Renoprotective effect of aliskiren monotherapy and aliskiren−pentoxifylline combination in hypertensive-diabetic type 2 patients with diabetic nephropathy

Hamed, Amin T.; Taha, Mohammed; Nasser, Luay M.

doi:10.1016/j.bfopcu.2013.06.002

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…DN is a serious complication of diabetes mellitus, which not only increases the mortality of diabetic patients, but also affects the patients' life quality [35]. Recently, the use of Chinese herbs and their extracts in the treatment of DN has achieved good results [36].…”

Section: Discussionmentioning

confidence: 99%

Arbutin protects HK-2 cells against high glucose-induced apoptosis and autophagy by up-regulating microRNA-27a

Zhang

Tian³

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Arbutin (ARB) has been widely used in skin pigmentation disorders. Nevertheless, the involvements of ARB in diabetic nephropathy (DN) are still unknown. We investigated the functions of ARB in high glucose (HG)-induced cell apoptosis and autophagy in HK-2 cells. Cell viability was examined through CCK-8 in HK-2 cells after disposal with 45 mM glucose and ARB (10-50 lM). Flow cytometry and western blot tested cell apoptosis and the related protein levels in HK-2 cells after 45 mM glucose and 50 lM ARB administration. RT-qPCR delved microRNA (miR)-27a expression in HG and ARB co-treated HK-2 cells. Effect of miR-27a on ARB affected cell apoptosis and autophagy was investigated after miR-27a inhibitor transfection. JNK and mTOR pathways were finally assessed by western blot. ARB alleviated HG-induced cell apoptosis, autophagy and regulated the related protein levels in HK-2 cells. MiR-27a expression was reduced in HG-treated cells, but was accelerated in HG and ARB co-treated HK-2 cells with the increased concentration. Inhibition of miR-27a apparently abolished the outcomes of ARB in HG-induced HK-2 cells apoptosis and autophagy. Besides, ARB blocked JNK and mTOR pathways by regulating miR-27a. The findings demonstrated that ARB alleviated apoptosis and autophagy in HGtreated HK-2 cells by regulating miR-27a/JNK/mTOR axis.

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Section: Discussionmentioning

confidence: 99%

Arbutin protects HK-2 cells against high glucose-induced apoptosis and autophagy by up-regulating microRNA-27a

Zhang

Tian³

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…The results reveal also that the aliskirenpentoxifylline combination decreased the levels of serum urea and creatinine significantly, our results are in agreement with the results obtained by A.T. Hamed et al [34], who investigated the renoprotective effect of aliskiren alone and in combination with pentoxifylline, in type 2-diabetic hypertensive patients with diabetic nephropathy, and have shown that an aliskiren-pentoxifylline combination (150, and 400 mg/day, respectively) significantly reduced urinary albumin excretion (UAE) rate after 6 and 9 months of treatment, and also reduced serum creatinine level throughout the study period.…”

Section: Discussionsupporting

confidence: 93%

The Renal and Cardio Protective Effects of Aliskiren and Pentoxifylline Alone and in Combination on Streptozotocin Induced Diabetic Rats

El-Mahdy

El‐Masry

El-Desouky

et al. 2016

BJPR

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Background/Aims: The aim of the present study is to investigate the renoprotective and cardioprotective effect of aliskiren and pentoxifylline, alone and in combination, on streptozotocin (STZ) induced diabetic rats. Methods: Fifty male Sprague Dawley rats were divided into 5 groups of 10 rats each, namely the control (Group I), diabetes (Group II), diabetic treated with aliskiren (Group III), diabetic treated with pentoxifylline (Group IV), and diabetic treated with aliskiren-pentoxifylline combination (Group V). Aliskiren (10 mg/kg/day) and pentoxifylline (55 mg/kg/ day) were given by oral-gavage once daily for 8 weeks. Renal function, oxidative stress parameters, immunohistochemical detection for transforming growth factor β, and kidney and heart histology were determined.

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Anti-diabetic and renoprotective effects of aliskiren in streptozotocin-induced diabetic nephropathy in female rats

Mahfoz

El-Latif

Ahmed

et al. 2016

Naunyn-Schmiedeberg's Arch Pharmacol

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Since chronic kidney disease due to diabetic nephropathy (DN) is becoming an ever larger health burden worldwide, more effective therapies are desperately needed. In the present study, the anti-diabetic and renoprotective effects of aliskiren have been evaluated in streptozotocin (STZ)-induced DN in rats. DN was induced by a single intraperitoneal injection of STZ (65 mg/kg). Three weeks after STZ, rats were divided into four groups; normal, diabetic, diabetic treated with gliclazide (10 mg/kg/day) for 1 month, and diabetic treated with aliskiren (50 mg/kg/day) for 1 month. At the end of the experiment, mean arterial blood pressure and heart rate were recorded. Rats were then euthanized and serum was separated for determination of glucose, insulin, kidney function tests, superoxide dismutase activity (SOD), adiponectin, and tumor necrosis factor-alpha (TNF-α). One kidney was used for estimation of malondialdehyde (MDA), reduced glutathione (GSH), and nitric oxide (NO) contents. Other kidney was used for histopathological study and immunohistochemical measurement of caspase-3 and transforming growth factor beta (TGF-β). In addition, islets of Langerhans were isolated from normal rats by collagenase digestion technique for in vitro study. Aliskiren normalized STZ-induced hyperglycemia, increased insulin level both in vivo and in vitro, normalized kidney function tests and blood pressure, and alleviated STZ-induced kidney histopathological changes. This could be related to the ability of aliskiren toward preserving hemodynamic changes and alleviating oxidative stress and inflammatory and apoptotic markers induced by STZ in rats. However, aliskiren was more effective than gliclazide in relieving STZ-induced DN. These findings support the beneficial effect of aliskiren treatment in DN which could be attributed to its anti-diabetic, renoprotective, antioxidant, anti-inflammatory, and anti-apoptotic effects. Moreover, clinical studies are required to establish the effectiveness of aliskiren treatment in patients suffering from hypertension and diabetes.

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Renoprotective effect of aliskiren monotherapy and aliskiren−pentoxifylline combination in hypertensive-diabetic type 2 patients with diabetic nephropathy

Cited by 4 publications

References 32 publications

Arbutin protects HK-2 cells against high glucose-induced apoptosis and autophagy by up-regulating microRNA-27a

Arbutin protects HK-2 cells against high glucose-induced apoptosis and autophagy by up-regulating microRNA-27a

The Renal and Cardio Protective Effects of Aliskiren and Pentoxifylline Alone and in Combination on Streptozotocin Induced Diabetic Rats

Anti-diabetic and renoprotective effects of aliskiren in streptozotocin-induced diabetic nephropathy in female rats

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