Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential

Arrighi, Corinne de Vantéry; Lucas, H. L.; Chardonnens, Didier; Agostini, Ariane de

doi:10.1186/1477-7827-7-1

Cited by 100 publications

(86 citation statements)

References 86 publications

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“…This finding contrasts with the results obtained for human semen, where a higher level of morphologically normal spermatozoa is observed in unbound fractions compared to control samples after MACS separation Said et al 2006;Aziz et al 2007). In other studies, however, human spermatozoa were not evaluated according to their morphology (De Vantery Arrighi et al 2009;Lee et al 2010) and no information on morphological changes is available for rabbit spermatozoa following MACS (Vasicek et al 2011). The majority of authors report an increase of human sperm motility in unbound fractions after MACS separation (De Vantery Arrigi et al 2009;Rawe et al 2010).…”

Section: Discussioncontrasting

confidence: 55%

Elimination of apoptotic boar spermatozoa using magnetic activated cell sorting

et al. 2014

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One of the features of apoptosis is the externalization of phosphatidylserine which could be used to remove apoptotic cells from semen preparations. Magnetic-activated cell sorting using annexin V-conjugated microbeads which bind to phosphatidylserine could be used to enhance semen quality. Twelve boar semen samples after 3 days of liquid storage at 16 -17 °C were subjected to magnetic-activated cell sorting. Bound and unbound fractions and control samples were subjected to flow cytometry following the staining of spermatozoa with Annexin V conjugated with Alexa Fluor 488 and propidium iodide. Four subpopulations were obtained: live, early apoptotic live, late apoptotic, early necrotic dead and late necrotic dead. The frequency of early apoptotic and late necrotic spermatozoa was significantly higher (P < 0.05) in bound (14.1 ± 10.6% and 24.1 ± 10.2%, respectively) than in unbound fractions (3.4 ± 2.1% and 12.7 ± 3.1%) and control (3.5 ± 1.6% and 12.0 ± 5.0%). The lowest concentration of live spermatozoa was found in the bound fraction (10.6 ± 8.0 %), which differed significantly (P < 0.05) from the control. In unbound fractions there was a significantly higher concentration (P < 0.05) of morphologically normal spermatozoa (31.8 ± 12.6%) compared to bound ones (5.9 ± 7.3%). A significantly (P < 0.05) lower proportion of morphologically normal spermatozoa was observed in both fractions compared to control (67.2 ± 17.0%). Boar spermatozoa were separated by the above method for the first time, however, the results showed this method to be inappropriate for boar semen separation under the tested conditions. Semen, short-term storage, MACS, apoptosis

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Section: Discussioncontrasting

confidence: 55%

Elimination of apoptotic boar spermatozoa using magnetic activated cell sorting

et al. 2014

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“…MACS efficiently reduces sperm DNA fragmentation levels [13,19,29,30,40,47,48] and effectively separates apoptotic from non-apoptotic spermatozoa [6, 8, 11, 14, 17-20, 28, 29, 34, 37-39, 44]. This selection leads to an improvement in sperm quality and functionality [14,19,23] because MACS positively affects sperm motility [5,8,29,34,38,41] and morphology as determined by the sperm deformity index [2-4, 38, 39, 41]. Several authors reported an improvement in fertilization rates [12,36] and embryo quality [1,9,40] because the best sperms were selected using MACS compared with standard selection methods.…”

Section: Discussionmentioning

confidence: 99%

Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis

Gil

Sar-Shalom

Sivira

et al. 2013

J Assist Reprod Genet

127

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Purpose To determine whether the use of Magnetic Activated Cell Sorting (MACS) as a sperm selection technique improves ART success rates in couples undergoing assisted reproduction treatment. Methods Systematic review and meta-analysis of prospective randomized trials. Two reviewers conducted study selection and data extraction independently. Results Five studies (prospective randomized trials) that comprised 499 patients were included. Sperm selection using MACS resulted in statistically significant differences in pregnancy rates when compared with density gradient centrifugation and swim-up techniques (RR= 1.50, 95 % CI 1.14-1.98). No differences were found between the groups according to the implantation (RR= 1.03, 95 % CI 0.80-1.31) and miscarriage (RR=2. 00, rates. Conclusions MACS appears to be a safe and efficient method to select functional sperm with consistently good results. This technique may improve pregnancy rates when used to complement standard sperm selection methods in ART.

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“…They suggested that not all species are suitable for MACS separation method. In our knowledge, most of the research for MACS separation or the combination of DGC and MACS used human samples (de Vantéry Arrighi, Lucas, Chardonnens, & De Agostini, 2009;Gil, Shalom, Sivira, Carreras, & Checa, 2013;Martin et al, 2017;Romany et al, 2017). DGC has been proven to effectively remove nonviable spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Density Gradient Centrifugation and Magnetic-Activated Cell Sorting with the Combination of Both on Bovine Spermatozoa Kinematics, Viability and Acrosome Status

Osman

Bakri

Jaffar

et al. 2017

JAS

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Conventional and novel spermatozoa separation techniques have their own strength and weaknesses. Both are widely used in assisted reproductive technologies (ARTs). In this study, combinations of two separation methods against single separation methods were evaluated. Freshly ejaculated semen was collected and divided into five groups; control, density gradient centrifugation (DGC), magnetic activated cell sorting (MACS), DGC-MACS and MACS-DGC; based on the sequence of separation. Evaluation of spermatozoa kinematics, viability and acrosome status for each group were done by computer assisted semen analysis (CASA), hypo-osmotic swelling test (HOST) and chlorotetracycline (CTC) staining respectively. Results show that DGC yield the fastest spermatozoa velocity and highest percentage of viable spermatozoa fraction compared to other groups. However, they also produced a high percentage of acrosome-reacted spermatozoa. Spermatozoa isolated through DGC-MACS shows the highest percentage of non-capacitated spermatozoa. However, spermatozoa isolated through DGC-MACS yields slower spermatozoa velocity population and lower percentage of viable sperm compared to spermatozoa isolated through DGC. Isolation of spermatozoa through MACS-DGC yields slower population of spermatozoa velocity with a lower percentage of non-capacitated spermatozoa compared to spermatozoa isolated through DGC-MACS. DGC separation and its combination with MACS had yield different spermatozoa quality. This distinct characteristic is likely suitable for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure respectively.

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Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential

Cited by 100 publications

References 86 publications

Elimination of apoptotic boar spermatozoa using magnetic activated cell sorting

Elimination of apoptotic boar spermatozoa using magnetic activated cell sorting

Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis

The Effect of Density Gradient Centrifugation and Magnetic-Activated Cell Sorting with the Combination of Both on Bovine Spermatozoa Kinematics, Viability and Acrosome Status

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