Biosynthesis of nanomaterials has piqued the interest of current researchers due to its low toxicity, reproducibility, environmental friendliness, economic viability, and low energy consumption. In this direction, for the first time, the ability of biosynthesis titanium dioxide nanoparticle prepared from Zizyphus-Spina-Christi leaves (Z-TiO2) to degrade ciprofloxacin(CIP) residue from aqueous solution by changing different initial parameters pH (3,5,7,9 and 11), CIP initial conc. (15,25,35,45,and 55) mg/L, Z-TiO2 concentration (15,25,35,45,and 55) mg/L, H2O2 conc. (50,75,100,200,400 and 600) mg/L, and temperature of (25,40,and 60) °C, and the results confirm the effectiveness of Z- TiO2 as that of commercial TiO2 to remove CIP, with a maximum removal percentage at 120 min of 91 % at (5,15 mg/L, 25 mg/L, 100 mg/L, and 25°C), respectively. Furthermore, the investigations demonstrated that Z-TiO2 might be employed as a potential for up to six cycles of photocatalytic oxidation of organic pollutants before it loses efficiency. Its usage as a catalyst is viable because of its low cost and great output rate.