Renal vasodilator activity of 5,6-epoxyeicosatrienoic acid depends upon conversion by cyclooxygenase and release of prostaglandins

Carroll, Mairéad A.; Balazy, Michael; Margiotta, Patricia; Falck, John R.; McGiff, John C.

doi:10.1016/s0021-9258(18)31383-8

Cited by 98 publications

(5 citation statements)

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“…Renal vascular resistance is decreased by adding 5,6-EET, 8,9-EET or 11,12-EET to the perfusate of iso-is not biphasic. The vasodilator effect of bradykinin on the Ef-Art via B 2 receptors is mediated by cP450 metabo-lated, perfused rabbit kidneys [28,29] traction [10,36,37]. The present study showed that the Af-Arts and found that it had a biphasic effect, inducing…”

Section: Effect Of No Synthesis Inhibition On Bradykinininduced Dilationmentioning

confidence: 54%

Mechanism involved in bradykinin-induced efferent arteriole dilation

Ren

Garvin

Carretero

2002

Kidney International

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Section: Effect Of No Synthesis Inhibition On Bradykinininduced Dilationmentioning

confidence: 54%

Mechanism involved in bradykinin-induced efferent arteriole dilation

Ren

Garvin

Carretero

2002

Kidney International

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“…These eicosanoids exhibit a range of biological activities affecting vascular and renal function and, therefore, contribute importantly to the regulation of fluid volume and blood pressure (1). Thus, they are vasodilator (2)(3)(4)(5)(6) or vasoconstrictor (7)(8)(9)(10), presumably through modulation of ion channels (11)(12)(13)(14); they also influence renal tubular function by modifying the activity of ion transport systems (15 -18). P450 AA products have been implicated in the autoregulation of renal blood flow (19,20), myogenic responses (21,22), and mediation/modulation of vasoactive hormones including angiotensin II (17,23), vasopressin (24), and bradykinin (25,26).…”

mentioning

confidence: 99%

A method for the determination of 5,6-EET using the lactone as an intermediate in the formation of the diol

Fulton

Falck

McGiff

et al. 1998

Journal of Lipid Research

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The 5,6 epoxyeicosatrienoic acid (5,6-EET) exhibits a range of biological activities but the functional significance of this labile eicosanoid is unknown due, in part, to difficulties of quantitation in biological samples. We have developed a sensitive and specific method to measure 5,6-EET utilizing its selective capacity to form a lactone. The initial conversion of 5,6-EET and 5,6-dihydr oxyeicosatrienoic acid (5,6-DHT) to 5,6-␦ -lactone is followed by selective purification using reverse phase high performance liquid chromatography (HPLC), reconversion to 5,6-DHT and quantitation by gas chromatography-mass spectrometry (GCMS). In oxygenated Krebs' buffer, 5,6-EET degrades to 5,6-␦ -lactone and 5,6-DHT with a t 1/2 Ϸ 8 min. In the presence of camphorsulfonic acid, 5,6-EET and 5,6-DHT convert to a single HPLC peak ( ‫؍‬ 205) comigrating with 5,6-␦ -lactone. Incubation of 5,6-␦ -lactone with triethylamine resulted in a single HPLC peak with the retention time of 5,6-DHT. In the perfusate from the isolated kidney, release of 5,6-EET (20 ؎ 5 pg/ml), measured indirectly via conversion to 5,6-DHT, was approx. 6-fold less than that reported for prostaglandin E 2 (PGE 2 ) and 20-HETE. The coronary perfusate concentration of 5,6 EET was 9 ؎ 2 pg/ml. 5,6-EET recovered from renal and coronary perfusates was increased 2-fold to 45.5 ؎ 5.5 pg/ml and 21.6 ؎ 6.3 pg/ml, respectively, by arachidonic acid.-

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“…Purity was assessed via thin layer chromatography and nuclear magnetic resonance (data not shown). 5,6-EET was not included due to its unique susceptibility to nonenzymatic or cyclooxygenase mediated cyclization (Carroll et al, 1993) and nonenzymatic conversion to the corresponding diol (DHET) (Jiang et al, 2004). The solutions of EETs and EETs mixtures and TPPU were prepared by adding them in DMSO and diluted in DMEM to give a final concentration of 1 µM, a level previously shown to reduce LPS-induced nitrite release in primary glial cells (Ghosh et al, 2020), with less than 0.1% DMSO at the final concentration in media.…”

Section: Rt-qpcrmentioning

confidence: 99%