Renal Alterations Promoted By The Lectins From Canavalia Ensiformis (Cona) And Dioclea Guianensis (Dguil) Seeds

Havt, Alexandre; Barbosa, Paula; Sousa, Ticiana Meireles; Martins, Alice Maria Costa; Nobre, Arlandia Cristina Lima; Nascimento, Kyria S.; Teixeira, Edson Holanda; Pinto, Vicente de Paulo Teixeira; Sampaio, Alexandre Holanda; Fonteles, M. C.

doi:10.2174/0929866033479130

Cited by 11 publications

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“…It has been shown that plant and animal lectins may promote inflammatory mediator release and that prostaglandin might interfere in sodium, potassium and chloride tubular transportation (27,28). In our experiments, indomethacin reversed the changes in the tubular transport of electrolytes induced by BmLec, suggesting the participation of eicosanoids in the process.…”

Section: Discussionsupporting

confidence: 61%

“…In our experiments, indomethacin reversed the changes in the tubular transport of electrolytes induced by BmLec, suggesting the participation of eicosanoids in the process. In addition, the renal cortex produces prostaglandins that may inhibit tubular sodium reabsorption in the thick ascending limb of the loop of Henle and reduce chloride transport, thus exerting an influence on renal blood flow and glomerular filtration rate (28,29).…”

Section: Discussionmentioning

confidence: 99%

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Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Barbosa¹,

Martins²,

Toyama³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis pv. passiflorae treated with lectin disclosed a high vesiculation and membrane rupture. BmLec induced a strong and significant increase in insulin secretion at 2.8 and 16.7 mM glucose concentrations, and this effect was seen in the presence of EGTA in both experiments. BmLec (10 µg/mL) increased the perfusion pressure, renal vascular resistance and urinary flow. The glomerular filtration rate and percentages of sodium, potassium and chloride tubular transport were reduced at 60 minutes of perfusion. Renal alterations caused by BmLec were completely inhibited by indomethacin in all evaluated parameters. In conclusion, the C-type lectin isolated from Bothrops moojeni affected platelet aggregation, insulin secretion, antibacterial activity and isolated kidney function

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Barbosa¹,

Martins²,

Toyama³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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“…In respect to renal effects of Diocleinae lectins, our group demonstrated that the lectin of Canavalia brasiliensis (ConBr) (Teixeira et al, 2001), DguiL and ConA (Havt et al, 2003) distinctly alter physiological parameters in ex vivo rat kidneys. Considering that kidneys are responsible for the maintenance and excretion of electrolytes in the human body and the structure-activity relationship among Diocleinae lectins, the direct renal effects promoted by the seed lectin of Cratylia floribunda in isolated perfused kidneys and mesenteric bed of rats were investigated.…”

Section: Introductionmentioning

confidence: 99%

The effect of Cratylia floribunda lectin on renal hemodynamics and ion transport

Havt

Assreuy

Nascimento

et al. 2015

Braz. J. Pharm. Sci.

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Lectins have been described as glycoproteins that reversibly and specifically bind to carbohydrates. Legume lectins isolated from the subtribe Diocleinae (Canavalia, Dioclea and Cratylia) are structurally homologous with respect to their primary structures. The Diocleinae lectins of Canavalia brasiliensis, Dioclea guianensis and Canavalia ensiformis have been shown to distinctly alter physiological parameters in isolated rat kidneys. Thus, the aim of this study was to investigate the effect of Cratylia floribunda lectin (CFL) on renal hemodynamics and ion transport in rats. In isolated perfused kidneys, CFL (10 mg/mL, n=5) increased RPP, RVR and decreased %TK+, but did not change urinary flow, glomerular filtration rate, sodium or chloride tubular transport. In isolated perfused mesenteric bed, CFL (3 and 10 mg/mL/min; n=4) did not alter tissue basal tonus or tissue contraction by phenylephrine (1 mM/mL/min).In conclusion, the seed lectin of Cratylia floribunda increased renal hemodynamic parameters showing a kaliuretic effect. This effect could be of tubular origin, rather than a result from haemodynamic alterations.Uniterms: Plant lectin. Amiloride. Cratylia floribunda/lectina/renal hemodynamics. Renal hemodynamics. Ion-transport.As lectinas são descritas como (glico)proteínas que se ligam, especificamente e reversivelmente, a carboidratos. Lectinas de leguminosas isoladas da subtribo Diocleinae (Canavalia, Dioclea e Cratylia) são estruturalmente homólogas em relação às suas estruturas primárias. Demonstrou-se que as lectinas de Diocleinae Canavalia brasiliensis, Dioclea guianensis e Canavalia ensiformis alteram diferentemente parâmetros fisiológicos em rins isolados de ratos. Dessa maneira, o objetivo deste estudo foi investigar o papel da lectina de Cratylia floribunda (CFL) na hemodinâmica renal e no transporte de íons em ratos. Em rins isolados perfundidos, CFL (10 mg/mL, n=5) aumentou a pressão de perfusão renal, a resistência vascular renal e reduziu o percentual do transporte tubular de K +

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“…Despite the high degree of identity observed between the lectins isolated from seeds of the Diocleinae subtribe, they exhibit different biological activities (Alencar et al, 1999;Andrade et al, 1999;Assreuy et al, 1999;Cavada et al, 2001;Havt et al, 2003;Lopes et al, 2005). For instance, although concanavalin A (ConA) and Canavalia maritima lectin (ConM) possess 98% identity in primary sequence, they present different histamine-releasing activities, which is related to their affinity for biantennary complex oligosaccharides (Dam et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Crystallization and preliminary X-ray diffraction analysis of the lectin fromCanavalia bolivianaPiper seeds

Moura

Bezerra

et al. 2009

Acta Cryst Sect F

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Plant lectins are the most studied group of carbohydrate-binding proteins. Despite the high similarity between the members of the Diocleinae subtribe (Leguminosae) group, they present differing biological activities. Canavalia boliviana lectin (Cbol) was purified using a Sephadex G-50 column and crystallized in the presence of X-Man by hanging-drop vapour diffusion at 293 K. After optimization, crystals suitable for diffraction were obtained under the condition 0.1 M HEPES pH 7.5 and 3.0 M sodium formate. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 126.70, b = 66.64, c = 64.99 Å , = 90.0, = 120.8, = 90.0 . Assuming the presence of a dimer in the asymmetric unit, the solvent content was estimated to be about 46%. A complete data set was collected at 1.5 Å resolution.

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Renal Alterations Promoted By The Lectins From Canavalia Ensiformis (Cona) And Dioclea Guianensis (Dguil) Seeds

Cited by 11 publications

References 0 publications

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

The effect of Cratylia floribunda lectin on renal hemodynamics and ion transport

Crystallization and preliminary X-ray diffraction analysis of the lectin fromCanavalia bolivianaPiper seeds

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