Rennet-Induced Milk Coagulation by Continuous Steady Shear Stress

Konuklar, Gül; Gunasekaran, Sundaram

doi:10.1006/jcis.2002.8321

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…Shear stresses similar to the one tested in this work can be found in different industrial equipment, pipes and accessories (Ahmed et al, 2011;Konuklar and Gunasekaran, 2002;Lelièvre et al, 2002;Liu et al, 2006).…”

Section: Cell Adhesion and Biofilm Assayssupporting

confidence: 75%

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Moreira

Gomes

Simões

et al. 2015

Food and Bioproducts Processing

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Abstract:In the food industry, biofilm formation in pipes, equipment and cooling systems increases maintenance costs, decreases operational efficiencies and is a source of contamination.Shear stress, nutrient load and surface material are important variables affecting the biofilm onset in industry. In this work, the combined impacts of these variables were assessed using three different materials (glass, copper and stainless steel), two nutrient loads (high and low nutrient medium) and two hydrodynamic conditions (static and dynamic). Initial adhesion and biofilm formation were studied in microplates usingEscherichia coli as a model organism.Surface material was the factor with the strongest impact and adhesion/biofilm formation were correlated with surface hydrophobicity. However, the impact of this variable was dependent on the nutrient load and imposed shear stress. It was also found that, for the majority of the situations tested, initial attachment performance is a good predictor of biofilm formation behaviour and that the effects observed during attachment are amplified during biofilm maturation. Since shear stress is a major determinant in cell adhesion, the results of this study may find application in industrial systems operating at flow rates between 0.001 and 600 m 3 h -1 depending on tube material and diameter.

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Section: Cell Adhesion and Biofilm Assayssupporting

confidence: 75%

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Moreira

Gomes

Simões

et al. 2015

Food and Bioproducts Processing

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“…6a), demonstrated viscosity values starting with 0.55 Pa.s at a minimum stress of 0.05 Pa, till rather thinner cultures (0.32 Pa.s) were obtained at a maximum of stress of 0.4 Pa. Tannins (gallic acid derivatives) were found to induce extracellular polysaccharide (EPS) production in S. gallolyticus and S. mutans (Krause et al 2005), which could be responsible for the higher apparent viscosity of milk cultures prepared with gallic acid adapted cells. Their decreased viscosity at increasing stress rates (Kristensen et al 1997) may be elicited from the relaxation of bonds between cross-linkages of protein strands of casein fused with the slime polysaccharide molecules produced (Konuklar and Gunasekaran 2002). On the other hand, milk cultures inoculated with catechin adapted cells (Fig.…”

Section: Cell Surface Hydrophobicitymentioning

confidence: 98%

Influence of gallic acid and catechin polyphenols on probiotic properties of Streptococcus thermophilus CHCC 3534 strain

Khalil

2010

World J Microbiol Biotechnol

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The study aimed at assessing the potential influences of two polyphenolic compounds on growth and some recently proven probiotic attributes of Streptococcus thermophilus CHCC 3534. The strain was tolerant to 0.8% gallic acid and 0.3% catechin. Gallic acid and catechin adapted cells were moderately to highly sensitive to most antibiotics, and to low pH (2.0) compared to the parental cells. Gallic acid adapted cells survived pHs 3.0 and 4.0, and were rapid b-galactosidase producers. Catechin adapted cells demonstrated marked tolerance to 0.4% oxgall, and possessed low hydrophobicity to organic solvents tested. They also produced large capsules of 5.0 lm in diameter due to high content of glucose and galactose monomers as determined by gas chromatography mass spectroscopy. Matrix-assisted laser-desorption time-offlight mass spectrometry analysis detected small peptide subunits of masses 656 Da in bacteriocins of adapted cells that significantly inhibited the growth of Escherichia coli O157:H7, Pseudomonas aeroginosa, and Listeria monocytogenes. The data demonstrates that selection of S.thermophilus 3534 strain in dairy products and formulations supplemented with polyphenolics may present a nutritional benefit as a promising probiotic starter candidate as well as a potential source of dietary antioxidants.

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“…As an endopeptidase, pepsin decomposes most proteins and peptides to oligopeptides, and has milk-curdling activity (Konkular and Gunasekaram, 2002). Moreover, this enzyme is capable of efficient proteolysis, even at low temperatures (Ahn et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Its potential applications in food production or biomedical industries have become more common and have replaced many traditional methods. For example, bovine or porcine pepsin is regarded as a substitute for calf rennet or is used for protein digestion prior to studies on identification of proteins as well as characterization and production of bioactive peptides (Konkular and Gunasekaram, 2002;Ticu et al, 2004;Shukla and Devi, 2005). Especially in the latter practical application of pepsin, immobilization is a prerequisite step that allows increasing the stability of biocatalysts in order to achieve higher profitability (Cooper et al, 1990, Kamatari et al, 2003Gamze and Senay, 2007).…”

Section: Introductionmentioning

confidence: 99%

Novel Application of Porous and Cellular Materials for Covalent Immobilization of Pepsin

Szałapata

Osińska-Jaroszuk

Bryjak

et al. 2016

Braz. J. Chem. Eng.

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-Pepsin was immobilized via covalent bonds on different carriers: a silica gel carrier, acrylic beads, and a cellulose-based carrier -Granocel. All carriers were functionalized through the presence of -OH, -COOH, -NH 2 , or glycidyl groups on their surfaces. Three different cross-linkers were used for activation thereof. The results showed that Granocel activated by glutaraldehyde or carbodiimide and silica gel activated by glutaraldehyde were suitable carriers for the expression of enzyme activity. The optimum pH range for the native enzyme was 2.5-3.5 and this range was extended to the value 6.5 in the case of enzyme immobilized on the silica gel carrier and on Granocel. The optimum temperature values for the native and immobilized enzyme were in the range 37-40 °C and 40-50 °C, respectively. The activity of the immobilized pepsin at different values of pH and temperature was higher in comparison with the activity of the free enzyme.

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Rennet-Induced Milk Coagulation by Continuous Steady Shear Stress

Cited by 7 publications

References 22 publications

The impact of material properties, nutrient load and shear stress on biofouling in food industries

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Influence of gallic acid and catechin polyphenols on probiotic properties of Streptococcus thermophilus CHCC 3534 strain

Novel Application of Porous and Cellular Materials for Covalent Immobilization of Pepsin

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