Removing Antinutrients from Rapeseed Press-Cake and Their Benevolent Role in Waste Cooking Oil-Derived Biodiesel: Conjoining the Valorization of Two Disparate Industrial Wastes

Abstract

Valorization of oilseed processing wastes is thwarted due to the presence of several antinutritional factors such as phenolics, tannins, glucosinolates, allyl isothiocyanates, and phytates; moreover, literature reporting on their simultaneous extraction and subsequent practical application is scanty. Different solvent mixtures containing acetone or methanol pure or combined with water or an acid (hydrochloric, acetic, perchloric, trichloroacetic, phosphoric) were tested for their efficiency for extraction of these antinutritive compounds from rapeseed press-cake. Acidified extraction mixtures (nonaqueous) were found to be superior to the nonacidified ones. The characteristic differences in the efficacy of these wide varieties of solvents were studied by principal component analysis, on the basis of which the mixture 0.2% perchloric acid in methanol/acetone (1:1 v/v) was deemed as "the best" for detoxification of rapeseed meal. Despite its high reductive potential, hemolytic activity of the extract from this solvent mixture clearly indicated the toxicity of the above-mentioned compounds on mammalian erythrocytes. Because of the presence of a high amount of antinutritive antioxidants, the study was further extended to examine the influence of this solvent extract on the stability of waste cooking oil-derived biodiesel. Treatment with the extract harbored significant improvement (p < 0.05) in the induction periods and pronounced reduction in microbial load of stored biodiesel investigated herein. Thus, a suitable solvent system was devised for removing the major antinutrients from rapeseed press-cake, and the solvent extract can, thereafter, be used as an effective exogenous antioxidant for biodiesel. In other words, integrated valorization of two different industrial wastes was successfully achieved.

Macroalgae as a sustainable aquafeed ingredient

“…Solvent extraction is a useful tool in removal of antinutrients such as tannins, phytic acid and isothiocyanate in plant meals (Das Purkayastha et al . ), or even to decrease the amount of other unwanted compounds in feed materials, for example carotenoids in maize meal (Park et al . ) and oil content in camelina meal (Ye et al .…”

Emerging technologies for the recovery of isothiocyanates, protein and phenolic compounds from rapeseed and rapeseed press-cake: Effect of high voltage electrical discharges

“…Although plant polyphenols are usually considered as valuable natural antioxidants, not all phenolics present in foods are beneficial, and they can even reduce the palatability and astringency of rapeseed products, and can act as antinutritional compounds for ruminants (Das Purkayastha et al, 2013).…”

“…However, most of them involve the use of different solvent mixtures containing acetone or methanol pure or combined with water or an acid (hydrochloric, acetic, perchloric, trichloroacetic, phosphoric) (Das Purkayastha et al, 2013) which are not in correspondence with the green extraction concept based on the use of renewable plant resources and alternative green solvents, reduction of energy consumption and unit operations, production of high quality and purity of extracts (non-denatured and biodegradable) and extracts co-products instead of wastes (Chemat, Vian, & Cravotto, 2012).…”

Development of a gelatin‐g‐poly(acrylic acid‐co‐acrylamide)–montmorillonite superabsorbent hydrogels for in vitro controlled release of vitamin B12

“…The hemolytic activity assay was performed with a protocol described by Das Purkayastha et al . with a slight modification.…”

“…The hemolytic activity assay was performed with a protocol described by Das Purkayastha et al 38 with a slight modification. In brief, in a centrifuge tube containing trisodium citrate (3.2%), fresh goat blood was collected.…”