Renal Clearable Gold Nanoparticle-Functionalized Silk Film for in vivo Fluorescent Temperature Mapping

Abstract

Implantable optical sensing devices that can continuously monitor physiological temperature changes hold great potential toward applications in healthcare and medical field. Here, we present a conceptual foundation for the design of biocompatible temperature sensing device by integrating renal clearable luminescent gold nanoparticles (AuNPs) with silk film (AuNPs-SF). We found that the AuNPs display strong temperature dependence in both near-IR fluorescence intensity and lifetime over a large temperature range (10-60 • C), with a fluorescence intensity sensitivity of 1.72%/ • C and lifetime sensitivity of 0.09 µs/ • C. When integrated, the AuNPs with biocompatible silk film are implanted in the dorsal region of mice. The fluorescence imaging of the AuNPs-SF in the body shows a linear relationship between the average fluorescence intensity and temperature. More importantly, <3.68% ID gold are left in the body, and no adverse effect is observed for 8 weeks. This AuNPs-SF can be potentially used as a flexible, biocompatible, and implantable sensing device for in vivo temperature mapping.