equal groups: Control group, ischemia-reperfusion group, dimethyl sulfoxide group, and 2-APB group. Animals in the control group underwent median laparotomy. In the remaining groups, supra-celiac aorta was clamped for 45 min and, then, reperfusion was constituted for 60 min. The 2-APB (2 mg/kg) was administered before clamping. The remaining groups received saline (ischemia-reperfusion group) or dimethyl sulfoxide (dimethyl sulfoxide group). Kidney and lung tissue samples were harvested at the end of reperfusion. Results: Aortic occlusion caused increased tissue total oxidant status and reduced total antioxidant status and glutathione levels in the ischemia-reperfusion and dimethyl sulfoxide groups. Tissue interleukin-1 beta and tumor necrosis factor-alpha levels, nuclear factor kappa beta activation, and histological damage severity scores were also higher in these groups. The 2-APB treatment eliminated the increase in total oxidant status and the decrease in total antioxidant status and glutathione levels. It also caused a decrease in the interleukin-1 beta levels, although it did not significantly alter the tumor necrosis factor-alpha levels, nuclear factor kappa beta immunoreactivity, and histological damage scores. Conclusion: Borate exerted a beneficial antioxidant effect as evidenced by reduced oxidative stress; however, it did not inhibit nuclear factor kappa beta activation and prevent histological damage in supra-celiac aortic clamping-induced kidney and lung injury in rats.

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Section: Introductionmentioning

confidence: 99%

Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

Kurtoğlu¹,

Durmaz²,

Rahman³

et al. 2021

Turk Gogus Kalp Dama

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“…In practical terms, there would be no reason to deploy tourniquets in the absence of active hemorrhage. However, conventional murine models in the literature were produced with application of tourniquets without hemorrhage (Klausner et al, 1989;Adachi et al, 2006;Hsu et al, 2012;Yang et al, 2012;Mansour et al, 2014;Kao et al, 2015a;Kao et al, 2015b;Karahan et al, 2016;Onody et al, 2016;Foster et al, 2017;Shih et al, 2018;İnce et al, 2019;Packialakshmi et al, 2022). We describe a new TILLIR injury model, which was produced by first inducing hemorrhage then applying tourniquets.…”

Section: Discussionmentioning

confidence: 98%

“…Although pigs are more similar to humans in physical size, physiology and immunology than rodents (Packialakshmi et al, 2020;Zhou, 2022), rodents are easy to handle, quickly reproduce and have contributed significantly to deciphering pathophysiology and developing novel therapies. As such, a majority of tourniquet-induced lower limb ischemia/ reperfusion (TILLIR) injury in the literature has been produced in rodents, and a variety of therapies have been tested using rodent models (Klausner et al, 1989;Adachi et al, 2006;Hsu et al, 2012;Mansour et al, 2014;Kao et al, 2015a;Kao et al, 2015b;Karahan et al, 2016;Onody et al, 2016;Foster et al, 2017;Shih et al, 2018;İnce et al, 2019).…”

Section: Figurementioning

confidence: 99%

A clinically-relevant mouse model that displays hemorrhage exacerbates tourniquet-induced acute kidney injury

Packialakshmi,

Burmeister,

Anderson

et al. 2023

Front. Physiol.

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Hemorrhage is a leading cause of death in trauma. Tourniquets are effective at controlling extremity hemorrhage and have saved lives. However, tourniquets can cause ischemia reperfusion injury of limbs, leading to systemic inflammation and other adverse effects, which results in secondary damage to the kidney, lung, and liver. A clinically relevant animal model is critical to understanding the pathophysiology of this process and developing therapeutic interventions. Despite the importance of animal models, tourniquet-induced lower limb ischemia/reperfusion (TILLIR) models to date lack a hemorrhage component. We sought to develop a new TILLIR model that included hemorrhage and analyze the subsequent impact on kidney, lung and liver injuries. Four groups of mice were examined: group 1) control, group 2) hemorrhage, group 3) tourniquet application, and group 4) hemorrhage and tourniquet application. The hemorrhagic injury consisted of the removal of 15% of blood volume through the submandibular vein. The tourniquet injury consisted of orthodontic rubber bands applied to the inguinal area bilaterally for 80 min. Mice were then placed in metabolic cages individually for 22 h to collect urine. Hemorrhage alone did not significantly affect transcutaneous glomerular filtration rate (tGFR), blood urea nitrogen (BUN) or urinary kidney injury molecule-1 (KIM-1) levels. Without hemorrhage, TILLIR decreased tGFR by 46%, increased BUN by 162%, and increased KIM-1 by 27% (p < 0.05 for all). With hemorrhage, TILLIR decreased the tGFR by 72%, increased BUN by 395%, and increased urinary KIM-1 by 37% (p < 0.05 for all). These differences were statistically significant (p < 0.05). While hemorrhage had no significant effect on TILLIR-induced renal tubular degeneration and necrosis, it significantly increased TILLIR-induced lung total injury scores and congestion, and fatty liver. In conclusion, hemorrhage exacerbates TILLIR-induced acute kidney injury and structural damage in the lung and liver.

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“…According to the MRI results before and after treatment, the tissue bleeding caused by ischemia-reperfusion injury in the two groups has improved, but the observation group is significantly better than the control group. A large number of studies have shown that edaravone can directly scavenge hydroxyl radicals through electrons and inhibit the chain reaction of cell membrane peroxidation, thereby effectively reducing the damage of intracellular oxygen free radicals [5,17], to indirectly alleviate the bleeding caused by vascular endothelial cell injury. is study also analyzed the two groups of patients before and after treatment of serum SOD, MDA levels, and Bcl-2, Bax, and Caspase-3 protein levels.…”

Section: Discussionmentioning

confidence: 99%

“…Edaravone is a free radical scavenger, which is first used in the treatment of cerebral ischemia-reperfusion injury. The long-term clinical practice has proved that edaravone has a significant effect on the treatment of ischemic reperfusion injury [ 5 , 6 ]. Mailuoning, as a traditional Chinese medicine preparation with antithrombotic and blood viscosity reduction, has a protective effect on limb ischemia-reperfusion injury, but there is a lack of research on the therapeutic effect between it and edaravone.…”

Section: Introductionmentioning

confidence: 99%

Deep Learning Reconstruction Algorithm‐Based MRI Image Evaluation of Edaravone in the Treatment of Lower Limb Ischemia‐Reperfusion Injury

Liu

Duan

et al. 2022

Contrast Media & Molecular Imaging

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This research aimed to evaluate the therapeutic effect of edaravone on lower limb ischemia-reperfusion injury by MRI images of graph patch-based directional curvelet transform (GPBDCT), compression reconstruction algorithm. 200 patients with lower limb ischemia-reperfusion injury after replantation of severed limb were randomly divided into the observation group (edaravone treatment) and control group (Mailuoning injection treatment), with 100 cases in each group. MRI scanning and image processing using the GPBDCT algorithm were used to evaluate the therapeutic effect of the two groups of patients. The results showed that the signal noise ratio (SNR) (22.01), relative l 2 norm error (RLNE) (0.0792), and matching degree γ (0.9997) of the compression and reconstruction algorithm based on GPBDCT were superior to those of the conventional compression and reconstruction algorithm ( P < 0.05 ). MRI examination showed that the decrease of bleeding signal after treatment in the observation group was superior to that in the control group. The levels of superoxide dismutase (SOD) (15 ± 2.02), malondialdehyde (MDA) (2.27 ± 1.02), B cell lymphoma-2 (Bcl-2) (8.5 ± 1.02), Bcl-2-associated X (Bax) (3.7 ± 0.42), and Caspase-3 protein (35.9 ± 5.42) in the observation group before and after treatment were significantly higher than those in the control group ( P < 0.05 ). In conclusion, the GPBDCT-based compression reconstruction algorithm has a better effect on MRI image processing, and edaravone can better remove free radicals and alleviate apoptosis.

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Renoprotective effect of edaravone in acute limb ischemia/reperfusion injury

Cited by 7 publications

References 27 publications

Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

A clinically-relevant mouse model that displays hemorrhage exacerbates tourniquet-induced acute kidney injury

Deep Learning Reconstruction Algorithm‐Based MRI Image Evaluation of Edaravone in the Treatment of Lower Limb Ischemia‐Reperfusion Injury

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