Removing endosymbiotic Wolbachia specifically decreases lifespan of females and competitiveness in a laboratory strain of Drosophila melanogaster

Alexandrov, I. D.; Alexandrova, Margarita V.; Goryacheva, I. I.; Rochina, N. V.; Шайкевич, Е. В.; Захаров, И. К.

doi:10.1134/s1022795407100080

Cited by 43 publications

(36 citation statements)

References 24 publications

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“…We find that germ-free flies are long-lived, consistent with studies reporting that axenic flies show improved markers of intestinal homeostasis during aging (Broderick et al, 2014; Buchon et al, 2009; Guo et al, 2014) and the growing body of evidence indicating that intestinal homeostasis is a critical determinant of fly lifespan (Rera et al, 2013; Wang et al, 2014). Inconsistent reports of the impact of bacterial exposure on fly lifespan extend to the impact of single bacterial species, with the Drosophila endosymbiont Wolbachia having been reported to have a positive (Aleksandrov et al, 2007; Ikeya et al, 2009), negative (Min and Benzer, 1997), or neutral impact on lifespan (Ikeya et al, 2009), dependent on the strain of Wolbachia and the genotype of the host. While the w 111 8 female flies used in this study contain Wolbachia , given the low representation of Wolbachia sequences in our metagenomic data and the absence of an increase in Wolbachia abundance following intestinal barrier dysfunction, it is unlikely that the presence of Wolbachia alone can explain our results.…”

Section: Discussionmentioning

confidence: 99%

Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality

et al. 2015

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Summary Alterations in the composition of the intestinal microbiota have been correlated with aging and measures of frailty in the elderly. However, the relationships between microbial dynamics, age-related changes in intestinal physiology and organismal health remain poorly understood. Here, we show that dysbiosis of the intestinal microbiota, characterized by an expansion of the Gammaproteobacteria, is tightly linked to age-onset intestinal barrier dysfunction in Drosophila. Indeed, alterations in the microbiota precede and predict the onset of intestinal barrier dysfunction in aged flies. Changes in microbial composition occurring prior to intestinal barrier dysfunction contribute to changes in excretory function and immune gene activation in the aging intestine. In addition, we show that a distinct shift in microbiota composition follows intestinal barrier dysfunction leading to systemic immune activation and organismal death. Our results indicate that alterations in microbiota dynamics could contribute to and also predict varying rates of health decline during aging in mammals.

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Section: Discussionmentioning

confidence: 99%

Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality

et al. 2015

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“…Furthermore, a variety of environmental factors have to be considered when comparing lifespan between populations. These include diet[41], [68], larval densities, Wolbachia infection status [39], [69], mating status and gender cohabitation [70], [71], temperature, humidity and circadian rhythm. We discuss the practical implications of these genetic and environmental considerations below.…”

Section: Practical Aspects For Studying Aging In Fliesmentioning

confidence: 99%

“…Infection with the parasitic intracellular bacterium Wolbachia can have significant (positive or negative, depending on genetic backgrounds) effects on lifespan [39], [69], [75]. It is therefore critical to establish the Wolbachia infection status of tested fly lines (which can be achieved through simple PCR assays or by DAPI staining of selected tissues [76]).…”

Section: Practical Aspects For Studying Aging In Fliesmentioning

confidence: 99%

Studying aging in Drosophila

Jasper

2014

Methods

120

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Drosophila melanogaster represents one of the most important genetically accessible model organisms for aging research. Studies in flies have identified single gene mutations that influence lifespan and have characterized endocrine signaling interactions that control homeostasis systemically. Recent studies have focused on the effects of aging on specific tissues and physiological processes, providing a comprehensive picture of age-related tissue dysfunction and the loss of systemic homeostasis. Here we review methodological aspects of this work and highlight technical considerations when using Drosophila to study aging and age-related diseases.

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“…E stimated to infect more than 65% of all insect species, Wolbachia pipientis are maternally inherited, Gram-negative endosymbiotic bacteria (1, 2). They manipulate host reproductive systems through a variety of strategies, including cytoplasmic incompatibility, male killing, feminization, and parthenogenesis (3), and also provide direct mutualistic benefits to hosts in certain contexts (4)(5)(6). Recently, a life-shortening W. pipientis strain (wMelPop-CLA) was successfully introduced into Aedes aegypti (7), the main mosquito vector of dengue viruses.…”

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confidence: 99%

Wolbachiauses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vectorAedes aegypti

Hussain

Frentiu

Moreira

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

218

245

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The obligate endosymbiont Wolbachia pipientis is found in a wide range of invertebrates where they are best known for manipulating host reproduction. Recent studies have shown that Wolbachia also can modulate the lifespan of host insects and interfere with the development of human pathogens in mosquito vectors. Despite considerable study, very little is known about the molecular interactions between Wolbachia and its hosts that might mediate these effects. Using microarrays, we show that the microRNA (miRNA) profile of the mosquito, Aedes aegypti, is significantly altered by the wMelPop-CLA strain of W. pipientis. We found that a host miRNA (aae-miR-2940) is induced after Wolbachia infection in both mosquitoes and cell lines. One target of aae-miR-2940 is the Ae. aegypti metalloprotease gene. Interestingly, expression of the target gene was induced after Wolbachia infection, ectopic expression of the miRNA independent of Wolbachia, or transfection of an artificial mimic of the miRNA into mosquito cells. We also confirmed the interaction of aae-miR-2940 with the target sequences using GFP as a reporter gene. Silencing of the metalloprotease gene in both Wolbachia-infected cells and adult mosquitoes led to a significant reduction in Wolbachia density, as did inhibition of the miRNA in cells. These results indicate that manipulation of the mosquito metalloprotease gene via aae-miR-2940 is crucial for efficient maintenance of the endosymbiont. This report shows how Wolbachia alters the host miRNA profile and provides insight into the mechanisms of host manipulation used by this widespread endosymbiont.

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Removing endosymbiotic Wolbachia specifically decreases lifespan of females and competitiveness in a laboratory strain of Drosophila melanogaster

Cited by 43 publications

References 24 publications

Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality

Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality

Studying aging in Drosophila

Wolbachiauses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vectorAedes aegypti

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