Renal Injury Is a Consistent Finding in Dutch Belted Rabbits Experimentally Infected with Enterohemorrhagic<i>Escherichia coli</i>

García, Alexis; Bosques, Carlos J.; Wishnok, John S.; Feng, Yan; Karalius, Brad J.; Butterton, Joan R.; Schauer, David B.; Rogers, Arlin B.; Fox, James G.

doi:10.1086/501364

Cited by 46 publications

(41 citation statements)

References 38 publications

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“…An important subclass of STEC is EHEC, which triggers the formation of AE lesions on the intestinal mucosa. Some rabbit and piglet models manifest both AE lesion formation and Stx-mediated disease (21,23,52,53,85), but no such model has yet been developed in conventional mice. We show here that C. rodentium lysogenized with λStx2dact, which produces a mucus-activatable Stx previously associated with severe disease in mice (59)(60)(61), provides a model that features prototypic AE lesions during intestinal colonization and Stx-mediated weight loss and death, recapitulating renal dysfunction observed in human HUS and murine toxin injection models (4,54).…”

Section: Discussionmentioning

confidence: 99%

A novel murine infection model for Shiga toxin–producing Escherichia coli

Mallick

McBee²,

Vanguri³

et al. 2012

J. Clin. Invest.

118

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Enterohemorrhagic E. coli (EHEC) is an important subset of Shiga toxin-producing (Stx-producing) E. coli (STEC), pathogens that have been implicated in outbreaks of food-borne illness and can cause intestinal and systemic disease, including severe renal damage. Upon attachment to intestinal epithelium, EHEC generates "attaching and effacing" (AE) lesions characterized by intimate attachment and actin rearrangement upon host cell binding. Stx produced in the gut transverses the intestinal epithelium, causing vascular damage that leads to systemic disease. Models of EHEC infection in conventional mice do not manifest key features of disease, such as AE lesions, intestinal damage, and systemic illness. In order to develop an infection model that better reflects the pathogenesis of this subset of STEC, we constructed an Stx-producing strain of Citrobacter rodentium, a murine AE pathogen that otherwise lacks Stx. Mice infected with Stx-producing C. rodentium developed AE lesions on the intestinal epithelium and Stx-dependent intestinal inflammatory damage. Further, the mice experienced lethal infection characterized by histopathological and functional kidney damage. The development of a murine model that encompasses AE lesion formation and Stx-mediated tissue damage will provide a new platform upon which to identify EHEC alterations of host epithelium that contribute to systemic disease.

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Section: Discussionmentioning

confidence: 99%

A novel murine infection model for Shiga toxin–producing Escherichia coli

Mallick

McBee²,

Vanguri³

et al. 2012

J. Clin. Invest.

118

View full text Add to dashboard Cite

show abstract

“…Numerous animal models have been developed, including mice, rats, chickens, rabbits, cows, greyhounds, baboons, and macaques (141,(173)(174)(175)(176)(177)(178). Although these models do not fully replicate all aspects of the STEC infection in humans, they provide valuable insight into intestinal colonization, STEC pathogenesis, immune response, and efficacy of possible treatment regimens (179,180).…”

Section: Animal Modelsmentioning

confidence: 99%

Animal Reservoirs of Shiga Toxin-ProducingEscherichia coli

2014

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Shiga toxin-producing Escherichia coli (STEC) strains have been detected in a wide diversity of mammals, birds, fish, and several insects. Carriage by most animals is asymptomatic, thus allowing for dissemination of the bacterium in the environment without detection. Replication of the organism may occur in the gastrointestinal tract of some animals, notably ruminants. Carriage may also be passive or transient, without significant amplification of bacterial numbers while in the animal host. Animals may be classified as reservoir species, spillover hosts, or dead-end hosts. This classification is based on the animal's ability to (i) transmit STEC to other animal species and (ii) maintain STEC infection in the absence of continuous exposure. Animal reservoirs are able to maintain STEC infections in the absence of continuous STEC exposure and transmit infection to other species. Spillover hosts, although capable of transmitting STEC to other animals, are unable to maintain infection in the absence of repeated exposure. The large diversity of reservoir and spillover host species and the survival of the organism in environmental niches result in complex pathways of transmission that are difficult to interrupt.

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“…For example, infection with strain RDEC-1, a well-described model of human EPEC infection (6), mimics a disease process seen in children with infectious gastroenteritis (45). Other recent studies have shown that Dutch Belted rabbits naturally or experimentally infected with EHEC strains exhibit bloody diarrhea and typhlocolitis, as well as nephropathy and renal thrombosis characteristic of the hemolytic-uremic syndrome (11,12,14).…”

mentioning

confidence: 99%

Enzootic Enteropathogenic Escherichia coli Infection in Laboratory Rabbits

et al. 2012

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bEnteropathogenic Escherichia coli (EPEC) is the most important cause of persistent diarrhea in children, particularly in developing countries. Animals serve as pathogenic E. coli reservoirs, and compelling evidence for cross-species EPEC transmission exists. In this report, enzootic EPEC infection associated with up to 10.5% diarrhea-associated morbidity in a large laboratory Dutch Belted rabbit colony was investigated. These rabbits were obtained from a commercial vendor and had acute diarrhea following shipment. Fecal culture of 20 rabbits yielded 48 E. coli isolates, 83% of which were eae positive. Repetitive sequencebased PCR (REP-PCR) and serologic analysis identified a single disease-associated EPEC O145:H2 strain. In sampled rabbits, EPEC-positive culture and the presence of diarrhea were significantly associated. This strain displayed a localized adherence-like HEp-2 cell adherence pattern, as seen in diarrheic human infant EPEC isolates. Treatment was instituted with the fluoroquinolone antibiotic enrofloxacin, to which all isolates were susceptible. Preshipment parenteral enrofloxacin administration reduced diarrhea-associated morbidity 22-fold and mortality 12-fold in subsequent deliveries. This report emphasizes the zoonotic potential of animal EPEC strains and the need for virulence determinant-based screening of E. coli isolates from diarrheic animals.

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Renal Injury Is a Consistent Finding in Dutch Belted Rabbits Experimentally Infected with EnterohemorrhagicEscherichia coli

Cited by 46 publications

References 38 publications

A novel murine infection model for Shiga toxin–producing Escherichia coli

A novel murine infection model for Shiga toxin–producing Escherichia coli

Animal Reservoirs of Shiga Toxin-ProducingEscherichia coli

Enzootic Enteropathogenic Escherichia coli Infection in Laboratory Rabbits

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