Renal kallikrein mRNA localization by in situ hybridization

Xiong, William; Chao, Lee; Chao, Julie

doi:10.1038/ki.1989.130

Cited by 41 publications

(11 citation statements)

References 31 publications

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“…Kallikrein that produces bradykinin is biosynthesized in kidney cortex (Xiong et al, 1989). Kallikrein 7 (Klk7) is found in Table 5 showing 15-fold and 285-fold expression in medulla and cortex, respectively, compared with papilla.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Gene Expression Profiles Among Papilla, Medulla and Cortex in Rat Kidney

et al. 2006

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-The aim of this study was to compare gene expression profiles in the different kidney regions as the basis for toxicogenomics. Rat kidney was separated into papilla, medulla and cortex, and total RNA was isolated from these and from the whole slice. Gene expression profiling was performed using Affymetrix Rat Genome 230 2.0 Array. When global normalization was applied, the expression of β-actin or GAPDH varied among the regions. It was considered that such a comparison could not be made, especially between papilla and other portions, since the production of total mRNA in the former was relatively low. In fact, ANOVA was performed on the gene expression values with global normalization in papilla, medulla, cortex, and whole slice, and the numbers of genes appeared to be the highest in papilla. It was also observed that many genes showed their maximum or minimum in the whole slice, which was theoretically impossible. To overcome the problems associated with global normalization, the "percelome" normalization (a way to obtain the values directly related to the copies of mRNA per cell) was employed to compare the regions. In applying this procedure, probe sets with regional difference in expression were efficiently extracted by ANOVA. When they were sorted by the fold difference to other regions, the higher rank was occupied by genes characteristic of the functions of kidney, i.e., channels, transporters and metabolic enzymes. Some of them were consistent with the literature and were related to pathophysiological phenomena. Comprehensive comparison of data of gene expression in the renal anatomical area will greatly enhance studies of the physiological function and mechanism of toxicity in kidney.

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Section: Discussionmentioning

confidence: 99%

Comparison of Gene Expression Profiles Among Papilla, Medulla and Cortex in Rat Kidney

et al. 2006

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“…The recent finding of kallikrein mRNA in the distal renal tubule and at the vascular pole of the glomerulus suggests that an intrarenal kallikrein-kinin system may be involved in the regulation of blood pressure. 27 Decreased intrarenal activity of the kallikrein-kinin system and attendant decreases in prostaglandin synthesis might give rise to an increase in blood pressure by inducing a shift in the pressure-natriuresis curve.…”

Section: Resultsmentioning

confidence: 99%

Cosegregation of blood pressure with a kallikrein gene family polymorphism.

et al. 1991

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It has recently been proposed that sequence variation in the gene coding for tissue kallikrein might be involved in the pathogenesis of hypertension. However, molecular evidence of an association between a sequence alteration in the kallikrein gene family and the transmission of increased blood pressure has never been reported. In 32 recombinant inbred (RI) strains derived from the spontaneously hypertensive rat (SHR) and the normotensive Brown Norway rat (BN), we investigated whether a restriction fragment length polymorphism (RFLP) marking the kallikrein gene family cosegregated with blood pressure. In the RI strains that inherited the kallikrein RFLP from the SHR progenitor strain, the median systolic, diastolic, and mean arterial pressures were significantly greater than in the RI strains that inherited the kallikrein RFLP from the BN progenitor strain. These findings suggest that in the rat, sequence variation in the kallikrein gene family, or in closely linked genes, may have the capacity to affect blood pressure. (Hypertension 1991;17:242-246)

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“…Tissue kallikrein mRNA is expressed predominantly in the cells of the distal tubules and also in the vascular pole of the glomeruli (89) and in the CNT of the outer cortex (90). Another report (91) states that kallikrein is present in the granular peripolar cells of the glomeruli of the human kidney, but that its mRNA is not located at this site.…”

Section: Renal Kallikreinmentioning

confidence: 99%

A Missing Link Between a High Salt Intake and Blood Pressure Increase

Katori

Murakami

2006

J Pharmacol Sci

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Abstract. It is widely accepted that a high sodium intake triggers blood pressure rise. However, only one-third of the normotensive subjects were reported to show salt-sensitivity in their blood pressure. Many factors have been proposed as causes of salt-sensitive hypertension, but none of them provides a satisfactory explanation. We propose, on the basis of accumulated data, that the reduced activity of the kallikrein-kinin system in the kidney may provide this link. Renal kallikrein is secreted by the distal connecting tubular cells and all kallikrein-kinin system components are distributed along the collecting ducts in the distal nephron. Bradykinin generated is immediately destroyed by carboxypeptidase Y-like exopeptidase and neutral endopeptidase, both quite independent from the kininases in plasma, such as angiotensin converting enzyme. The salt-sensitivity of the blood pressure depends largely upon ethnicity and potassium intake. Interestingly, potassium and ATP-sensitive potassium (K ATP ) channel blockers accelerate renal kallikrein secretion and suppress blood pressure rises in animal hypertension models. Measurement of urinary kallikrein may become necessary in salt-sensitive normotensive and hypertensive subjects. Furthermore, pharmaceutical development of renal kallikrein releasers, such as K ATP channel blockers, and renal kininase inhibitors, such as ebelactone B, may lead to the development of novel antihypertensive drugs.

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Renal kallikrein mRNA localization by in situ hybridization

Cited by 41 publications

References 31 publications

Comparison of Gene Expression Profiles Among Papilla, Medulla and Cortex in Rat Kidney

Comparison of Gene Expression Profiles Among Papilla, Medulla and Cortex in Rat Kidney

Cosegregation of blood pressure with a kallikrein gene family polymorphism.

A Missing Link Between a High Salt Intake and Blood Pressure Increase

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