Renal and Myocardial Histopathology and Morphometry in Rats with Adenine - Induced Chronic Renal Failure: Influence of Gum Acacia

Ali, Badreldin H.; Inuwa, Ibrahim Mohammed; Za’abi, Mohammed Al; Bahlani, Shadia Al; Issaei, Halima Al; Ramkumar, Aishwarya; Madanagopal, T.T.; Nemmar, Abedrrahim; Malheiros, Denise; Zatz, Roberto

doi:10.1159/000363045

Cited by 29 publications

(23 citation statements)

References 26 publications

(38 reference statements)

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“…The kidneys from the control and chrysintreated rats appeared normal. However, the kidneys of adenine-treated rats were pale and with white crystals, similar to that described before [14][15][16]. The appearance of the kidneys of rats treated with adenine plus the three doses of chrysin were improved compared with the kidneys of rats treated with adenine alone.…”

Section: Physiological Datasupporting

confidence: 80%

Therapeutic Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats

Ali

Za’abi

Adham

et al. 2016

Cell Physiol Biochem

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Background/Aims: To study the therapeutic effect of chrysin, a flavonoid with strong antioxidant and anti-inflammatory activities, on adenine-induced chronic kidney diseases (CKD) in rats. Methods: Chrysin, in three graded oral doses (10, 50 and 250 mg/kg), was given for 10 consecutive days to rats after the induction of CKD by feeding them adenine (0.25%^w/w for 35 days). Several plasma and urine biomarkers and tissues morphology were used the investigate chrysin effect on kidney structure and function. Results: Adenine lowered creatinine clearance and elevated the concentrations of urea, creatinine, plasma neutrophil gelatinase-associated lipocalin and urinary N-Acetyl-beta-D-glucosaminidase activity, and increased the concentrations of the uremic toxin indoxyl sulfate, in addition to some inflammatory cytokines. Renal histopathological markers of inflammation and fibrosis were significantly increased. Renal catalase and superoxide dismutase activities, total antioxidant capacity and reduced glutathione were all adversely affected. Most of these adenine - induced actions were moderately mitigated by chrysin, especially at the highest dose. Compared to control, chrysin did not cause any overt adverse effects on the treated rats. Conclusion: Different doses of chrysin produce variable therapeutic salutary effects in rats with CKD, and that, pending further studies, its usability as a possible therapeutic agent in human CKD should be considered.

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Section: Physiological Datasupporting

confidence: 80%

Therapeutic Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats

Ali

Za’abi

Adham

et al. 2016

Cell Physiol Biochem

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“…We have previously shown that induction of CKD with adenine in rats results in a significant increase in blood pressure and in histological and morphometric cardiac damage . In the present work, we have shown that curcumin was effective in dose‐dependently ameliorating the rise in blood pressure in rats with adenine‐induced CKD.…”

Section: Discussionsupporting

confidence: 65%

“…We have previously shown that induction of CKD with adenine in rats results in a significant increase in blood pressure [18] and in histological and morphometric cardiac damage [8].…”

Section: Discussionmentioning

confidence: 99%

Curcumin Ameliorates Kidney Function and Oxidative Stress in Experimental Chronic Kidney Disease

Ali

Al-Salam

Suleimani

et al. 2017

Basic Clin Pharma Tox

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134

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Chronic kidney disease (CKD) is known to involve inflammation, oxidative stress and apoptosis. Here, we investigated the impact of curcumin (diferuloyl methane, a phenolic turmeric pigment), which has strong antioxidant, anti-inflammatory and anti-apoptotic activities on kidney structure and function in rats with adenine-induced CKD. Rats were treated for 5 weeks with adenine to induce CKD-like renal damage and combined with three doses of curcumin. Markers of kidney function and oxidative stress were quantified in plasma, urine, renal homogenates and on kidney tissue. Curcumin was found to significantly abate adenine-induced toxic effects such as reduced creatinine clearance, elevated neutrophil gelatinase-associated lipocalin levels and raised urinary N-acetyl-b-D-glucosaminidase activities. Curcumin markedly reduced renal morphological damage and histopathological markers of inflammation, fibrosis and apoptosis. Curcumin further reduced adenine-induced hypertension, urinary albumin, the inflammatory cytokines IL-1b, IL-6 and TNF-a, cystatin C and adiponectin. It restored plasma sclerostin concentrations and lowered oxidative stress in renal homogenates. In animals treated with the two higher curcumin concentrations, alone or in combination with adenine, an increased expression of the antioxidative transcription factor Nrf2 was found as well as up-regulation of the activity of its direct target glutathione reductase, and of an indirect target, the glutathione level. In conclusion, curcumin exhibits salutary effects against adenine-induced CKD in rats by reducing inflammation and oxidative stress via up-regulation of the transcription factor Nrf2.Chronic kidney disease (CKD) is a major and growing public health problem in both developed [1] and developing countries [2]. CKD is considered a key determinant of the poor health outcome of major non-communicable diseases [3] because of the high prevalence of morbidity and mortality associated with it, mainly due to cardiovascular dysfunction. Till now, there is no drug to improve kidney function in patients with CKD. The current therapeutic approaches to slow down its progression are limited to normalization of insulin, glucose and blood pressure [4]. Therefore, the development of novel therapies to either slow or reverse the deterioration in kidney function is highly needed. In particular, interesting for this are natural products with proven safety profiles.The pathophysiological basis of CKD and its complications include inflammation, oxidative stress and apoptosis [5]. These features consistently occur in human beings and animals. They are also major mediators of the disease, exerting similar effects in chronic renal failure models in rats [6,7]. Patients and laboratory animals with CKD have high plasma concentrations of inflammatory mediators (such as C-reactive protein, tumour necrosis factor and other cytokines) and several markers of nitrosative and oxidative stress [8,9]. Turmeric (Curcuma longa) is a popular Asian spice that has been utilized for...

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“…Thus, adenine-induced CRF in rats was adopted as a disease model for the evaluation of the effect of H 2 S on CRF. The results showed that the rats fed on the adenine diet for 4 weeks showed increased water intake, urine production, urinary protein, and relative kidney weight, which were similar to the signs and symptoms in rats with adenine-induced CRF 41, 42 . In addition, a previous study has reported a reduction in body weight in adenine-fed rats which could be attributed to the reduced food consumption 43 .…”

Section: Discussionsupporting

confidence: 52%

Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways

Luo

Wang

et al. 2017

Sci Rep

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Chronic renal failure (CRF) is a major public health problem worldwide. Hydrogen sulfide (H2S) plays important roles in renal physiological and pathophysiological processes. However, whether H2S could protect against CRF in rats remains unclear. In this study, we found that H2S alleviated gentamicin-induced nephrotoxicity by reducing reactive oxygen species (ROS)-mediated apoptosis in normal rat kidney-52E cells. We demonstrated that H2S significantly improved the kidney structure and function of CRF rats. We found that H2S decreased the protein levels of Bax, Caspase-3, and Cleaved-caspase-3, but increased the expression of Bcl-2. Treatment with H2S reduced the levels of malondialdehyde and ROS and increased the activities of superoxide dismutase and glutathione peroxidase. H2S significantly abolished the phosphorylation of extracellular signal-regulated protein kinase 1/2, c-Jun N-terminal kinase, and p38 in the kidney of CRF rats. Furthermore, H2S decreased the expression levels of tumor necrosis factor-α, interleukin (IL)-6, IL-10, and monocyte chemoattractant protein-1, as well as the protein levels of p50, p65, and p-p65 in the kidney of CRF rats. In conclusion, H2S could ameliorate adenine-induced CRF in rats by inhibiting apoptosis and inflammation through ROS/mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways.

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Renal and Myocardial Histopathology and Morphometry in Rats with Adenine - Induced Chronic Renal Failure: Influence of Gum Acacia

Cited by 29 publications

References 26 publications

Therapeutic Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats

Therapeutic Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats

Curcumin Ameliorates Kidney Function and Oxidative Stress in Experimental Chronic Kidney Disease

Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways

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