Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

Abstract

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins.

Two faces of Solanaceae telomeres: a comparison between <i>Nicotiana</i> and <i>Cestrum</i> telomeres and telomere-binding proteins

“…Therefore mutations that compromise the 3 overhang are likely to lead to telomere dysfunction (van Steensel et al, 1998). Previously we studied Asparagales species, including those with human-type telomeres (Rotkova et al, 2004Rotkova, 2007), and those of the Allium genus, where human-type telomeres and telomerase activity are absent Sykorova et al, 2006). The data revealed that these plants still express proteins which have specific binding to ancestral, Arabidopsis -type telomeric sequences.…”