Renal protein and ammonia biochemistry in NH4Cl acidosis and after uninephrectomy

Bignall, M.C.; Elebute, O; Lotspeich, WD

doi:10.1152/ajplegacy.1968.215.2.289

Cited by 24 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If uptake of orotic acid-8H into RNA can be equated with RNA synthesis, it can be said that there was decreased RNA synthesis up to 4 hr after the acid load. The fact that RNA levels and RNA: DNA ratio did not change is in keeping with the observation that in the rat kidney hypertrophied as a result of contralateral nephrectomy or acidosis, there is no change in renal RNA until after 24 and 36 hr (14). The depression of RNA synthesis in the presence of enhanced enzyme activity may indicate that there is a small separate fraction of RNA which codes for the PEPCK protein, is not affected by the general depression of RNA synthesis, and is undetectable since it is relatively small.…”

Section: Resultssupporting

confidence: 82%

“…The decrease in RNA synthesis shown by the decreased incorporation of orotic acid-2H into RNA is not reflected in the tissue RNA levels or the RNA: DNA ratios which remained normal throughout the 6 hr period of acidosis (Table IX). This ex- periment was not done at 48 hr, since it has already been reported ( 14) that at that time there is an increase of renal RNA.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Renal metabolic response to acid-base changes

Alleyne¹

1970

J. Clin. Invest.

View full text Add to dashboard Cite

A B STR A C T The early renal metabolic response was studied in rats made acidotic by oral feeding of ammonium chloride. 2 hr after feeding of ammonium chloride there was already significant acidosis. Urinary ammonia also increased after ammonium chloride ingestion and at 1 hr was significantly elevated. In vitro gluconeogenesis by renal cortical slices was increased at 2 hr and thereafter increased steadily. Ammonia production by the same slices was also increased at 2 hr, but thereafter fell and at 6 hr had decreased to levels which, although higher than those of the control, were lower than those obtained from the rats acidotic for only 2 hr. There was no correlation between in vitro gluconeogenesis and ammonia production by kidney slices from rats during the first 6 hr of acidosis, but after 48 hr of ammonium chloride feeding, these two processes were significantly correlated. The early increase in renal gluconeogenesis was demonstrable with both glutamine and succinate as substrates.The activity of the enzyme phosphoenolpyruvate carboxykinase was increased after 4-6 hr of acidosis. During this time there was a decrease in renal RNA synthesis as shown by decreased uptake of orotic acid-3H into RNA.Metabolic intermediates were also measured in quickfrozen kidneys at varying times after induction of acidosis. There was an immediate rise in aspartate and a fall in a-ketoglutarate and malate levels. There was never any difference in pyruvate or lactate levels or lactate: pyruvate ratios between control and acidotic rats. Phosphoenolpyruvate rose significantly after 6 hr of acidosis.All the data indicate that increased gluconeogenesis is an early response to metabolic acidosis and willThis work was presented in part at the 4th International

show abstract

Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Renal metabolic response to acid-base changes

Alleyne¹

1970

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Changes in activity of many other enzymes such as mitochondrial or those associated with gluconeogenesis or glycolysis are minimal or briefly depressed [48,227,263]. Some other changes in function during the first day or two are increased tissue/medium accumulation ratios of PAH and tetraethylammonium by cortex slices [15], tissue accumulation of isoaminobutyrate [427] and increased hexose phosphate shunt activity [155].…”

Section: Rapid Growth Period: 12 Hours To 2 Weeksmentioning

confidence: 99%

Compensatory Growth and Other Growth Responses of the Kidney

Wesson

1989

Nephron

View full text Add to dashboard Cite

“…14.5 ±0.39 (12) 91.4 ±3.12 (5) 3.2 ±0.11 (12) 797 ±49 (26) (19) 24.2 ±1.7 (29) 4.224±0.66 (13) 98.6 ±3.1 (26) 336 ±t9.9 (26) (7) 13.2 (7) 18.1 (7) 24.4 (7) 11.9…”

Section: Introductionmentioning

confidence: 99%

The effects of acidosis and alkalosis on the metabolism of glutamine and glutamate in renal cortex slices

Kamm¹,

Strope²

1972

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Studies of the metabolism of glutamine and glutamate by renal cortex slices from acidotic, alkalotic, and control rats were performed. 88-95% of the glutamine and 104-115% of the glutamate taken up from the medium could be accounted for by the products found. Acidosis increased glutamine uptake and conversion to ammonia, C02, glucose, lactate, pyruvate, lipid, and protein. The increase in glutamine conversion to ammonia after acidosis could be completely accounted for by the associated increase in its conversion to glucose, glutamate, lactate, and pyruvate. When glutamate metabolism was examined, acidosis did not affect substrate uptake but did increase its conversion to ammonia, glucose, lactate, C02, and lipid. The increase in 14CO2 from U-"C-glutamine and U-`4C-glutamate found with cortex slices from acidotic animals could be explained by the C02 production calculated to be associated with the enhanced conversion of these substrates to other products during acidosis. 'CO2 production from 1,2-"C-acetate was found to be significantly increased in alkalosis rather than acidosis. These studies suggest that in the rat, the rate at which glutamine is completely oxidized in the Krebs cycle is not a factor regulating renal ammonia production. A comparison of the effects of acidbase status on glutamine and glutamate metabolism suggests that either glutamine transport or glutamine transaminase activity are significantly increased by acidosis. Mr. Strope is a second year student at

show abstract

Renal protein and ammonia biochemistry in NH4Cl acidosis and after uninephrectomy

Cited by 24 publications

References 0 publications

Renal metabolic response to acid-base changes

Renal metabolic response to acid-base changes

Compensatory Growth and Other Growth Responses of the Kidney

The effects of acidosis and alkalosis on the metabolism of glutamine and glutamate in renal cortex slices

Contact Info

Product

Resources

About