Renin Exists in Human Adrenal Tissue*

Naruse, Mitsuhide; Sussman, Craig R.; Naruse, Kiyoko; Jackson, Richard V.; Inagami, Tadashi

doi:10.1210/jcem-57-3-482

Cited by 78 publications

(40 citation statements)

References 27 publications

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“…Renin has been shown, immunohistochemically, to exist in human adrenal tissue, and biochemical characterization of this adrenal enzyme has demonstrated similarities between this renin and purified kidney renin [11]. The renin activity was predominantly in the cortex rather than in the medulla of the adrenal tissue.…”

Section: Discussionmentioning

confidence: 95%

A Case of Renin-Producing Adrenocortical Cancer.

et al. 2000

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Abstract.Here we report a case of a renin-producing adrenocortical carcinoma. A 57-year-old woman was referred to our hospital complaining of thirst and generalized muscle weakness. She was diagnosed as being hypertensive and diabetic with associated hypokalemia and she had a hard elastic mass with a diameter of 10 cm on the left side of her neck. An abdominal computed tomography scan revealed a suprarenal mass on the left side (8.5 x 8 x 6.5 cm). Endocrinological examination demonstrated a marked elevation in the patient's serum glucocorticoid and sex steroid hormones as well as plasma renin activity. Histological examination of a sample taken from the neck mass revealed a metastasis from an adrenal carcinoma, which was stained positively with antibodies against cytochrome P450 and renin, establishing the diagnosis of a renin-producing adrenocortical carcinoma.Trilostane was effective in reducing serum cortisol levels, but mitotane was ineffective.

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Section: Discussionmentioning

confidence: 95%

A Case of Renin-Producing Adrenocortical Cancer.

et al. 2000

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“…Samples from differential and density gradient centrifugation were treated with 0.1 vol of phosphate buffer (0.2 M, pH 7.0) containing 1% Triton X-100 before assay. Specific immunoreactive renin activity was determined using antirenin antibody to distinguish it from nonspecific renin-like activity of proteases according to the method previously detected (4,5,15,16). 25 to 50 Ml of the samples were incubated with 10 ,ul ofdiluted (1:500) rabbit antiserum raised against pure rat kidney renin (17) at 4VC for 20 h. This antibody used resulted in 50% inhibition of renin activity (40.0 ng Ang I/h) in a rat kidney extract at a dilution of 1:10,000 and complete inhibition at a dilution of 1:1,000.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, renin plays a central role in blood pressure regulation. On the other hand, recent biochemical and immunological studies revealed that renin exists in the adrenal gland of various mammalian species (1)(2)(3)(4)(5). It was also demonstrated that this adrenal renin is primarily located in the zona glomerulosa cells rather than the fasciculata or medullary cells (6,7).…”

Section: Introductionmentioning

confidence: 99%

“…Also unknown is whether the renin is secreted from the adrenal. This intriguing question is raised by the findings ofthe presence of renin-like activity and ofimmunoreactive Ang I and Ang II in the plasma of anephric subjects (1 [1][2][3][4][5][6][7][8][9][10][11][12][13], and the hypotensive response to converting enzyme inhibitor, captopril, by a sodium-depleted anephric subject (14), suggesting that renin or renin-like enzymes synthesized in extrarenal tissues may contribute to the generation of plasma angiotensin. In view of these interesting issues, we tested the hypothesis that there exist renin-secretory granules in the adrenal.…”

Section: Introductionmentioning

confidence: 99%

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Presence of renin secretory granules in rat adrenal gland and stimulation of renin secretion by angiotensin II but not by adrenocorticotropin.

Mizuno¹,

Hoffman²,

McKenzie³

et al. 1988

J. Clin. Invest.

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Renin has been identified biochemically and immunohistochemically in the adrenal gland. We examined the subcellular distribution and behavior of adrenal renin. By differential centrifugation of adrenal capsules, we found renin mainly in mitochondrial fractions. By Percoll density gradient centrifugation of this fraction, dense granules were separated from mitochondria and microsomes. The renin activity in the dense granules from the capsules of nephrectomized rats was 15 times greater than that of the intact rat. Immunohistochemical studies revealed that the dense granules increased in number after bilateral nephrectomy. Immunogold staining of these granules showed unequivocally the presence of renin in these granules. Adrenal capsules in organ culture were found to release renin at a steady rate. Renin release from bilaterally nephrectomized rat adrenals was 46 times faster than from the organs of intact animals. The mechanism of the control of renin secretion from the adrenal gland was different from the kidney in that the secretion was stimulated by potassium chloride (10 mM) or angiotensin II (10--10-M) but not by ACI'H (10-9-10-7 M), suggesting stimulation by intracellular calcium. These results provide evidence that the adrenal synthesizes renin, stores it in specific secretory granules and secretes it in a regulated manner.

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“…Renin-like activity has been detected in the adrenal glands of rat (Ryan 1967, Ganten et al 1974, Naruse & Inagami 1982, Doi et al 1983, 1984a, Inagami et al 1984, Nakamaru et al 1985 and other species (Hayduk et al 1970, Ganten et al 1976, Naruse et al 1983, 1984, Racz et al 1992. Nephrectomy elicits a rapid and marked increase in the adrenal renin activity indicating that this enzyme is endogenous to the adrenal rather than due to contamination by plasma renin of renal origin (Naruse & Inagami 1982, Doi et al 1984a,b, Inagami et al 1984.…”

Section: Introductionmentioning

confidence: 98%

Transcription of (pro)renin mRNA in the rat adrenal cortex, and the effects of ACTH treatment and a low sodium diet

Ho¹,

Vinson²

1998

Journal of Endocrinology

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Transcription of the (pro)renin gene in the adult rat adrenal gland was studied by non-isotopic in situ hybridization.In glands from control (untreated) animals, transcription was relatively sparse, and occurred mostly in the outer zona fasciculata. Treatment with ACTH increased the apparent signal in both the glomerulosa and in fasciculata zones.A low sodium diet initially enhanced the transcription signal specifically in the glomerulosa, but as the regime was extended from 5 days to more than 2 weeks, the signal was also increased dramatically in the zona reticularis.The results emphasize the potential importance of the intraglandular renin-angiotensin system, particularly under conditions of chronic stimulation. They also suggest that angiotensin II, as well as being the major regulator of the glomerulosa, may also have some role in inner adrenocortical zone functions.

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Renin Exists in Human Adrenal Tissue*

Cited by 78 publications

References 27 publications

A Case of Renin-Producing Adrenocortical Cancer.

A Case of Renin-Producing Adrenocortical Cancer.

Presence of renin secretory granules in rat adrenal gland and stimulation of renin secretion by angiotensin II but not by adrenocorticotropin.

Transcription of (pro)renin mRNA in the rat adrenal cortex, and the effects of ACTH treatment and a low sodium diet

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