Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode

Liu, Songqin; Yu, Jiali; Ju, Huangxian

doi:10.1016/s0022-0728(02)01276-7

Cited by 156 publications

(56 citation statements)

References 34 publications

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“…Nilai K Mapp pada penelitian ini sebesar 6.04 mM dan I maks app sebesar 6.42 mA. Hasil yang diperoleh jauh lebih besar jika dibandingkan dengan hasil yang didapatkan oleh Liu et al (2003) yang mengimobilisasi tirosinase pada elektrode pasta karbon termodifikasi emas yang memeperoleh nilai KMapp sebesar 53 × 10 -3 mM dan nilai I maks app sebesar 12.3 × 10 -3 mA. Perbedaan ini dapat disebabkan oleh perbedaan jenis material penyangga yang digunakan sebagai matriks pengimobilisasi dan teknik pengimobilisasi yang digunakan.…”

Section: Gambar 6 Rentang Linier Antara L-dopa Dengan Tirosinaseunclassified

Ekstrak Metanol Rhizopora apiculata Sebagai Biosensor Penghambatan Aktivitas Tirosinase Menggunakan Elektrode Pasta Karbon Termodifikasi Ferosena Dan Zeolit

2018

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Ekstrak metanol tanaman Rhizopora apiculata telah dibuat untuk biosensor inhibitor enzim tirosinase menggunakan elektrode pasta karbon dengan termodifikasi zeolit dan mediator ferosena. Enzim tirosinase merupakan enzim yang mengkatalisis 2 tahap reaksi biosintesis melanin pada mamalia dan berperan dalam reaksi pencokelatan enzimatis. Enzim diimobilisasi pada permukaan transduser untuk menjaga aktivitas enzim. Aktivitas tirosinase diukur menggunakan substrat L-DOPA. Kondisi optimum aktivitas tirosinase berdasarkan pengoptimalan respon adalah tirosinase 50 U/mL dan zeolit 150 mg/mL dalam larutan penyangga fosfat. Penggunaan mediator ferosena dan material penyangga zeolit meningkatkan aktivitas tirosinase. Nilai KMapp dan Imaks app tirosinase ditentukan dengan metode Lineweaver-Burk. Nilai KM app dan Imaks app sebesar 6.04 mM dan sebesar 6.42 mM. Biosensor menggunakan zeolit sebagai matriks imobilisasi dan ferosensa sebagai mediator mempunyai stabilitas respon sampai 2 jam, dengan penurunan aktivitas sebesar 19%.

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Section: Gambar 6 Rentang Linier Antara L-dopa Dengan Tirosinaseunclassified

Ekstrak Metanol Rhizopora apiculata Sebagai Biosensor Penghambatan Aktivitas Tirosinase Menggunakan Elektrode Pasta Karbon Termodifikasi Ferosena Dan Zeolit

2018

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“…75 A tirosinase pode ser inibida por uma gama de poluentes, tais como carbamatos, organofosforados, ditiocarbamatos, atrazinas, tiuréas, ácidos carboxílicos aromáticos, triazinas, herbicidas, clorofenóis e agentes quelantes de cobre. [75][76][77][78][79][80][81][82][83][84][85][86] Desta forma, biossensores baseados em enzimas tirosinases podem ser empregados com biossensores baseados em inibição enzimática, detectando tanto o decrescimento da quantidade de oxigênio no meio reacional, quanto a formação da quinona, em presença do agente inibidor. Eletrodos de carbono, ouro, ouro modificado e eletrodos de Clark têm sido empregados na construção destes sensores.…”

Section: Biossensores Baseados Em Enzimas Tirosinasesunclassified

Biossensores baseados no processo de inibição enzimática

Marques¹,

Yamanaka²

2008

Quím. Nova

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Recebido em 3/5/07; aceito em 30/11/07; publicado na web em 4/9/08 BIOSENSORS BASED ON THE ENZYMATIC INHIBITION PROCESS. Fast, selective, reproducible and reliable detections have been carried out by using enzymatic biosensors in several areas. The enzymatic biosensors based on the inhibition represent an important role in analytical chemistry. Enzymes like cholinesterases, peroxidases, tyrosinases, etc. have been immobilized on electrochemical and optical transducers and the enzymatic activity decreasing in the presence of the inhibitor is related with its concentrations. This article presents a review on the enzymes used on the construction of these sensors, emphasizing the respective applications.

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“…The adsorption of colloidal gold nanoparticles on a chitosan membrane could provide a multilayer gold-nanoparticle assembly providing a suitable microenvironment, which is similar to the native environment of biomolecules. Based on this approach, a disposable biosensor with good detection precision and storage stability was fabricated for the rapid detection of H2O2 by entrapping horseradish peroxidase in a colloidal gold nanoparticle modified chitosan mem-brane [20,21]. An electrochemical biosensor can profit by combining gold nanoparticles with inorganic or organic nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Electroanalysis of urinary l-dopa using tyrosinase immobilized on gold nanoelectrode ensembles

Pinho

Subramanian²,

Ribeiro³

et al. 2012

J Appl Electrochem

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The performance of an amperometric biosensor constructed by associating tyrosinase (Tyr) enzyme with the advantages of a 3D gold nanoelectrode ensemble (GNEE) is evaluated in a flowinjection analysis (FIA) system for the analysis of Levodopa (Ldopa). GNEEs were fabricated by electroless deposition of the metal within the pores of polycarbonate track-etched membranes. A simple solvent etching procedure based on the solubility of polycarbonate membranes is adopted for the fabrication of the 3D GNEE. Afterwards, enzyme was immobilized onto preformed self-assembled monolayers of cysteamine on the 3D GNEEs (GNEE-Tyr) via cross-linking with glutaraldehyde. The experimental conditions of the FIA system, such as the detection potential (-0.200 V (vs. Ag/ AgCl)) and flow rates (1.0 mL min -1 ) were optimized. Analytical responses for L-dopa were obtained in a wide concentration range between 1 9 10 -8 mol L -1 and 1 9 10 -2 mol L -1 . The limit of quantification was found to be 1 9 10 -8 mol L -1 with a resultant % RSD of 7.23% (n = 5). The limit of detection was found to be 1 9 10 -9 mol L -1 (S/N = 3). The common interfering compounds namely, glucose (10 mmol L -1 ), ascorbic acid (10 mmol L -1 ), and urea (10 mmol L -1 ) were studied. The recovery of L-dopa (1 9 10 -7 mol L -1 ) from spiked urine samples was found to be 96%. Therefore, the developed method is adequate to be applied in the clinical analysis.

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Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode

Cited by 156 publications

References 34 publications

Ekstrak Metanol Rhizopora apiculata Sebagai Biosensor Penghambatan Aktivitas Tirosinase Menggunakan Elektrode Pasta Karbon Termodifikasi Ferosena Dan Zeolit

Ekstrak Metanol Rhizopora apiculata Sebagai Biosensor Penghambatan Aktivitas Tirosinase Menggunakan Elektrode Pasta Karbon Termodifikasi Ferosena Dan Zeolit

Biossensores baseados no processo de inibição enzimática

Electroanalysis of urinary l-dopa using tyrosinase immobilized on gold nanoelectrode ensembles

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